Tetramerization of AMPA-receptors - almost no effect of TARPs. I start to wonder what they are for...solid work as ever from the Wollmuth lab.

https://www.jbc.org/article/S0021-9258(23)02255-X/fulltext

#GlutamateReceptors #IonChannels

Claudio Grosman explains how Brønsted catalyzed his wonderful work to understand nicotinic receptor #biophysics #IonChannels #SingleMoleculeBiophysics

Students patch on our mini setup under the watchful eye of Jelena Baranovic. #biophysics #ionchannels

Claudio Grosman looks on as the activations of a single nicotinic acetylcholine receptor #IonChannels stream across the oscilloscope. #biophysics.

The #Berlin Single Molecule #Biophysics Course is underway! Jelena Baranovic gets to the heart of single #ionchannels. Wonderful and humbling to meet our participants from all over the world and excited for the week ahead.

***UPDATE - apply before 31st May, last few places.

We will offer a single molecule course at HU Berlin in September (4th-8th). Come and learn single channels and single molecule TIRF!

The course will not be free... but bursaries will be available.

https://www.biologie.hu-berlin.de/en/groupsites/cellbp/berlin-smb-course/bsmbc

Please boost (or even retweet)!

#biophysics #ionchannels #fluorescence #singlechannels #electrophysiology #TIRFMicroscopy #Berlin

Intracellular NASPM blocks outward currents in GluA2-lacking AMPA receptors, with no relief at all from a large panel of auxiliary proteins. Important new tool for identifying AMPAR diversity at synapses. We need more ways to peel off the different types! Nice paper from Ian Coombs in the Cull-Candy-Farrant lab at UCL
https://elifesciences.org/articles/66765

#ionchannels #glutamate #synapses #synapticdiversity #electrophysiology

Molecular dynamics simulations of the dimer of AMPA-type glutamate receptor binding domains from DE Shaw and colleagues. Seems to confirm some of our earlier functional work on desensitization (including one of the first mutants we published in 2012!), and also the transient encounters of glutamate on its way to the binding site. 190 µs of simulations!
https://www.sciencedirect.com/science/article/pii/S0969212623000965

#ionchannels #glutamate #MolecularDynamics #electrophysiology #receptors

RT @nupharm1
Zebrafish were used to determine the pathogenicity of KCNQ1 variants of uncertain significance. I'm tempted to say this sounds 'fishy' but it's actually a really cool, in vivo screening system! https://doi.org/10.1016/j.yjmcc.2023.03.001 #ionchannels #LQTS #potassiumchannels

Monumental paper of unnatural amino acid (UAA) mutagenesis in calcium release channels from Isabella Derler and team! Light-activated calcium release in complexes without STIM1 and tons of insights to structure function.

https://www.nature.com/articles/s41467-023-36458-4

#Chapeau #IonChannels #Optogenetics #GeneticCodeExpansion

Ion permeation through trimeric P2X channels - lateral windows at the base of the channel influence ion selectivity.

More insights into the most enigmatic and confusing of ion channel pores from my old colleagues Mufeng Li and Kenton Swartz at NIH.

https://doi.org/10.7554/eLife.84796

#IonChannels #purinergic #P2X

Structures and analysis of three modulators that bind to #AMPAReceptor complexes including the gamma-8 subunit from Ingo Greger's lab.

Nice to see this work that was presented at the #GRC #IonChannels last year coming out, and nice to see proof of @remislp conversion to glutamate.

These compounds were found by functional screening. Considering the heterogeneity of TARP modulation that is now apparent, a strategy of rational design might never have found them.

https://www.nature.com/articles/s41467-023-37259-5

We will offer a single molecule course at HU Berlin in September (4th-8th). Come and learn single channels and single molecule TIRF!

The course will not be free... but bursaries will be available.

https://www.biologie.hu-berlin.de/en/groupsites/cellbp/berlin-smb-course/bsmbc

Please boost (or even retweet)!

#biophysics #ionchannels #fluorescence #singlechannels #electrophysiology #TIRFMicroscopy #Berlin

Structures of my old friend kainate receptor GluK2 with the no-longer AMPA-receptor specific anti-epileptic drug Perampanel bound, and a blocker of desensitisation. Sobolevsky lab.

https://www.cell.com/cell-reports/fulltext/S2211-1247(23)00135-3

#ionchannels #neurotransmission #epilepsy

Unexpected early evolution of acid sensing ion channels, before brains, from Tim Lynagh and collaborators.

https://doi.org/10.7554/eLife.81613

#ionchannels #receptors #evolution #bilateran

Compare with simulations on AMPARs (using the multisite model) where we readily see Ca2+ permeation, and confirmed water statistics and polarisation effects with QM/MM.

In classical force fields, calcium doesn't move, it just gets stuck (see: https://www.nature.com/articles/s41467-020-14573-w). In contrast, the multisite model is accurate in energy and validated in hydration statistics.

https://chemrxiv.org/engage/chemrxiv/article-details/631f1665faf4a4165e0f0d5f

#biophysics #GlutamateReceptors #MolecularDynamics #dynion #Electrophysiology #ionchannels #calcium

Free link (50 days) to the final version of our paper about monovalent ion permeation in NaK mutants emulating glutamate receptor selectivity filters https://authors.elsevier.com/a/1gYpQ54HFRkZC

The preprint is free for the long term (and it's quite similar):

https://www.biorxiv.org/content/10.1101/2022.08.08.502946v2

#biophysics #ionchannels #selectivity #Crystallography #MolecularDynamics #Electrophysiology

Our article on the asymmetry of hybrid selectivity filters is online. Unfortunately access to this version is restricted. https://www.sciencedirect.com/science/article/abs/pii/S0022283623000268

But the preprint has been freely available on @biorxivpreprint for a while, and will continue to be.

https://www.biorxiv.org/content/10.1101/2022.08.08.502946v2

It's almost the same -figures in a different order, some explanations missing, no new data.

Congrats Sonja, Saeid and Sebastian!

#ionchannels #biophysics #physiology #Crystallography #MolecularDynamics #Electrophysiology

Please register for this wonderful symposium to mark the conclusion of the @dfg_public Dynion Forschergruppe. Fantastic line up of international speakers. Lots of opportunities for young scientists to present.

#IonChannels #physiology

https://www.uniklinikum-jena.de/dynion/en/Symposium.html

Grateful to the @dfg_public for funding this work at its outset through the TRR186 grant (to Peter Hegemann and me). #HUBerlin #CalciumSignaling #IonChannels