Better late than never for #MicroscopyMonday!
Here is a z-stack (every focal plane moving vertically through a sample) of actin polymer-stained Naegleria amoebae, followed by a maximum intensity projection (the brightest xy point of all the z positions). The darker colors here represent places where there is more actin polymer.

Happy #MicroscopyMonday!
Here's a movie from the "Titus Takeover" week, where Meg Titus and several million Dictyostelium cells visited the Fritz-Laylin Lab!
Here are 2 movies (1st at 4x, 2nd at 10x) of Dicty cells streaming to form mounds during overnight starvation.

Spent the afternoon in the #microscopy room, seeking meaning in patterns of multi-coloured dots again…sadly, falling prey to some wishful thinking along the way 🙃

Are you curious about what these pink and green dots are? Come to chat to @clathrin and I at the upcoming @J_Cell_Sci ‘Imaging Cell Dynamics’ meeting in Lisbon, where I will be presenting a poster!

#MicroscopyMonday #CellBiology #celldyn2023

Happy #MicroscopyMonday!
Here is a projection of 10 minutes of amoebae crawling on glass, so you can see their tracks. Not bad for a 4x objective and transmitted light!

LUT: CMOcean, curl

Happy #MicroscopyMonday!
Here's Naegleria gruberi as a dividing amoeba and as a flagellate

Happy #MicroscopyMonday!
Here is a ~very~ blebby amoeba making its way through a narrow channel.

Happy #MicroscopyMonday!
Here's one from my PhD work:
This cell is infected with Enteropathogenic E. coli (EPEC). EPEC translocates its own receptor (Tir, labeled pink) which triggers actin assembly (green) intro protrusive pedestals.

Happy #microscopymonday!
Here are some mammalian cells infected with Listeria, stained for actin polymer. ☄️

Happy #MicroscopyMonday!
Here’s a collage I made a while back highlighting Naegleria’s actin (cyan) and microtubule (orange) cytoskeletons. Naegleria only use microtubules to build a mitotic spindle, and when they transiently transform into a flagellated cell type.

Happy #MicroscopyMonday!
Here is an amoeba crawling through a microchannel. Blue areas show where the cell is in close contact with the coverslip (imaged by IRM), and DIC is shown in grayscale.

Blood vessels of a developing #zebrafish.

Happy #MicroscopyMonday!

The vessels are about 10 micrometer in diameter. That's just a little bit bigger than blood cells ..

#science #microscopy #vasculature

Happy #MicroscopyMonday!
Here's a video of amoebae crawling around before and after the addition of a fluorescent membrane dye

A little late for #MicroscopyMonday, but I still wanted to share this movie of Naegleria's contractile vacuoles that I just finished color coding!

Here's a cute submission for #MicroscopyMonday ❤️ Shown is a primary cilium from an IMCD3 cell labeled with acetylated alpha-tubulin in red. Smoothened, the GPCR that is activated downstream of Sonic Hedgehog signals, is shown in green. To make the heart, the cilium image was duplicated and flipped using Photoshop. Have a great week!

Happy #MicroscopyMonday! Here is a crystal that formed in dried stabilized bleach.

Happy #MicroscopyMonday!
Here are some very ruffled amoebae, stained for actin polymer
LUT: mpl-magma

"It's #MicroscopyMonday, so let's take a dive under the  and check out muscle circulation of the adult amphipod (Parhyale hawaiensis), sometimes called the "sand flea." Credit: Longhua Guo. Taken during an MBL Embryology course, imaged on

@zeiss_micro

LSM 780."

https://twitter.com/MBLScience/status/1597296540179128322?s=20&t=uk8i9cEU32eaiG7voAgq8g

For #MicroscopyMonday a Wnt1cre; tdtomato fetal mouse identifying dorsal root ganglia & craniofacial nerves and cartilages #neuralcrest #DevBio . Taken with a Zeiss Acmxio Zoom stereomicroscope.

It's Monday, so let's cheer us up with some pretty #MicroscopyMonday!

#SPYprobes

RT @AnhHLe2702
This cell has particularly huge mitochondria. You can see their cristae (you can see the zoom-in of the square area), which is the first time for me to ever see them so clearly! :O A belated #MicroscopyMonday #Fluorescence