Hao Yin · @HaoYin
228 followers · 285 posts · Server qoto.org

Effects of on cell behavior & function

Cause:
ROS
DNA replication
mitosis
mechanical confinement

Direct dosage + secondary Effect:
Redox, bioenergetic, proteostasis, Osmosis
Cancer, Aging

Dr Rong Li & Jin Zhu Nature Reviews MCB 2023
nature.com/articles/s41580-021

====

Any evidence on in cardiovascular biology?

From Abraham Aviv lab @ATHjournal 2001

Age-dependent aneuploidy & telomere length of the human vascular endothelium
atherosclerosis-journal.com/ar

Tetrasomy Chr6 Chr16
Loss ChrY

Aneuploidy in cardiovascular diseases?🧐

====

Mice with protein BubR1 deficiency have a spectrum of vascular defects

Vascular BubR1 1/3

Aging-Associated Vascular Phenotype in Mutant Mice With Low Levels of BubR1

Dr. Jan van Deursen lab @StrokeAHA_ASA 2007
ahajournals.org/doi/10.1161/01

=======
Vascular BubR1 2/3

BubR1 Insufficiency Inhibits Through Impaired Vascular Proliferation in Mice

Dr. Takuya Matsumoto lab @atvbahajournals 2014
ahajournals.org/doi/10.1161/AT
====

Vascular BubR1 3/3

BubR1 insufficiency impairs in aging and in experimental critical limb ischemic mice

Dr. Takuya Matsumoto lab @JVascSurg 2018
jvascsurg.org/article/S0741-52

#NeointimalHyperplasia #aneuploidy #SpindleAssemblyCheckpoint #SmoothMuscleCell #angiogenesis

Last updated 2 years ago

Hao Yin · @HaoYin
228 followers · 283 posts · Server qoto.org

IL-11 as a therapeutic target for lung in

In Marfan🐭🫁, IL11-GFP reporter is active in Fibroblast but not alveolar epithelia

Dr. Stuart A Cook & Wei Wen Lim lab ATVB 2023
ahajournals.org/doi/10.1161/AT

Also don't miss the story on IL-11 as mediator of Mafan from Circ Res 2022

ahajournals.org/doi/10.1161/CI

#Aortopathy #fibrosis #emphysema #EndothelialCell #marfansyndrome #SmoothMuscleCell

Last updated 2 years ago

Hao Yin · @HaoYin
227 followers · 282 posts · Server qoto.org

Epigenetics

JMJD1C a histone demethylase
⏫p-STAT3->
HK2/PGK1/LDHA->
⏫PA Proliferation

Serum Lactate⏬by AAV2-shJMJD1C in🐭-PAH
Local effect on ?

Dr H Zhao lab Cell Death Discovery 2023
nature.com/articles/s41420-023

#SmoothMuscleCell #hypoxia #EndMT #PulmonaryArterialHypertension #glycolysis

Last updated 2 years ago

Hao Yin · @HaoYin
226 followers · 268 posts · Server qoto.org

BRD4 (acetyl lysine reader)-NIPBL (cohesin loading ) complex is critical to differentiation into

BRD4 depletion->⏬+loop extrusion

BRD4 in ?

Dr. Rajan Jain lab Nature Genet 2021
nature.com/articles/s41588-021

#neuralcrest #SmoothMuscleCell #aorticaneurysm #Cohesinopathy #GenomeFolding

Last updated 2 years ago

Hao Yin · @HaoYin
222 followers · 249 posts · Server qoto.org

Striking Pulmomary in
3 case+2 Ctrl
Thickened+Broken elastin in hyalinizing arterial wall😎

Thbs1-hi CD163+ non-canonical Monocyte in fatal COVID! To activate platelet only?🧐
Dr. Kiyoshi Hirahara & Toshinori Nakayama labs PNAS 2022
pnas.org/doi/10.1073/pnas.2203

Plasma MYL9 level corr. with COVID severity!

And look at these crazy MYL9/12+ thrombi in small pulmonary arteries😬

What's the role of MYL9, a contractile protein, in platelets?

