I'm now the owner of an #Apple #iMac #G4 #sunflower 🌻

#retrocomputing increases

Caution must be exercised when using the G-quadruplex #G4 ligand PDS as an anticancer agent since it triggers genetic instability in neurons.

We show in the Journal of Biological Chemistry (JBC) how PDS damages neurons, notably via a massive modulation their transcriptional activity.

👉https://doi.org/10.1016/j.jbc.2023.105157

New nanomedicine results!

When G-quadruplex #G4/hemin complexes (GH) are combined with nanoparticles (UMOF), the resulting UMGH kills hypoxic cancer cells thanks to a new, in situ generated photodynamic therapy #PDT.

Indeed, GH convert hydrogen peroxide (H2O2) in O2 (G4-DNAzyme) in situ, which is then converted into reactive oxygen species (ROS) thanks to the UMOF.

👉 Now in Adv. Healthc. Mater. : https://doi.org/10.1002/adhm.202300561

Our new study in @iScience (gold OA) compares the ability of the small molecules TASQs (https://doi.org/10.1021/acs.accounts.2c00757) and the antibody BG4 to isolate G-quadruplex-DNA #G4 by affinity precipitation, in their respective protocols G4DP-seq and BG4-DNA-IP-seq
👉https://doi.org/10.1016/j.isci.2023.106846

A new role for BLM helicase!

BLM is known to bind to and unwind DNA G-quadruplex #G4. In this new Nucleic Acids Res. article, we show that it unwinds RNA G4 as well, thereby regulating stress granules (SG) dynamics.

👉 https://doi.org/10.1093/nar/gkad613

In collaboration with the group of E. Hornstein (WIS Rehovot, IS)

The biocatalytic properties of G-quadruplex #G4 (or GQ) when associated with hemin (G4-DNAzyme) depend on its topology but this parameter is difficult to control.

In ACS Catalysis, we reach this goal, embedding #G4 in a DNA junction-based suprastructure.

👉https://pubs.acs.org/doi/10.1021/acscatal.3c02818

In collaboration with J. Zun (Nanjing, PRC), P. Stadlbaeur (Brno, CZ) and J.-L. Mergny (Polytechnique, FR)

How to reliably identify RNA G-quadruplex #G4 in human cells?

By combining specific affinity precipitation (G4RP) and bioinformatic methods (G4RNA Screener), as shown by the group of Jean-Pierre Perreault

👉https://doi.org/10.1016/j.biochi.2023.07.014

In Biochimie, special issue "Last Year At Marienbad" with respect to the 8th international meeting on G4s that took place.. last year at Marienbad CZ 🙂 (https://g4thering.com/home/).

Found a cheap #iMac #G4 #sunflower, in working order and located near my parents' house, where I'll be in a week.

I'm still on the fence and trying to talk myself into not buying it, but I don't have a lot of hope about that 😅

#apple #retrocomputing

Let the festivities begin!
PowerBook5,8 and PowerBook5,9 in fully working & almost mint condition :)
#Apple #PowerBook #G4 #Retrocomputing

For years, scientists invest massive efforts to make catalytic DNA 𝘳𝘦𝘢𝘭𝘭𝘺 catalytically active.

We are close to bringing this wish into reality, combining DNA #G4 and peptides into CPDzyme, a 𝙧𝙚𝙖𝙡𝙡𝙮 active artificial enzyme.

A great collaboration with Jun Zhou and his team (Nanjing Univ., PRC)

Now published in the J. Am. Chem. Soc.
👉https://doi.org/10.1021/jacs.2c11318

Curious about the relationship between this cute dog and a peculiar RNA structure named G-quadruplex #G4?
👉 Pug versus pUG fold!

Have a look to a spotlight now published in Molecular Cell #MolCell covering an article published in Nat. Struct. Mol. Biol. #NatStructMolBiol
by Kennedy, Butcher et al. (https://doi.org/10.1038/s41594-022-00854-z) in

👉 https://authors.elsevier.com/a/1gX1g3vVUPK9mQ
(free download for 50 days)

👉 https://doi.org/10.1016/j.molcel.2022.12.010

https://youtu.be/69rOhh2dpWk
I miss this series deeply, Cinematech needs a remake.
#tv #gaming #G4

Happy to be involved in the development of rG4detector, a neuronal network that uncovers RNA G-quadruplex #G4 in cancer cells and their correlation with stress granules

With my colleagues at Weizmann Institute of Science, IS

👉 in Nucleic Acids Res.
https://doi.org/10.1093/nar/gkac950

This is how a G4 CPU (out of a PowerMac G4 MDD) does look like after being fried due to not being cooled. Notice the "burnt" area bottom left?

#Retrocomputing #PoweMac # PowerPC #G4

In this new @biorxivpreprint we clicked (Sharpless/Meldal’s CuAAC and @CarolynBertozzi's SPAAC 🤟#ChemNobel 🤟) biomimetic G-quadruplex #G4 ligands to see these non-canonical, four-stranded DNA/RNA structures in human cells and isolate them in vivo

👉https://doi.org/10.1101/2022.10.28.512542