My colleague Micheline Fromont-Racine's manuscript about major redistribution of the interactions for the major RNA exonuclease Xrn1 when growth conditions change is online:

https://www.biorxiv.org/content/10.1101/2023.07.19.549689v1

#rna #xrn1 #yeast #preprint #biorxiv

@cian yes! my paper on the role of the "cytoplasmic" #RNA exonuclease #Xrn1 in #transcription came from the unexpected observation that steady-state #mRNA levels did NOT increase in the xrn1 deletion strain.
https://www.sciencedirect.com/science/article/pii/S0092867413005771

The mechanisms linking RNA degradation in the cytoplasm with DNA #transcription in the nucleus are unknown. An increase in RNA levels when degradation is impaired can affect the availability of RNA binding proteins for their function, including their ability to travel from the cytoplasm to the nucleus. A recent hypothesis is that the exonuclease #Xrn1 has nuclear localization signals that could be involved:

https://pubmed.ncbi.nlm.nih.gov/36418294/

@naturecomms During my PhD, I discovered that #Xrn1, the exonuclease that degrades mRNAs from start to end, is not solely cytoplasmic like everyone thought. In fact, I found it is shuttling between the nucleus and cytoplasm, together with other mRNA degradation enzymes. And these enzymes participate in #transcription (!) i.e. the enzymes that degrade mRNA also help create new mRNA.
This was coined as “mRNA buffering” by Cramer lab.
https://www.cell.com/fulltext/S0092-8674(13)00577-1
But how does Xrn1 get to the nucleus?