PastelBio · @pastelbio
179 followers · 2119 posts · Server mstdn.science

RT @byu_sam: . question about tools for TMT quant. Which tools incorporate the MS1 intensity into the quantitation from MS2-based reporter ions?
I know that @nesvilab does this with Fragger. But does anyone else?

#TeamMassSpec

Last updated 1 year ago

PastelBio · @pastelbio
179 followers · 2115 posts · Server mstdn.science

RT @byu_sam: . question about tools for TMT quant. Which tools incorporate the MS1 intensity into the quantitation from MS2-based reporter ions?
I know that @nesvilab does this with Fragger. But does anyone else?

#TeamMassSpec

Last updated 1 year ago

PastelBio · @pastelbio
178 followers · 1965 posts · Server mstdn.science

RT @DrLauraD22: Calling all students interested in . We have some excellent opportunities ⁦@WEHI_research⁩ proteomics facility. Please share! ⁦@FemalesInMS⁩ ⁦@PMV_Australia⁩ ⁦@LorneProteomics⁩ ⁦@hupo_org⁩

#TeamMassSpec #proteomics #phd

Last updated 1 year ago

PastelBio · @pastelbio
170 followers · 1788 posts · Server mstdn.science

RT @byu_sam: After reading this, I am struck that all the negative examples (where CV is wonky) are because of terrible data quality, like letting in peptides with no FDR filter.

But is CV a bad metric if I am doing proper FDR filtering?

#TeamMassSpec

Last updated 1 year ago

PastelBio · @pastelbio
170 followers · 1783 posts · Server mstdn.science

RT @: Assuming spray voltage is fine, what is the cause of the retrograde movement of the mobile along the emitter during ESI?

#TeamMassSpec #protoemics

Last updated 1 year ago

Sarah Hancock · @sarahehancock
148 followers · 3 posts · Server mstdn.science

Just in case anyone in is curious, the likely culprit of my 248.9 m/z ion from this thread: mstdn.science/@sarahehancock/1 is free cyanide reacting with the gold seals of the HPLC pump. I will have to try a few different brands of solvent to see if I can ameliorate this issue. More info also available here: community.agilent.com/technica.

#TeamMassSpec #metabolomics #lipidomics #massspectrometry #massspec

Last updated 1 year ago

PastelBio · @pastelbio
163 followers · 1657 posts · Server mstdn.science

RT @: R package for annotating and visualizing high res MALDI proteoform/peptide data (and more). Congrats to David Degnan who has been driving this effort. @J_ASMS

#TeamMassSpec

Last updated 1 year ago

Sarah Hancock · @sarahehancock
147 followers · 2 posts · Server mstdn.science

Hey , I'm having issues with a contaminant ion @ 248.9 m/z in -ve ion mode when running 90% ACN 10 mM AmOAc at pH 9. Found a thread on research gate suggesting it comes from the ACN but have tried two brands now with no joy. Has anyone else had this problem?

Research gate link: researchgate.net/post/What-is-

ACN brands tried: Fisher Sci Optima, Supelco LiChroSolv. LiChroSolv has a lot less of it than Fisher Sci.

#TeamMassSpec #metabolomics #lipidomics #massspectrometry #massspec

Last updated 1 year ago

PastelBio · @pastelbio
164 followers · 1618 posts · Server mstdn.science

RT @: Always good to see new tools available.Dear and , I am happy to share HowDirty, an R package that facilitates evaluating and reporting molecular contaminants in LC-MS experiments.
authorea.com/users/643346/arti

#massspec #proteomicscommunity #TeamMassSpec

Last updated 1 year ago

PastelBio · @pastelbio
164 followers · 1615 posts · Server mstdn.science

RT @: *Job alert* An amazing opportunity is available to join our fabulous team @WEHI_research proteomics facility. Applications close 28 August. Please retweet!

