🔥 📣 This is probably bad timing, with all the political chaos in Israel, but we're looking for a PhD student to continue a grad student's project on mRNA transfer through tunneling nanotubes.
Start date ~Jan 2024.
We are looking for someone interested in deciphering the molecular mechanism of mRNA transfer, by performing a CRISPR/siRNA/drug screen, and using molecular biology & biochemistry tools.
(more next post >>)

#mRNA #RNA #mRNALocalization #TunnelingNanotubes

https://www.weizmann.ac.il/molgen/Gerst/research-activities/cell-cell-transfer-mrna-and-its-role-mammalian-cell-physiology

Several years ago, we published our work (in collaboration with
Arjun Raj) on the transfer of mRNA between mammalian cells via cellular connections called #tunnelingnanotubes.

https://www.pnas.org/doi/full/10.1073/pnas.1706365114

The problem with this work was that it was a very partial picture. Because we used single molecule FISH, we had information on transfer of only a small number of mRNAs.
Relatively early, I asked what is the extent of mRNA transfer? Can we find the whole transferome"?
>>

🔥🔥 🔥 📣 Our paper in
@eLife is finally published!

"Global analysis of contact-dependent human-to-mouse intercellular mRNA and lncRNA transfer in cell culture"

https://elifesciences.org/articles/83584

#mRNA #TunnelingNanotubes

I'd like to thank the two students Sandipan Dasgupta and Daniella Dayagi who did most of the work, for Markus Landthaler
and @ewyler for doing the sequencing, Tsvyia Olender for most of the bioinformatics and Rob & Jeff for promoting and supporting this complicated project.
>>

🙋‍♂️Tomorrow!
I'm giving a seminar at university of Michigan RNA innovation seminar series.
12 PM (EST) 7pm (israel) MONDAY - FEB 13th *Virtual Only*

"Why Did the RNA Cross the Nanotube?"

#mRNA #TunnelingNanotubes

with zoom: https://umich.zoom.us/webinar/register/WN_bp1ZbK8QRgq-GeRu3Pw7hA#/registration

@Datta_Lab That is very interesting!
But if you do not detect viral RNA/protein, how can you tell if the virus does NOT also infect OSN?
In this animal study https://www.cell.com/cell/fulltext/S0092-8674(22)00135-0 they found ~6% OSN infected.
In addition, this study https://www.science.org/doi/10.1126/sciadv.abo0171 showed that the virus CAN infect ACE2-negative cells (e.g. neurons like OSN) through #TunnelingNanotubes
Did anyone look at biopsies from the acute phase?

So, after lurking here for a couple of days, I realized that hashtags are important here. So here are a few of my interests:
#RNA
#mRNA
#mRNALocalization
#ribosome #P_bodies #StressGranules
#Transcription #Translation #mRNADegradation
#CellBiology #MolecularBiology
#TunnelingNanotubes
#EndoplasmicReticulum #mitochondria #Peroxisome
#Microscopy #fluorescent #FluorescentProteins
#PeerReview #StaffScientist
#Reproducibility
#LabLife