Researchers uncover novel physiological functions of #CRISPR-Cas guard #RNA.

https://phys.org/news/2023-09-uncover-physiological-functions-crispr-cas-rna.html

Meharry Medical College student KeAndreya Morrison highlighted a publication with contributions from Andreas Plückthun's group that appeared in Gene Therapy and describes a novel all-in-one shock and kill method for HIV-1 therapy that shows a promising way to eliminate infected cells in a specific T-cell and HIV-1 manner.

https://www.tumblr.com/sbgridconsortium/727088151128096768/all-in-one-shock-and-kill-therapies-could-be-the?source=share

#sbgrid
#science
#biomedicalresearch
#crispr
#therapeutic

Listen up, #CRISPR Fan(zor)atics! ✂️🧬 In a new #MolecularCell spotlight, Costas Patinios, a postdoc in #HIRI’s Beisel lab, recaps Feng Zhang's recent remarkable discovery describing eukaryotic #RNA-guided DNA endonucleases, called Fanzors. Read more here: https://authors.elsevier.com/a/1hjJC_OylrlCTl

We are hiring project scientists, Postdocs and research associates in multiple labs. Please reach out if you are looking for exciting #phage #microbiology #synbio #CRISPR #AMR #evolution #ecology Systems biology & infectious disease biology work. 🙏

Cracking the functional domains of the RADX gene during replication. 🧬

Jessica Chevallier & Pierre Caron together highlight work from the #CortezLab which is now available online, ahead of print #JMolBiol.

Read the preLight! 👉https://prelights.biologists.com/highlights/crispr-dependent-base-editing-screens-identify-separation-of-function-mutants-of-radx-with-altered-rad51-regulatory-activity/

#CRISPR #GenomeStability #Replication #DSBs

heise+ | Gentechnik: Konkurrenz für CRISPR durch die Rekombinasen

Die Designer-Rekombinasen schneiden das Erbgut nicht nur äußerst präzise, sondern reparieren es auch gleich. Im Herbst soll eine erste klinische Studie starten.

https://www.heise.de/hintergrund/Gentechnik-Konkurrenz-fuer-CRISPR-durch-die-Rekombinasen-9237022.html?wt_mc=sm.red.ho.mastodon.mastodon.md_beitraege.md_beitraege

#CRISPR #DNA #Gentechnik #Leben #Medizin #news

#CRISPR arrays are a compact way for nature to encode #gRNAs, but their ability to acquire new spacers keeps them longer than necessary for CRISPR technologies. #HIRI's Beisel lab now shows that CRISPR-Cas9 arrays can be shortened without compromising: https://doi.org/10.1080/15476286.2023.2247247

See-through Hummingbird Bobtail Squids - with the chromophore pigments CRISPR'd out - are so transparent that they are readily tractable for in vivo Ca2+ imaging. Plus, they look adorable!

Creation of an albino squid line by CRISPR-Cas9 and its application for in vivo functional imaging of neural activity
Ahuja et al., Current Biology 2023
https://doi.org/10.1016/j.cub.2023.05.066

Nice writeup by NPR
https://www.npr.org/sections/health-shots/2023/08/28/1196073961/how-scientists-engineered-a-see-through-squid-with-its-brain-in-plain-view

#neuroscience #cephalopods #neurophysiology #crispr

Online now: A CRISPR Screen of HIV Dependency Factors Reveals That CCNT1 Is Non-Essential in T Cells but Required for HIV-1 Reactivation from Latency https://doi.org/10.3390/v15091863. We screened a CRISPR guide library of host genes necessary for HIV replication to identify host factors that are also necessary for HIV to emerge from latency. The top hit, CCNT1, aka CyclinT1, is absolutely necessary for HIV reactivation over a wide range of activation pathways. #virology #HIV #science #CRISPR

We've got some more disease-resistant rice!

UC Davis and many international scientists have used CRISPR to create a new cultivar that is broadly resistant to many diseases.

The gene Resistance To Blast 1 (RBL1), when knocked out, improved resistance, but lowered yields 20 fold. So a mutated allele was made instead to keep the benefits and lose the downsides.

