@WhatisBiotechnology More on #telomerase 🧬 ⬇️

“Chemical Eye 👁️ on Motherly 👵 Love 💕” 👉 http://www.sitnews.us/MacDougall/050705_macdougall.html

'To study TERT deregulation and its downstream consequences, we generated a Tert mutant promoter mouse model via CRISPR/Cas9 engineering of the murine equivalent locus (Tert-123C>T) and crossed it with thyroid-specific BrafV600E-mutant mice.'

#Preprint #Telomeres #telomerase

https://www.biorxiv.org/content/10.1101/2023.01.24.525280v1?med=mas

A paper that I was not aware of in @embojournal that scientific editor @HVodermaier mentioned in the #telomerase independent immortalisation discussion on the latest #EMBOPodcast episode is the finding from Lackner, Karlseder et al in #EMBOJournal 2012 of a very interesting ALT system in #Celegans

🎧 to the episode
https://www.embo.org/podcasts/alternative-immortality/

Read the paper (#OpenAccess)
https://www.embopress.org/doi/full/10.1038/emboj.2012.61

A paper that I was not aware of that @embojournal scientific editor @HVodermaier mentioned in the #telomerase independent immortalisation discussion on the latest #EMBOPodcast episode is the finding from Lackner, Karlseder et al in #EMBOJournal 2012 of a very interesting ALT system in #Celegans

🎧 to the episode
https://www.embo.org/podcasts/alternative-immortality/

Read the paper (#OpenAccess)
https://www.embopress.org/doi/full/10.1038/emboj.2012.61

A paper that I was not aware that @embojournal scientific editor @HVodermaier brought in the #telomerase independent immortalisation discussion on the latest #EMBOPodcast episode is the finding from Lackner, Karlseder et al in #EMBOJournal 2012 of a very interesting ALT system in #Celegans

🎧 to the episode
https://www.embo.org/podcasts/alternative-immortality/

Read the paper (#OpenAccess)
https://www.embopress.org/doi/full/10.1038/emboj.2012.61

The latest episode of the EMBO Podcast #EMBOPodcast is out 🎧

I spoke with Roger Reddel & Tracy Bryan at Sydney's Children's Medical Research Institute about their classic 1995 @embojournal paper describing #telomerase independent #telomere lengthening in immortalised cell lines. It challenged conventional wisdom at the time - and it was Bryan's PhD project, quite a way to get started in science.

@miguelgf provided some perspective on the work and its enduring significance and editor @HVodermaier explained #EMBOJournal's interest in studies with striking findings that may not provide a full mechanistic description.

You can listen to "ALTernative immortality" at the link below or on your preferred podcast app. Special thanks to Igor Jukic & audio producer extraordinaire @marcoantonio

https://www.embo.org/podcasts/alternative-immortality/

#Telomeres #Telomerase #tumorigenesis #Preprint from Charles Kinzig, Titia de Lange et al

"Here, we aimed to determine whether telomerase can add telomeric DNA to DSBs in human cells and how this process is regulated. The data indicate that telomerase can create a functional neotelomere at a Cas9-induced DSB that bears the TS sequence at one 3′ end. The frequency of neotelomere formation is increased upon overexpression of telomerase, suggesting that the low level of telomerase in most human cells minimizes these deleterious events. In addition, neotelomere formation is inhibited by ATR signaling at resected DSBs. We discuss these findings in the context of genome instability during tumorigenesis, where neotelomere formation by telomerase might end BFB cycles as originally proposed by McClintock."

https://www.biorxiv.org/content/10.1101/2022.10.31.514589v1