Epigenetics #PulmonaryArterialHypertension

JMJD1C a histone demethylase
⏫p-STAT3->
⏫#Glycolysis HK2/PGK1/LDHA->
⏫PA #SmoothMuscleCell Proliferation

Serum Lactate⏬by AAV2-shJMJD1C in🐭#Hypoxia-PAH
Local effect on #EndMT?

Dr H Zhao lab Cell Death Discovery 2023
https://www.nature.com/articles/s41420-023-01390-5

#ArterioVenousFistula #EndMT

Low #OscillatoryShearStress->
⏫Osteopontin-CD44 in #EndothelialCell->
⏫EndMT->
⏫24% of neointimal cells at rat aortocaval junction

A cascade independent of #HyaluronicAcid🤓

Dr. Yung-Hsin Yeh lab Kidney Int 2023
https://www.kidney-international.org/article/S0085-2538(23)00011-X/fulltext

Partial #EndMT,⏫by #Hypoxia-induced CD45, as a major cellular source for neointima formation

50% ECs in ligated artery are CD45+

CD45 is required to maintain this Partial status
Amazing!🤩

Dr. Yoshito Yamashiro & Yoshito Yamashiro lab #CardiovascRes 2022
https://academic.oup.com/cardiovascres/advance-article/doi/10.1093/cvr/cvac190/6942105

How CD45 titrates the extent of mesenchymalization of #EndothelialCell?

CD45 stabilizes VE-cad junction via fortifying integrin α11-SHARPIN complex

Very interesting data on Biomechanics of #EndMT 😋

What (beyond CD45) control partial-to-complete EndMT transition?🤓

Induced #EndothelialCell KLF4 KO in adult🐭->
Lung Inflammation+Fibrosis within 1 month😎

KLF4-KO EC has⏫KLF2 & #EndMT simultaneously!
Perturbed shear-sensing?
Link b/w⏫KLF2 & #EndMT?
EC secretome? So interesteing😆

Dr. Kishore Wary lab @FrontCellDevBio 2022 @KishoreWary
https://www.frontiersin.org/articles/10.3389/fcell.2022.1003028/full

Evidence on #EndMT in human #MultipleSclerosis

Inflammation-induced endothelial to mesenchymal transition promotes brain endothelial cell dysfunction & occurs during multiple sclerosis pathophysiology

Dr. Helga de Vries lab Cell Death Dis 2019
https://www.nature.com/articles/s41419-018-1294-2

#EndothelialCell ARF6 GTPase links IL-1β inflammation with TGFβ/BMP-SMAD1/5 #EndMT

EC Arf6 KO in🐭#EAE->
⏫#BBB #BloodSpinalCordBarrier
⏬T cell infiltration & demyelination
w/o altering normal immunity

Dr. Shannon Odelberg, Weiquan Zhu labs Neuron 2022
https://www.sciencedirect.com/science/article/pii/S0896627322006602