Does #ScienceMastodon have a #NoStupidQuestions type hashtag yet? Where can I ask my seemingly obvious, borderline embarassing questions?
Like why can't I reuse running/transfer buffer in #WesternBlot or #Electrophoresis for #PCR gels?
Why can't I just reuse agarose gels for PCR after imaging them by putting them back in electrophoresis and letting all the #DNA run off?

If you're my PI and you me asking this, no you didn't.

Any suggestions on a commercially available Rabbit anti-FLAG antibody that works well for WB analysis? I had bad luck with this one from #CellSignalingTechnology: DYKDDDDK Tag (D6W5B) Rabbit mAb (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #14793

#protein #immunoblotting #westernblot #biochemistry #cellbiology

Does anyone know a good GPX4 #antibody for #WesternBlot and #Immunofluorescence reactive to humans and mice?