AT1 alveolar cell-derived #SonicHedgeHog recruits secondary crest myofibroblasts, a transient (arising e15.5; diminished P60) Force-exerting mesenchyme lineage, to build a Mechanical niche for🐭Lung #Alveologenesis

Dr. Edward Morrisey Lab @sciencemagazine 2021
https://www.science.org/doi/10.1126/science.abc3172

SCAPE: a powerful bioinformatic framework integrating scRNAseq+scATACseq to explore cell communication

Sender cell Transcription factor-Ligand - Receiver Receptor-Transcription factor

#Angiocrine Lung #Alveologenesis

Cyp26b1, catabolizing #RetinoicAcid, is #EndothelialCell-restricted in 🐭 emb Lung+Kidney

Cyp26b1 KO->
⏬AT1 fate of Sox9+ distal airway epithelial progenitors
⏫septa thickness
⏬Airspace

@ondinecleaver2022 @Dev_journal 2020
https://journals.biologists.com/dev/article/147/4/dev181560/223218/Cyp26b1-is-an-essential-regulator-of-distal-airway

Fantastic story
AT1-#EndothelialCell crosstalk in Lung #Alveologenesis🤠

Hopx+ AT1 Vegfa KO->
Loss of Car4+ EC ramifying over Alveoli
w/o reduced PLVAP+ capillary EC->
Alveolar Smoothening (⏬Outpocketing?)🧐

Dr. Jichao Chen lab Dev Cell 2020
https://www.cell.com/developmental-cell/fulltext/S1534-5807(20)30010-1

AT1 cell Plasticity in #Alveologenesis & Lung Homestasis+Regeneration

AT1 contributes to 30% & 10% AT2 in neonatal & adult 🐭Hyperoxia Lung injury👹

TAZ (likely > #YAP) maintains adult AT1 lineage identity Fig 6E

Dr. David Frank & Edward Morrisey Labs @CellStemCell 2021
https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(21)00184-3