CellBioNews · @cellbionews
89 followers · 1693 posts · Server scientificnetwork.de

VertU: universal multilocus primer sets for eDNA metabarcoding of vertebrate diversity, evaluated by both artificial and natural cases

frontiersin.org/articles/10.33

#Metabarcoding #Vertebrate #primers #universal #16s #12s #coi

Last updated 1 year ago

Does phylogeny explain bias in quantitative DNA metabarcoding?
mbmg.pensoft.net/article/10126

#Metabarcoding #Phylogeny #quantification #16s #coi

Last updated 1 year ago

IMPACTT Microbiome · @IMPACTT
75 followers · 30 posts · Server mstdn.science
Jessica Rieder · @jess_rieder
111 followers · 179 posts · Server ecoevo.social

1) data is adequate for exploring the spatio-temporal dynamics and community composition at higher taxonomic levels (Fig 1), but not at lower levels as primer biases start to emerge (Fig 4)

Based off finding 1, it should be possible to combine rRNA data even when different 16S rRNA primers were used when exploring -temporal patterns.

#shortread #16s #spatio

Last updated 2 years ago

Jessica Rieder · @jess_rieder
111 followers · 177 posts · Server ecoevo.social

🚨 New Paper 🚨 and metabarcoding experimental choices and their impact on characterization in recirculating systems

Open access link to paper in Environmental Microbiomes:
rdcu.be/c5D08

health

See thread 🧵 for findings....

#Metagenomics #microbial #community #Freshwater #aquaculture #aquacultureeurope #16s #16smetabarcoding #fish #16srrnasequencing #RAS #microbiomes #bacteria

Last updated 2 years ago

Christian Diener · @thaasophobia
31 followers · 2 posts · Server mstdn.science

Pack your bags folks, it's decided. /s

#chatgpt3 #16s #Metagenomics

Last updated 2 years ago