Cool line up for this brain #organoid course at CRG-Barcelona, but too bad they could not get Dr. Michael Hfuhruhurr.

https://www.crg.eu/en/event/coursescrg-brain-organoids-advanced-hands-course-2023

Prostate cancer #organoid models.

"In summary, the availability of a tractable culture system for the in vitro growth of a variety of progenitor and differentiated prostate epithelial cells has facilitated the identification and molecular characterization of normal and transformed subpopulations as well as transient changes that occur in these subpopulations (46), deepening our understanding of the mechanisms of transformation underpinning prostate cancer."

https://www.jci.org/articles/view/169616

Tools #Organoid #CRISPR #preprint

"However, to date, the editing efficiency of organoids with the use of non-viral electroporation methods has been only up to 30%, with implications for the subsequent need for selection including including turnaround time and exhaustion or adaptation of the organoid population. Here, we describe an efficient method of intestinal organoid editing using a Ribonucleoprotein CRISPR-based approach."

https://www.biorxiv.org/content/10.1101/2023.03.31.535108v1

Referenced link: https://medicalxpress.com/news/2023-02-organoid-non-alcoholic-fatty-liver-disease.html
Discuss on https://discu.eu/q/https://medicalxpress.com/news/2023-02-organoid-non-alcoholic-fatty-liver-disease.html

Originally posted by Phys.org / @physorg_com: http://nitter.platypush.tech/medical_xpress/status/1628786789744795653#m

RT by @physorg_com: Novel #organoid models to study non-alcoholic #fattyliverdisease @_Hubrecht @NatureBiotech https://medicalxpress.com/news/2023-02-organoid-non-alcoholic-fatty-liver-disease.html

Blood Vessel Organoids for Development & Disease

Self-organizing capillary blood vessel #Organoid #EndothelialCell+#Pericyte (+Astrocyte for #BBB)

Transplant to gain vascular hierarchy

Organotypism Through Genetic Drivers

Dr. Josef Penninger lab Circ Res 2023
https://www.ahajournals.org/doi/10.1161/CIRCRESAHA.122.321768

We are always interested in students for bachelor and master theses. The topics range from #bioinformatics, #bioanalytic|s to medical #pharmacological topics. For example, we are investigating whether #CBD has a DNA damaging effect (DSB, DNA double strand break). We would be happy to find someone who wants to work with #brain #organoids.

#DSB #dnadamage #bachelorthesis #masterthesis #fedijob #organoid

Brain organoids Summer School

From theory to practice, hands-on course with experts!

The main objective of the School is to train the participants in the generation and processing of brain organoids and inspire the elaboration of new collaborations/projects through the close interactions between the speakers (mentors) and the students.

#Neuroscience #Brain #Neurology #Organoid #BrainOrganoid

https://sites.google.com/achucarro.org/bbioh/brain-organoids-summer-school?pli=1

In vitro systems to study endothelial/pericyte cell interactions

Hydrogel
Collgen/Matrigel/PEG

#Microfluidic #VesselOnAChip

Engineering microvessels

Blood vessel #Organoid
BBB organoid

Dr. Emily Warren & Sharon Gerecht Vascular Biology 2023
https://vb.bioscientifica.com/view/journals/vb/aop/vb-22-0021/vb-22-0021.xml

Thanks @SFB_TRR225 and @Biofabwue for hosting me! It has been a beautiful opportunity to discuss new ideas and collaboration opportunities @ISBioFab @RMUtrecht @VET_UniUtrecht @UMCU_Intl
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RT @SFB_TRR225
We @SFB_TRR225 and @Biofabwue are excited to host a seminar by Riccardo Levato (@RicLevato) of University Medical Center Utrecht. Thank you for the fascinating talk on "Advanced #Organoid Assemblies and Cell-based Architectures in #B…
https://twitter.com/SFB_TRR225/status/1616388062799347714

OCTOPUS, ein optimiertes Gerät zur Züchtung von Miniorganen in einer Schale (Bionity)
Ingenieure entwickeln ein Gerät, mit dem sich Darmorganoide zu einem noch nie dagewesenen Reifegrad entwickeln können - unter Verwendung patienteneigener Zellen
#Organoid #Zellkultur #Biotech
@Albert
Aktueller Ticker von: #dasneueuniversum
(BAND 120 übrigens jetzt im Buchhandel und unter https://dasneueuniversum.com
https://www.bionity.com/de/news/1179121/octopus-ein-optimiertes-geraet-zur-zuechtung-von-miniorganen-in-einer-schale.html?WT.mc_id=ca0067