This leads me into another wonderland (🐰hole)

Platelet-deposited MYL9/12 as Luminal scaffold guiding T-cell migration!?😈

Dr. Toshinori Nakayama lab Sci Immunology 2016
science.org/doi/10.1126/sciimm

Another intriguing paper on leukocyte vs arterial mural cell-derived MYL9/12 & 😆

Increased Myosin light chain 9 expression during Vasculitis

Dr. Motoko Kimura & Hiromichi Hamada labs Front Immunol 2022
frontiersin.org/articles/10.33

#vasculitis #KawasakiDisease #COVID19 #SmoothMuscleCell

Last updated 2 years ago

Hao Yin · @HaoYin
221 followers · 240 posts · Server qoto.org

De Novo pathogenic variant of miR-145-5p (het?😃) in

+mRNA & miR transcriptomes of patient skin fibroblast-/+TGFβ1 😋

Dr Mark Lindsay lab JCI 2023
@MarkELindsay
@markelindsay
jci.org/articles/view/166497

miR-145-5p is a master miRNA to maintain contractile phenotype

My favorite among the earliest miR-145 papers👇

Acquisition of the contractile phenotype by murine arterial SMCs depends on the Mir143/145 gene cluster

JCI 2009
jci.org/articles/view/38864
=====

miR-145
CARMN
SMILR
...

The (re-)arising of smooth muscle-enriched non-coding RNAs makes me really nostalgic for my PhD days & nights😸 in Calgary

#MultisystemSmoothMuscleDysfunctionSyndrome #raredisease #SmoothMuscleCell

Last updated 2 years ago

Hao Yin · @HaoYin
221 followers · 231 posts · Server qoto.org


- Synergy

EC Fbln4 KO exacerbates Aortopathy of SMC Fbln4 KO
-Aneurysm into Arch
-Descending Aortic tortuosity
-Postnatal aortic valve thickening/#EndMT

Dr. Yoshito Yamashiro and Hiromi Yanagisawa labs JAHA2022
ahajournals.org/doi/10.1161/JA

#ThoracicAorticAneurysm #SmoothMuscleCell #EndothelialCell

Last updated 2 years ago

Hao Yin · @HaoYin
217 followers · 204 posts · Server qoto.org

Arterial dissections: Common features and new perspectives

A comprehensive review covering diverse arterial beds

Biomechanics
+ plasticity
Genetics
TGFβ, ECM

Dr. Robert Graham lab Frontiers Cardiovascular Medicine 2022
frontiersin.org/articles/10.33

#EndothelialCell #SmoothMuscleCell

Last updated 2 years ago

Hao Yin · @HaoYin
211 followers · 192 posts · Server qoto.org

Spatial Atlas of human Lung

Spatially-resolved multi-omics-> -associated Lymphoid niche for IgA + T cells via CCL28/IL6/APRIL paracrine

5 locations, 13 patients

Systemic vs Pulmonary vascular trees

Immune recruiting venous NG2-ABCC9+ICAM1+ is abundant in CCL2/19/21 & CXCL12🤠

Love to dig into transcriptomes of systemic vs pulmonary & pericytes along the bronchial tree😋

A spatially resolved atlas of the human lung characterizes a gland-associated immune niche

Love this powerful molecular micro-anatomy for human Lung👍

A great starting point for my Holiday-specific continued biomedical education on Lung🎄

Dr. Sarah Teichmann & Kerstin Meyer lab Nature Genetics 2022
nature.com/articles/s41588-022

#AirwaySubmucosalGland #PlasmaCell #Pericyte #SmoothMuscleCell

Last updated 2 years ago

Hao Yin · @HaoYin
188 followers · 172 posts · Server qoto.org

An autosomal (Chr2) Myh11-CreERT2🐭model to enable -specific KO & lineage tracing in Both MALE & FEMALE!👍

Previous mouse Myh11-Cre transgenes are Y-linked

Dr. Gary Owens lab ATVB 2022
ahajournals.org/doi/10.1161/AT

#SmoothMuscleCell

Last updated 2 years ago

Hao Yin · @HaoYin
185 followers · 166 posts · Server qoto.org

IL-1β & Macrophage-like /SMC-derived

scRNAseq👤calcified (proximal vs core)->
Ligand-SMC receptor map + Mɸ-like SMC transcription network->
⏫IL1β-⏬STAT3

AAV9-Cas9-sgIL1β for🐭

Dr. Suxin Luo lab Comms Bio 2022
nature.com/articles/s42003-022

#FoamCell #atherosclerosis #SmoothMuscleCell

Last updated 2 years ago

Hao Yin · @HaoYin
185 followers · 159 posts · Server qoto.org

ApoE KO🐶♂️normal diet 18 mo
Multi-arterial ->
MI/Stroke/Gangrene

scRNAseq athero-prone vs -resistant arteries
+ - LTBP1+ myofibroblast transition in atherogenesis

