Regenerative Research Fdn/NSCI · @NeuralStemCells
8 followers · 70 posts · Server mstdn.science
Today, Dr. Rosa Mino shared her beautiful work on clathrin-mediated endocytosis, a key process that regulates availability of cell surface molecules in the central nervous system, cancer & other cell types. Thank you, Dr. Mino!
#CRISPRi #tirfmicroscopy
@Nous_MRE
@HustonTillotson
Vivek Mutalik · @vivek_mutalik
645 followers · 730 posts · Server fediscience.org
Vivek Mutalik · @vivek_mutalik
629 followers · 629 posts · Server fediscience.orgInducible #CRISPRi-based operon silencing and selective in trans gene #complementation in #Borrelia burgdorferi | bioRxiv
#genetictools
https://www.biorxiv.org/content/10.1101/2022.12.05.519228v1
#genetictools #borrelia #complementation #crispri
Vivek Mutalik · @vivek_mutalik
627 followers · 620 posts · Server fediscience.org#High-throughput retrieval of target sequences from complex clone libraries using #CRISPRi | Nature #Biotechnology
Vivek Mutalik · @vivek_mutalik
605 followers · 560 posts · Server fediscience.org#CRISPRi-based #programmable logic inverter cascade for #antibiotic-free #selection and maintenance of multiple #plasmids | Nucleic Acids Research | Oxford Academic
#plasmids #selection #antibiotic #programmable #crispri
· @irinavoineagu
10 followers · 1 posts · Server genomic.socialExcited that our work on #CRISPRi #screening of enhancer elements got funded by @NHMRC Ideas.
Fantastic work by talented
@NicoleFGreen @gavinjsutton
implementing this technology in primary cells. Fun times ahead!
Vivek Mutalik · @vivek_mutalik
468 followers · 355 posts · Server fediscience.org#CRISPRi-based programmable logic inverter cascade for antibiotic-free selection and maintenance of multiple #plasmids
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkac1104/6895192
#synbio #logicgates #circuits
#circuits #LogicGates #synbio #plasmids #crispri
Vivek Mutalik · @vivek_mutalik
367 followers · 167 posts · Server fediscience.org
#High-throughput retrieval of target sequences from complex clone #libraries using #CRISPRi
#highthroughput #Metagenomics #functional #crispr #crispri #libraries #high
Jan-Willem Veening · @jwveening
47 followers · 7 posts · Server mastodon.socialNew paper now published in #mSystems by Pascal Hols' lab with some help from us (Johann Mignolet) where 3 cool genome-wide #CRISPRi screens are used to study competence in #Streptococcus #salivarius. It finds that #competence increases upon cell wall impairment with a role for #StkP
https://journals.asm.org/doi/10.1128/msystems.00735-22
#crispri #msystems #streptococcus #salivarius #competence #stkp