Let’s talk about genome engineering and temperature.

Almost all CRISPR modalities are based on enzymes. And enzymes care about temperature. So we probably know a lot about CRISPR at different temps. Or not???

A 🧵

#CRISPR #geneediting #CFDbaseedit

Creating loss-of-function mutations with cytidine base editors (CBE). Our new preprint is online. Work of the incredible Roman Doll (part! of his master thesis).

#CRISPR #geneediting #baseediting #Drosophila #CFDbaseedit

We set up a very efficient system to install premature stop codons or disrupt splice sites in Drosophila and made some interesting discoveries along the way. Will unpack these in separate threads in the coming days. For now here is the manuscript:

https://www.biorxiv.org/content/10.1101/2022.12.13.520203v1