#Rice #Agriculture #CRISPR #GeneEditing #GMO #Biology #Biotech #Biotechnology #Scicomm

https://www.nature.com/articles/s41586-023-06205-2

Gene Editing Could Exterminate the HIV Virus:
For the first time, the Nobel Prize winning CRISPR gene editing technology has successfully inactivated an HIV-like virus from primate cells. #geneediting #crispr #hiv #aids #monkeys #ebt
https://www.instagram.com/p/CwcLTpDrGPD/

双重#CRISPR-Cas3修复了由杜氏肌营养不良症(Duchenne muscular dystrophy)患者来源的干细胞中的dystrophin功能。这项研究有希望为开发治疗杜氏肌营养不良症的新方法提供理论基础。详见@stemcellreports的报道。@CiRA_KU_J 的 Akitsu Hotta是该项研究的参与者。https://ift.tt/XdSOUhT 🔗原始的网页链接是：https://ift.tt/r3laNvo

More developments in making gene drives using CRISPR.

In order to combat Drosophila suzukii fruit destroying flies, NC State researchers have created a "homing" gene drive targeting the fly doublesex gene that, if inhibited, will result in sterility of the next generation.

Since the gene is so conserved, it could also be used as a target with other harmful insects.

#CRISPR #GeneDrive #Flies #Sterility #Biology #Science #Biotech #Biotechnology #Scicomm

https://www.pnas.org/doi/10.1073/pnas.2301525120

CRISPR Beyond the Lab: trasformare le industrie e rimodellare il futuro
L'impatto della tecnologia CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) va ...
#biocarburanti #Biotecnologiaindustriale #ClusteredRegularlyInterspacedShortPalindromicRepeats #CRISPR #ingegneriagenetica

https://bloginnovazione.it/tecnologia-crispr/40047/

This #CRISPR result - an HIV-like virus was eradicated from non-human primates - is encouraging. I also have a strong, selfish interest in seeing it used as a treatment for genetic conditions like the #TERT #pulmonaryfibrosis mutation that runs in my family. https://www.nature.com/articles/s41434-023-00410-4

Scienziati dell'Università della California e ricercatori australiani hanno creato batteri capaci di "vedere" e rilevare il DNA tumorale negli organismi viventi. Questa metodologia, denominata CATCH, sfrutta la tecnologia CRISPR per esaminare sequenze di DNA a livello genomico, confrontandole con sequenze cancerogene note. Immaginate un futuro in cui la diagnosi precoce è la norma, non l'eccezione. Questa può essere la chiave.

#Diagnosi #CRISPR #Batteri #Sensori #cancro

https://www.futuroprossimo.it/2023/08/dal-laboratorio-alla-vita-ecco-i-batteri-che-vedono-il-dna-tumorale/

1 Postdoc, 1 Postdoc/Lab manager & 2 PhD students in Immuno-epigenetics

IMol PAS

See the full job description on jobRxiv: https://jobrxiv.org/job/imol-pas-27778-1-postdoc-1-postdoc-lab-manager-2-phd-students-in-immuno-epigenetics/?feed_id=55238

#ScienceJobs #hiring #research #epigenetics #epigenomics #immunology #crispr #degron
Warsaw #Poland #Bioinformatician #Man...
https://jobrxiv.org/job/imol-pas-27778-1-postdoc-1-postdoc-lab-manager-2-phd-students-in-immuno-epigenetics/?feed_id=55238

CRISPR Beyond the Lab: trasformare le industrie e rimodellare il futuro
L'impatto della tecnologia CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) va ...
#biocarburanti #Biotecnologiaindustriale #ClusteredRegularlyInterspacedShortPalindromicRepeats #CRISPR #ingegneriagenetica

https://bloginnovazione.it/tecnologia-crispr/40047/

heise+ | Gentechnik: Erste Erfolge mit #CRISPR – und die Bedenken | MIT Technology Review https://www.heise.de/hintergrund/Heilen-mit-der-Schere-Das-neue-CRISPR-9198261.html #heiseplus #dna

@jeffcliff @donnayoungdc @BowsacNoodle @RogerFalcon @white_male

#CRISPR 🍟