What is a simple, quick & dirty method to induce Ca2+ release from the ER in a common cell-line like HEK293T?
I'm looking for something to be a positive control for Ca2+ release, and it needs to be a method that will not require cloning or purchasing an expensive drug.

#EndoplasmicReticulum

Last updated 2 years ago

Jorge Ferrer · @JorgeFerrer
48 followers · 44 posts · Server genomic.social

RT @EmboMolMed
Infancy-onset caused by de-regulated AMPylation of the human chaperone BiP

By D. Ron, E. De Franco & colleagues @Cambridge_Uni @UniofExeter

🗞️Read : doi.org/10.15252/emmm.20221649

#diabetes #EndoplasmicReticulum #openaccess

Last updated 2 years ago

Jonny Nixon-Abell · @AbellJonny
28 followers · 7 posts · Server mstdn.science

Okay / time!!
My interests revolve around systems of subcellular organisation, from a and perspective. I’m particularly focussed on between organelles in funky cells like . I use a bunch of techniques ( / ) alongside in vitro reconstitution systems to study contact site biology. The is the best organelle…this is a hill I am prepared to die on.

#introduction #introductions #cellbiology #biophysics #membranecontactsites #neurons #microscopy #ElectronMicroscopy #SuperResolution #EndoplasmicReticulum

Last updated 2 years ago