Dr. X. Wang, YB Zhang, X Ji labs Aging Dis 2022
aginganddisease.org/EN/10.1433

#TenascinC #atherosclerosis #SmoothMuscleCell

Last updated 2 years ago

Hao Yin · @HaoYin
178 followers · 143 posts · Server qoto.org

SM22+ LRP1 KO->

Spontaneous Aneurysm

Baseline+Injured carotid arterial defect
(Intensive neointimal Elastin deposits!😈)

⏫Angiotensin+TGFβ signals

Dr. Alan Daugherty & Dudley Strickland labs @JCI_insight 2022
insight.jci.org/articles/view/

There is a long research history on LRP1 with SMC & arterial wall integrity, starting with this👇

LRP: Role in Vascular Wall Integrity and Protection from Atherosclerosis

Dr. Joachim Herz lab Science 2003
science.org/doi/10.1126/scienc

#SmoothMuscleCell #SuperiorMesentericArtery

Last updated 2 years ago

Hao Yin · @HaoYin
174 followers · 129 posts · Server qoto.org

Vascular dysfunction in

Vasculo-neuronal-inflammatory triad

Hypercontractile VSMC
⏬Clearance receptors for Aβ
Autoimmune-related SMC degeneration in

Dr. Genevieve Hayes lab @FrontNeurosci 2022
frontiersin.org/articles/10.33

#Neurodegeneration #alzheimersdisease #SmoothMuscleCell

Last updated 2 years ago

Hao Yin · @HaoYin
172 followers · 125 posts · Server qoto.org

Piezo1 promotes high Phosphate-induced & osteogenic differentiation of cultured👤aortic SMC (& culture🐭aorta)

How Piezo1-Ca2+ elevates expression of Runx2 & Transglutaminase 2?

Dr. Beatrix Dienes lab Front Physiol 2022
frontiersin.org/articles/10.33

#calcification #SmoothMuscleCell

Last updated 2 years ago

Hao Yin · @HaoYin
170 followers · 115 posts · Server qoto.org

An impressive panorama of Trimethylamine N-oxide/TMAO on👤aortic
⏫Traits of /Inflammation
Metabolic reprogramming
Kinome & transcriptional network

Intriguing role of PERK & mitoROS

In addition to Liver, Aorta (/#Pericyte/Fibroblast/Mɸ?) may🤔 also oxidize -derived trimethylamine/TMA into Trimethylamine N-oxide/TMAO

What is the local/Aortic vs circulating TMAO level?🧐

Wait for🐭tissue-specific KO of FMO3 (Oxidizing TMA)😆

Dr. Xiaofeng Yang lab JCI insight 2022
insight.jci.org/articles/view/

#EndothelialCell #TrainedImmunity #SmoothMuscleCell #gutmicrobiota

Last updated 2 years ago

Hao Yin · @HaoYin
158 followers · 101 posts · Server qoto.org

Itga8-CreERT2, a novel tamoxifen-inducible -specific Cre🐭

Selective for Vascular over Visceral (GI/Airway...) SMC🤠

Critical comparison (+Aorta transcriptomes) vs Myh11-CreERT2

Dr. Qing Lyu & Joseph Miano labs Nature Cardiovascular Research 2022
nature.com/articles/s44161-022

#SmoothMuscleCell

Last updated 2 years ago

Hao Yin · @HaoYin
156 followers · 98 posts · Server qoto.org

Chromatin landscape dynamics of in 🐭

Integrated scATACseq+scRNAseq+lineage tracing analysis reveals a protective role of ATF3 in atherogenic 3+ SMC

Dr. Ying Wang & Nicholas Leeper lab 2022
academic.oup.com/cardiovascres

#atherosclerosis #complement #CardiovascRes #SmoothMuscleCell

Last updated 2 years ago

Hao Yin · @HaoYin
152 followers · 91 posts · Server qoto.org

Airway forms Collagen III microniche⏫🫁 of
via DDR1-HSP90-Stat3 axis

In primary Collagen I-DDR1 axis⏫tumor invasiveness

Dr. Keith Syson Chan lab Nature Comms 2019
nature.com/articles/s41467-019

#SmoothMuscleCell #metastasis #MuscleInvasiveBladderCarcinoma #bladdercancer

Last updated 2 years ago

Hao Yin · @HaoYin
76 followers · 49 posts · Server qoto.org

Airway Inflammation

SMMHC-driven α7 transgene in ovalbumin♀️🐭->
⏬Airway leukocyte infiltration
⏬Pulmonary resistance (not compliance)
Re-balanced cytokine/chemokine production

Dr. Mariam Ba lab FASEB Bioadv 2022
faseb.onlinelibrary.wiley.com/

#integrin #SmoothMuscleCell #asthma

Last updated 2 years ago