Paper by Ueda et al "Rap1 organizes lymphocyte front-back polarity via RhoA signaling and talin1" in iScience @iScience
https://www.cell.com/iscience/fulltext/S2589-0042(23)01369-X
The role of talin1 in cell polarization, downstream of Rap1, seems to be independent of talin binding to integrins and of integrin adhesion
#Rap1 #talin #cytoskeleton #GEFs #GAPs

"Single-molecule magnetic tweezers to capture over a million individual transitions as a single talin protein unfolds and refolds under force in equilibrium. When observed at classically probed timescales, talin folds in an apparently uncomplicated two-state manner. As the sampling time extends from minutes to days, the underlying energy landscape exhibits gradually larger signatures of complexity, involving a finite number of well-defined rare conformations.[...]The physiological relevance of each distinct conformation can be connected to the binding of the cytoskeletal protein vinculin, suggesting an extra layer of complexity in talin-mediated mechanotransduction."
https://www.nature.com/articles/s41567-022-01808-4
#StructuralBiology #Talin #Vinculin #MechanoTransduction #MechanoBiology #Cytoskeleton