OCTOPUS, ein optimiertes Gerät zur Züchtung von Miniorganen in einer Schale (Bionity)
Ingenieure entwickeln ein Gerät, mit dem sich Darmorganoide zu einem noch nie dagewesenen Reifegrad entwickeln können - unter Verwendung patienteneigener Zellen
#Organoid #Zellkultur #Biotech
@Albert
Aktueller Ticker von: #dasneueuniversum
(BAND 120 übrigens jetzt im Buchhandel und unter https://dasneueuniversum.com
https://www.bionity.com/de/news/1179121/octopus-ein-optimiertes-geraet-zur-zuechtung-von-miniorganen-in-einer-schale.html?WT.mc_id=ca0067

Vascularization improves function performance of #hiPSC-β Islet #Organoid 🤠

Ca2+ as a surrogate for insulin secretion to high throughput screening

A role of #Angiocrine #BMP4 signaling & #BasementMembrane

Dr. Maike Sander lab bioRxiv 2022
https://www.biorxiv.org/content/10.1101/2022.10.28.513298v1.full

Skeletal #muscle is a type of tissue that makes up a large part of the human body. It is made up of many different cells that are able to contract and move. Skeletal muscle has the ability to #repair itself when it is damaged due to #aging, exercise, or diseases like #MuscularDystrophy. A small group of cells called #SatelliteCell s help with the repair process. Scientists have been trying to create models to study how #Skeletalmuscle develops and regenerates. Recently, they have been using human pluripotent #StemCell to create 3D models of skeletal muscle tissue. However, these models have not been able to recreate the full process of muscle regeneration. In this research paper, the authors introduce a new method of using human pluripotent stem cells to create 3D models of skeletal muscle tissue that can retain the ability to repair itself.

Over the past decades, scientists have used #animalmodel to study #muscleregeneration, which is regulated by #stemcell s. These animal models have been very helpful in understanding the mechanisms of muscle #regeneration, but they don't always accurately reflect the same range of diseases that humans experience. Therefore, researchers have suggested creating reliable in vitro models using human muscle cells. ( #hPSC s) could be used to create 3D human skeletal muscle #organoid s ( #hSkMO s) that contain sustainable #stemcell and distinct myofibers with the same proteins and structure as adult muscles. Previous approaches to skeletal muscle differentiation have been developed using 2D #culture systems, but these lack the natural environment and #StemCell niche that are necessary to model adult #myogenesis and muscle #regeneration.

#Stemcell s ( #SC s) can be used to repair damaged muscle tissue. They explain that SCs can be activated in response to muscle injuries and that other #cell types can contribute to the process of #myogenesis. The author then goes on to explain that #cytokine s, such as IL-4, can influence the #InflammatorySystem and promote SCs differentiation, which helps with muscle regeneration. While #organoid s generated from #hPSC s have potential, they do not fully replicate the in vivo native microenvironment. To address this, treat the #hSkMO s with extrinsic #cytokine s to promote #muscle #regeneration . #hSkMO s might then be used to study aspects of human muscle #biology and to identify novel #therapeutic candidates for muscle-wasting disorders.

To create a 3D structure of muscle tissue. They used #WNT activator and #BMP inhibitors at the beginning of the differentiation process to induce paraxial #mesodermal #cell s. They then added #FGF2 to the Matrigel to promote the 3D structure. #HGF and IGF1 were added later to accelerate the #myogenic specification and further #myofiber differentiation. They optimized the timing of the Matrigel embedding to day seven. After this, they observed #neuralcell s and withdrew FGF2 to focus on muscle tissue development. They then prolonged the HGF and IGF1 treatment to propagate #myogenic #progenitor s. They found that 62% of the #tissue was #skeletalmuscle tissue and that it contained PAX7+ #myogenic #stem / #progenitor cells, MYOD+ activated/committed #myoblast s, and MYOG+ #myocyte s. They also found that 31% of PAX7+/Ki67− and 29% of MYOD−/PAX7+ non-dividing quiescent SCs were present in the mature #hSkMO s. This indicates that the #hSkMO s were able to effectively recreate #embryo nic #myogenesis and have regenerative potential. Future studies using #singlecell #RNA sequencing may be necessary to further characterize the different types of cells in #hSkMO s.

The stepwise process to generate human skeletal muscle organoid s (hSkMOs) from human pluripotent stem cells (hPSCs)

