#Autophagy in🐭#LimbIschemia 1/2

AMPK or Sestrin as potential autophagic regulators

Dr. Mattia Scalabrin & Scott Bowen lab AJP CellPhys 2022
https://journals.physiology.org/doi/abs/10.1152/ajpcell.00174.2022

Which tissue does autophagy occur in (likely #SkeletalMuscle based on Western blot)? And its functional impacts?

#Autophagy in🐭#LimbIschemia 2/2

Evidence on autophagy induced by ischemia/#Hypoxia in #EndothelialCell, and its stimulatory effects on #Angiogenesis

Dr. Joo-Won Jeong lab Cell Death Dis 2020
https://www.nature.com/articles/s41419-020-02849-4

Apold1, a #EndothelialCell-enriched gene, in pathological #Angiogenesis
#LimbIschemia ⏬Reperfusion (3 dpi🤓)-> An arteriogenic defect?
#Stroke ⏫border zone vascularization at day 21 but not day 7
Tumor ⏫Nonproductive angiogenesis-> more non-perfusable endothelial channels

Apold1 KO⏬EC Proliferation-How😁

@bohaceklab & Katrien de Bock bioRxiv 2022
https://www.biorxiv.org/content/10.1101/2022.12.02.518829v1

Also very interesting, Apold1 KO->⏫KLF2 & KLF4 in EC

Apold1 is recently identified as an ascending aortic EC-enriched gene (vs descending aorta/carotid artery)
Dr. Thomas Quertermous lab bioXRiv 2022
https://www.biorxiv.org/content/10.1101/2022.05.18.492517v1.full

Are Apold1 KO mice ready for #Atherosclerosis/#Aneurysm?

Cell Encapsulator made as PLLA/PLGA (50:50) electrospun porous (750 nm) scaffold

⏫👤Cardiac mesenchymal cell survival (1 mo in vivo) & #Exosome secretion

Implanted locally->⏫🐭#Limbischemia & #MyocardialInfarction

Dr. David Lundy lab J Controlled Release 2022
https://www.sciencedirect.com/science/article/pii/S0168365922007350