Kostas Kormas · @kkormas
118 followers · 174 posts · Server mstdn.science
Jon Moller · @guofu
268 followers · 3955 posts · Server mastodon.sdf.org
EPPIcenter at UCSF · @EPPIcenter_UCSF
175 followers · 33 posts · Server genomic.social

Exploring SSAfrica plasmodium genetic diversity using microsatellite variants & SNPs in resistance genes

@malariagenomics
@epiverse

nature.com/articles/s41598-022

#malaria #genomic #microsatellite #genotyping #amplicon

Last updated 2 years ago

EPPIcenter at UCSF · @EPPIcenter_UCSF
83 followers · 16 posts · Server genomic.social
Jim Downie · @cenococcum
94 followers · 15 posts · Server mstdn.science

Hi all, curious if anyone has run into this kind of issue before? We're quantifying our reactions for using PicoGreen prior to normalisation, but we're sometimes getting high concentrations despite no band being visible on a gel. For ITS it’s only at 61 ng/ul that there’s a 50/50 chance of seeing a band. Any idea what might be going on?

#amplicon #pcr #Metabarcoding #microbiology #molecularBiology #labwork

Last updated 2 years ago