Anyone using minKNOW v 22.10.10? I've got a base caller problem... Guppy not recognised (Its the GPU version). Other people on the release notes page reporting the same.
* Can I install standalone Guppy and point minKNOW at it somehow?
* Or should it run if i disable live basecalling?
Tech support haven't answered me yet but I assume it's a proper problem if multiple users affected 😅
#ONT #nanopore #MinION #sequencing
#ONT #nanopore #MinION #sequencing
Yes... everyone bow down to ME...
WHOA! Okay, um... my other minions need me over here... so I'll be back... kaythanksbye...
When you expect to be worshipped, but it wasn't QUITE what you expected...
#cat #cats #Caturday #Saturday #worship #minion #minions #bow #bowdown #funny #humor #soundon #soundup #catsofmastodon #mastodoncats
#cat #cats #caturday #saturday #worship #MinION #Minions #bow #bowdown #funny #humor #SOUNDON #soundup #catsofmastodon #mastodoncats
RT @RudolfMeier15
Now out in Molecular Ecology: Metabarcoding of dissolved feces from flies baited with fish detects endangered mammal and bird species. Roadside sampling and sequencing with #MinION or #Illumina is sufficient. Network analysis reveals species interactions. https://doi.org/10.1111/mec.16767
Sequencing #Illumina libraries at high accuracy on the ONT #MinION using R2C2 http://m.genome.cshlp.org/content/early/2022/11/08/gr.277031.122.abstract
As a way of extending an #introduction to the #science I have most fun doing, I'll do a few toots on different areas of our #research.
In 2016, we realised we could perhaps take #DNA #sequencing to the field, to generate #microbiomes & #metagenomes in real time. Some of our lessons from #nanopore field #genomics are here: https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.001220
It's meant we've sequenced on glaciers, in glaciers, coal mines, drill sites, and for #scicomm. Here's a #MinION being run for RI Christmas Lectures
#introduction #Science #research #DNA #sequencing #microbiomes #metagenomes #nanopore #genomics #scicomm #MinION
@ChristosArgyrop Oh, interesting! I've been so focused on getting *more* reads (we're waiting on our P2 right now) that I probably haven't thought enough about flongles for method development. Right now I just use mostly-spent #MinION cells (<300 pores), but at ~$65 a #flongle we should clearly be doing that. Nice tip!
DNA RNA Sequencing
MinION sequencing | Oxford Nanopore Technologies
https://nanoporetech.com/products/minion