Happy to introduce our latest gizmo from the lab: pLSM !
https://www.biorxiv.org/content/10.1101/2023.05.31.543173v1

A big shoutout to our brilliant grad student, Yannan Chen, who led this work.

In short, pLSM is a plug-and-play, cost-effective and easy-to-scale light sheet microscopy platform. More than 20 times cheaper, with imaging quality comparable to high-end systems.

https://github.com/tomerlab/pLSM-Control

#LSFM #preprint #biorxiv #lightsheet #dopamine

Join us TOMORROW at the @monash Advanced Microscopy Webinar

27th April 2023 🔥 7am UK time (yes!)
"How to Quantify a Zebrafish: Research Software for Image-Driven Biomedical Studies"
All things #zebrafish, #microscopy, &#data.

DM for details.

#SPIM #LSFM #zebrafish #BIAnalysis

RT @KuglerElisabeth
Want to hear about state-of-the-art imaging and image analysis approaches?

Join us at the @monash Advanced Microscopy Webinar
27th April 2023 🔥 7am UK time (yes!)

DM for details.

#SPIM #LSFM #zebrafish #BIAnalysis

Want to hear about state-of-the-art imaging and image analysis approaches?

Join us at the @monash Advanced Microscopy Webinar
27th April 2023 🔥 7am UK time (yes!)

DM for details.

#SPIM #LSFM #zebrafish #BIAnalysis

Want to hear about state-of-the-art biomedical image analysis approaches to visualize, quantify,
and understand the biological processes?

Join us at the @monash Advanced Microscopy Webinar
27th April 2023 🔥 7am UK time (yes!)

DM for details.

#SPIM #LSFM #zebrafish #BIAnalysis

The protocol provides the step-by-step experimental methods for our @eLife paper "3D cell neighbour dynamics in growing pseudostratified epithelia" https://elifesciences.org/articles/68135.

See also our Tweetorial:
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RT @DagmarIber
How many neighbours does an epithelial cell have? And how stable are these interactions over time? #epithelialcell #topology #LSFM #SPIM

Check out our latest pre-print: https://www.biorxiv.org/content/10.…
https://twitter.com/DagmarIber/status/1368806675495452672

Who doesn't love a bit of morphogenesis and light sheet?🔬

Registration is needed.

#lightSheet #LSFM #SPIM #evoDevo #morphogenesis #drosophila
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RT @luxendo
We are incredibly excited to welcome Dr. Matteo Rauzi as webinar speaker.

Studying #Morphogenetic Waves with Photo Manipulation Coupled to Multi-View Light‑Sheet Microscopy

22-03-2023 — 10AM PDT | 1PM EDT
Sign-Up:
https://buff.ly/41pnMcz

#evoDevo #science #LSFM
https://twitter.com/luxendo/status/1631630005062057985

One pain a day. One acronym a day. #lightsheet #SPIM #LSFM and yes 160th!
Thanks to a keen follower @MicroEos
here is the 160Th and what an acronym MUSI (microscopy with ultraviolet single-plane illumination)...they missed MUSIC by one Correlative...
https://www.biorxiv.org/content/10.1101/2023.01.02.522477v1.full

One pain a day. One acronym a day. #lightsheet #SPIM #LSFM THE END
And to avoid the withdrawal syndrom I will give a few doses in the coming days...You are welcome to use them in your talks!

ne pain a day. One acronym a day. #lightsheet #SPIM #LSFM THE END
Thanks to @glaevsky
and @LSFMMBL
I started this horrrible 🧵 and I will stop there and slowly upload everything on @github
for people to play with and add more...My last count is a 159...yes 159!

One pain a day. One acronym a day. #lightsheet #SPIM #LSFM COUNTDOWN TO 2024...
2022 - Flexi-SPIM (So good!), OPSIM (OPM with SIM), IDDR-SPIM (Isotropic Divide stages-to-process (IDSP) double-ring (DR) modulation-SPIM), NIR-II-ASLM (Near-InfraRed II-ASLM)...

RT @HecksherS
Adult rat brain stained for TH and imaged using light sheet microscopy!
https://www.gubra.dk/landing-cns/

#parkinsonsdisease #neurodegeneration #neuroscience #CNS #preclinical #amazingbrain #lsfm

One pain a day. One acronym a day. #lightsheet #SPIM #LSFM COUNTDOWN TO 2024...
2020 – dOPM (Dual-view OPM), LLSM (Live-cell Lattice light-Sheet Microscopy) AKA La Lorraine de Saint Mande, LLSDM (Lattice Light-Sheet Difference Microscopy), compactLSFM (No comment!)...

One pain a day. One acronym a day. #lightsheet #SPIM #LSFM COUNTDOWN TO 2024...
2019 – AFM-LS (Atomic Force Microscopy-Light Sheet), AO-LSFM (Adaptive Optics LSFM), DC-APOM (Digital Confocal-Axial Plane Optical Microscopy), DOPM (Dual-view OPM)..AKA Dept of Prison Management..

One pain a day. One acronym a day. #lightsheet #SPIM #LSFM 2021
Reaching 137 acronyms and 2022 is next! But let's get some rest for the final stretch! See you in the New Year with more funky one such as 4D CMLS!

One pain a day. One acronym a day. #lightsheet #SPIM #LSFM 2021
How long can you make it! 3D LSRM cubic spline algorithm-based depth-dependent fluorescence-free three-dimensional light-sheet super-resolution microscopy
https://pubs.rsc.org/en/content/articlelanding/2021/an/d1an00852h

One pain a day. One acronym a day. #lightsheet #SPIM #LSFM back to 2021
Nothing beat a bit of BLIM bi-directional light-sheet illumination microscope before the week-end...looks like a T-SPIM to me...
https://www.sciencedirect.com/science/article/pii/S0006291X21005738

One pain a day. One acronym a day. #lightsheet #SPIM #LSFM 2021
Today double acronym game with OT-LSM and OTAS-LSM but not OTO-LSM too bad...Open-top light-sheet microscopy and so open-top axially swept LSM... afetr all why not...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086456/

One pain a day. One acronym a day. #lightsheet #SPIM #LSFM Catching up...
As I explore the forever expanding realm of acronyms I fousd some more so here are a few left overs...DSLS (2018) Diffuse light-sheet.. and sorry for the miss
https://pubmed.ncbi.nlm.nih.gov/29920963/

One pain a day. One acronym a day. #lightsheet #SPIM #LSFM 2021
you can LIC it if you want! by Partha Pratim Mondal. Light sheet imaging cytometry part ofthe iLIFE family for obviously integrated light-sheet imaging and flow-based enquiry...

https://aip.scitation.org/doi/full/10.10