The process begins with dissociating #hPSC s into #singlecell s and allowing them to form #embryoid bodies ( #EB s) in low-attachment V-shaped 96-well plates. Then, paraxial #mesodermal differentiation is promoted with #WNT activation, BMP inhibition, and FGF2 signaling. The expression of pluripotency markers OCT4 and NANOG decreases, and the expression of #mesoderm markers Brachyury, T-Box transcription factor 6 (TBX6), and mesogenin 1 (MSGN1) increases. To further characterize paraxial #mesoderm al differentiation, TBX6 is #immunostain ed. After paraxial #mesodermal induction, the #organoid s are embedded with growth factor-reduced Matrigel and transferred to a six-well plate on an orbital shaker. Growth factors are then added to the #myogenic specification media, and #hSkMO s are cultured until the day of analysis. The orbital shaker improves the viability, survival, and differentiation of hSkMOs by increasing the penetration rate of oxygen and nutrients into the core area of hSkMOs. The #hSkMOs gradually grow to more than 1.5 mm in diameter by day 60, appearing round-shaped, uniformly sized, and having relatively homogenous morphology. PAX3 and PAX7 are #myogenic progenitor markers, and their expression is verified by qRT-PCR and #cryo sections. The #myogenic cells appear as clusters, and approximately 9% of PAX7+ cells are double-positive for Ki67 at day 30, demonstrating that proliferating cells are #myogenic #progenitor s in hSkMOs. This indicates that the in vitro #hSkMO #culturesystem is able to recapitulate the features of embryonic skeletal #muscle development.

The different types of #SkeletalMuscle stem/progenitor cells that are involved in myogenesis, the process of muscle formation.

The researchers used qRT-PCR analysis and #immunohistochemistry to identify and characterize the different types of cells. They found that PAX3 and PAX7 (SC markers) were the major population during the early stage of #myogenesis, and that MYOD (proliferating and activated SC marker) and MYOG (differentiated myocyte marker) increased over time. They also observed that MYOD−/PAX7+, MYOD+/PAX7+, and MYOD+/Ki67+ cells accounted for 29%, 6%, and 8% of the putative quiescent, activated, and proliferating #SC s, respectively. MYOD+/PAX7− cells constituted 39% of differentiating myoblasts, and MYOG−/PAX7+ cells constituted 23% of putative quiescent SCs. MYOG+/PAX7− cells accounted for 30% of differentiated #myocyte s, and 8% and 6% of the MYOG+ cells in #hSkMO s co-expressed PAX7 and Ki67, respectively. This data shows that the researchers were able to identify and characterize different types of skeletal muscle stem/progenitor cells during #myogenesis.

The text is discussing the results of a research study that used hSkMOs (human skeletal muscle #organoid s) to study the development of skeletal muscle #tissue. The study found that the #hSkMO s grew exponentially in size within two months, and the growth rate then steadily decreased. The researchers then used scanning electron microscopy (SEM) imaging and confocal microscopy to examine the cytoarchitecture of the hSkMOs. They found that the hSkMOs contained a large population of terminally differentiated #myogenic cells and a small population of preserved myogenic stem/progenitor cells. They also found that the hSkMOs contained a substantial proportion of TITIN+ muscle cells and MAP2-positive #neuron s. To further characterize the presence of sustainable stem cells within the mature hSkMOs, they quantified the amount of dormant stem cells by #confocal #microscopy imaging. The results showed that approximately 56%, 31%, and 5% of PAX7+/Ki67- putative dormant stem cells existed throughout the differentiation of hSkMOs at days 30, 70, and 130, respectively. This indicates that the hSkMOs contained mature skeletal muscle properties and had the potential for #regeneration .

The researchers wanted to see if the #hSkMO s (human #skeletal muscle #organoid s) had the ability to regenerate #muscle #tissue after damage. To test this, they treated the hSkMOs with a cardiotoxin (CTX) which is known to induce muscle inflammation and damage. They then observed a decrease in PAX7+ and MYOD+ cells in the hSkMOs. To further test the #regenerative potential of the #hSkMO s, they added interleukin-4 (IL-4) to the medium to promote #muscleregeneration. After 14 days, they observed a significant increase in MYOG+ myocytes in the CTX-injured hSkMOs with the treatment of IL-4 compared to the CTX-injured hSkMOs without the treatment. This suggests that the hSkMOs have the potential to regenerate muscle tissue after damage.

#explainpaper

Generation of Skeletal Muscle Organoids from Human Pluripotent Stem Cells to Model Myogenesis and Muscle Regeneration

Authors :

Min-Kyoung Shin , Jin Seok Bang , Jeoung Eun Lee , Hoang-Dai Tran , Genehong Park , Dong Ryul Lee and Junghyun Jo

Referenced link: https://medicalxpress.com/news/2022-07-organoid-production-breakthrough-disease-drug.html
Discuss on https://discu.eu/q/https://medicalxpress.com/news/2022-07-organoid-production-breakthrough-disease-drug.html

Originally posted by Phys.org / @physorg_com@twitter.com: https://twitter.com/medical_xpress/status/1552670237073592323#m

RT by @physorg_com: #Organoid production breakthrough to help accelerate disease and drug development research @ISSCR @nature https://linkinghub.elsevier.com/retrieve/pii/S2213671122003599 https://medicalxpress.com/news/2022-07-organoid-production-breakthrough-disease-drug.html