wehi.wd3.myworkdayjobs.com/en-

#TeamMassSpec

Last updated 1 year ago

PastelBio · @pastelbio
164 followers · 1600 posts · Server mstdn.science

RT @: Folks! We have created a survey to better understand users' experience with OpenMS. The survey is short, and we would love to hear your opinions on what's good and bad! Please share your thoughts

surveymonkey.de/r/9F6TMJL

#proteomics #metabolomics #lipidomics #TeamMassSpec #phd

Last updated 1 year ago

PastelBio · @pastelbio
159 followers · 1556 posts · Server mstdn.science

RT @: Deadline Sept 30th!
Call for papers @JProteomeRes Special Issue
"Women in and "
axial.acs.org/biology-and-biol
@FemalesInMS @AmerChemSociety

#proteomics #metabolomics #womeninSTEM #TeamMassSpec

Last updated 1 year ago

PastelBio · @pastelbio
158 followers · 1504 posts · Server mstdn.science

RT @: Anyone have advice on how they normalize TMT channel data when the samples are from a pulldown? Normalization methods assume that peptide abundances should be broadly equal across samples...

#TeamMassSpec #massspec #proteomics

Last updated 1 year ago

PastelBio · @pastelbio
155 followers · 1421 posts · Server mstdn.science

RT @: Last reminder: everyone attending check out your email to learn all about the YPIC activities! Join us for dinner on Sunday, workshops on Monday, breakfast on Tuesday or during any break at our booth! @UKBspr @EuPAProteomics

#bspreupa2023 #TeamMassSpec #conference

Last updated 1 year ago

PastelBio · @pastelbio
153 followers · 1367 posts · Server mstdn.science

RT @: Less than one week to go for
Check out the updated programme:
conferences.ncl.ac.uk/bspr-eup

@UKBspr @EU_YPIC @EuPAProteomics

#bspreupa2023 #TeamMassSpec #proteomics

Last updated 1 year ago

PastelBio · @pastelbio
147 followers · 1312 posts · Server mstdn.science

RT @: Announcing the 2023 @asmsnews Asilomar Conference on Computational Mass Spec.
Abstract submission, registration, lodging, and stipend application are all OPEN.
asms.org/conferences/asilomar-
RT @olgavitek @DavidFenyo

#TeamMassSpec

Last updated 1 year ago

PastelBio · @pastelbio
147 followers · 1295 posts · Server mstdn.science

RT @: Hey - question re: low sample stage-tip fractionation. Anyone have any experience with it? We have some POROS 20 R2 packing. How much (~mg?) do you load into a 200µL tip?

#TeamMassSpec #massspec #proteomics

Last updated 1 year ago

PastelBio · @pastelbio
147 followers · 1295 posts · Server mstdn.science

RT @: is just over 1.5 weeks away!
I'm getting more and more excited as the programme was just released! conferences.ncl.ac.uk/bspr-eup
@UKBspr @EU_YPIC @EuPAProteomics

#bspreupa2023 #TeamMassSpec #proteomics

Last updated 1 year ago

Dr Nick Birse · @njbirse
38 followers · 8 posts · Server mstdn.science

Hi - trying Mastodon again, last tried it in December 2022.

So, I'm a pretty new Lecturer in Mass Spectrometry at Queen's University Belfast.

My BSc was Pharmaceutical Chemistry at Dundee, followed by an MSc in Instrumental Analytical Science at Robert Gordon, and a PhD (plus postdoc) in mass spectrometry at Queen's.

If you do/like any of that lot (analytical chemistry and toxicology, really) then I'd love to chat.

#TeamMassSpec

Last updated 1 year ago

Dr Nick Birse · @njbirse
38 followers · 8 posts · Server mstdn.science

Welcome back to my Mastodon feed.

I'm now a Lecturer in Mass Spectrometry at Queen's University Belfast, and can still be found working on all sorts of mass spectrometry for food and environmental samples.

#TeamMassSpec

Last updated 1 year ago