Multi-agent-based computational modeling of #Lumenogenesis from a single proliferating cell

High Matrix density & dynamic viscosity->
Formation of multiluminal structure instead of a single cyst lumen

This is a very easy-to-read computational modeling paper (minimal math requirement😁)
Cell cycle/polarity/secretion
Cell-cell (attractive+repulsive) + cell-fluid interaction (repulsive) + cell-matrix interactions
Geometry & Biomechanics (but not biochemical property of matrix)

Dr. José Manuel García-Aznar lab Eng Comput 2022
https://link.springer.com/article/10.1007/s00366-022-01654-1

Kidney epithelial #Tubuloid

CD24+ epithelial progenitor-derived
4-phase protocol, Matrigel +Wnt3a/RSPO1, grow > 6 mo
CRISPR modeling #ADPKD, Cyst size responsive to #Tolvaptan

Super-impressive single-cell or single-nuclei RNAseq analyses to dissect the spatiotemporal molecular/cellular dynamics of this cool renal tubuloid, laying a critical foundation to enhance its clinical utilities👍

Wonderful #Lumenogenesis of CD24+ epithelial progenitor-derived nephrospheres, day 7 in Matrigel🤠

Dr. Rafael Kramann lab @NatureGenet 2022 @jackxuyx @KuppeChristoph @rkramann
https://www.nature.com/articles/s41588-022-01202-z

Pancreatic Rab11a/Rab11b dKO->
Aberrant polarized transport of apical/basolateral proteins->
AMIS supernumerary->
Defects in ductal #Lumenogenesis; Acinar morphogenesis; Pancreatic cell lineage dysregulation

Highly intriguing, PancRab11KO->⏬Sox9+ bipotent progenitor & #Islet mass-> #GlucoseIntolerance in suvived adult🐭

Lumenized epithelial paracrine or endocrine-autonomous defect?😆

No baseline change in fast glucose. Insulin/glucagon? Adult islet histology-Amyloid degeneration?

The branched, lumenized pancreatic duct is fragmented into disconnected Microlumens + enlarged Cyst in PancRab11KO🐭

Can't help but think Rab35 in anisotropic Lumenogenesis of liver #BileCanaliculi

Is there a primary hydro-osmotic defect here?😋

Still, what happens to pancreatic vascular morphogenesis in Panc-Rab11 KO🐭?(Surely, epithelia & endothelia are coupled🤪)

More importantly, now it's the magic timing if you also want to have such amazing stories

Cleaver lab is recruiting TWO postdocs

Dr. Ondine Cleaver lab @ondinecleaver2022 bioXRiv 2022
https://www.biorxiv.org/content/10.1101/2022.11.17.516976v1