Intragenomic diversity of the V9 hypervariable domain in eukaryotes has little effect on metabarcoding

https://www.cell.com/iscience/pdf/S2589-0042(23)01368-8.pdf

#18S #v9 #IntragenomicVariation #DADA2 #SWARM #metabarcoding

"Evaluating the diversity of the enigmatic fungal phylum Cryptomycota across habitats using 18S rRNA metabarcoding"

https://www.sciencedirect.com/science/article/abs/pii/S1754504823000259

#cryptomycota #18S #metabarcoding #diversity #fungi #mycology

Today is the LAST DAY to apply for our joint CBW-IMPACTT Microbiome Analysis Workshop at the early-bird rate! July 5-7, 2023 at the University of Calgary. Apply here: https://bioinformatics.ca/workshops-all/2023-cbw-impactt-microbiome-analysis/

#bioinformatics #workshop #16S #18S #sequencing #shotgunsequencing #metagenomics #genomics #transcriptomics #metatranscriptomics #longread #markergene #functionalannotation #tutorial #statistics #biostatistics #microbiome #microbiota #microbialecology #diversity #betadiversity #differentialabundance

Sunrise over Tropical Cyclone #18S near Western Australia. Winds reaching 95 km/h.

Tropical Cyclone #18S near Western Australia earlier today

Sunrise over Tropical Cyclone #18S near Western Australia. Winds close to 95 km/h.

RT @MikeAdcockWx
New 1500 UTC advisory released from JTWC on #18S // #23U. Still forecast to become a powerful cyclone prior to landfall in Western Australia.

Recent satellite picture of Tropical Cyclone #18S in the Timor Sea

Tropical Cyclone #18S in the Timor Sea last night

Tropical Cyclone #18S has been designated in the Timor Sea.

Follow updates in real-time here: https://zoom.earth/storms/18s-2023/

RT @PR2database Announcing the release of version 4.14.1 of the PR2 database of 18S eukaryotic reference sequences.

The database itself is unchanged but we now provide a web interface:
app.pr2-database.org
More information: https://pr2database.github.io/pr2database/articles/vignette-shiny-presentation.html. #protists #metabarcoding #database #18S.

RT @PR2database
Announcing the release of version 4.14.1 of the PR2 database of 18S eukaryotic reference sequences.

The database itself is unchanged but we now provide a web interface:

https://app.pr2-database.org

More information: https://pr2database.github.io/pr2database/articles/vignette-shiny-presentation.html

#protists #metabarcoding #database #18S

RT @PR2database@twitter.com

Announcing the release of version 4.14.1 of the PR2 database of 18S eukaryotic reference sequences.

The database itself is unchanged but we now provide a web interface:

https://app.pr2-database.org

More information: https://pr2database.github.io/pr2database/articles/vignette-shiny-presentation.html

#protists #metabarcoding #database #18S

🐦🔗: https://twitter.com/PR2database/status/1596067802216992768

Announcing the release of version 4.14.1 of the PR2 database of 18S eukaryotic reference sequences.

The database itself is unchanged, but we now provide a web interface:

https://app.pr2-database.org

More information: https://pr2database.github.io/pr2database/articles/vignette-shiny-presentation.html

#protists #metabarcoding #database #18S

RT @PR2database
Announcing the release of version 2.0 of metaPR2 database of 18S eukaryotic metabarcodes

https://shiny.metapr2.org/metapr2/

#protists #metabarcoding #biogeography #18S

Announcing the release of version 2.0 of metaPR2, database of 18S eukaryotic metabarcodes

https://shiny.metapr2.org/metapr2/

Major changes are listed here: https://pr2database.github.io/metapr2-shiny/news/index.html

* Addition of 18 new datasets
* Use of clustered metabarcodes (ASVs)
* New panel listing taxonomy of metabarcodes
* Barplots now include samples where taxa are absent

Please report any issue here: https://matrix.to/#/#pr2-database:matrix.org

#protists #metabarcoding #biogeography #18S

New paper on #foraminfera making use of the #pr2database.

#protists #metabarcoding #18S

https://www.nature.com/articles/s41467-022-34794-5

If you are interested in hearing the latest news and development of the PR2 database ecosystem for 18S rRNA metabarcoding and/or contribute to PR2, please join us at

https://matrix.to/#/#pr2-database:matrix.org

#18S #metabarcoding #protists

EukRibo a new database of 18S sequences developed from the PR2 database

https://www.biorxiv.org/content/10.1101/2022.11.03.515105v1

#18S #protists #metabarcoding

Usually working with sequencing 16S but I want to try out 18S with a student coming in. We would like to try to find primer/primer combinations which work best for different environments we are working in, e.g. sediment, biofilm, baltic sea water and deep biosphere (low yield of DNA). We have a bunch of primers and set ups at our department, which we will try out, but I thought I would give it a shot here as well. So if anyone has any recommendations:) Thanks #18S #sequencing #TipsAndTricks