Cool in summer? Cucumber slices!🥒

🔬 Our researchers slice cells & mini-brains with precision on a 200µm scale using Aquilos2 microscope. They investigate diseases by imaging tiny molecules with electrons, combining with SOLIST technique for faster prep: weeks to days!

Thanks to @3P1L @KalebicNereo Flaminia Kaluthantrige Don Jasmine Nguyen @gaiaperone

🧐 Learn more about SOLIST: https://www.biorxiv.org/content/10.1101/2023.05.11.540146v1

#lifesciences #humantechnopole #science #sciencemastodon #cryoem #cryoet #biology

Our Sam Lacey, Helen Foster and Gaia Pigino from the Structural #Biology Research Centre conquer the cover of #Nature Structural & Molecular Biology!

Using a combination of #cryoET and structure prediction approaches, they reveal the molecular structure of IFT-A and IFT-B trains, providing insights into anterograde transport of cargo into the cilia tip.

Read more about the research 👉 https://humantechnopole.it/en/publications/the-molecular-structure-of-ift-a-and-ift-b-in-anterograde-intraflagellar-transport-trains/

#sciencemastodon #science #humantechnopole @Nature@mstdn.social @nature@sciencemastodon.com @Nature@press.coop

RT @Intein
We’re happy to share our #CryoET work of native #muscle in the #mavacamten relaxed state with thin #actin & thick #myosin, #titin, #MyBPC filament.

Thanks to #teamtomo @mpimoph, esp @DvdGently @maxplanckpress in collab with Gautel lab
@KingsCollegeLon

https://www.biorxiv.org/content/10.1101/2023.04.11.536387v1

Fantastic #cryoem #cryoet Rotavirus study from @proteincapsid in the Mark Boyce and David Stuart labs @OxfordStrubi. Truly amazing to see the potential of in situ methods to address the dynamic nature of proteins and reveal previously unseen conformations. https://www.cell.com/cell-host-microbe/fulltext/S1931-3128(23)00108-7

Our non-canonical nucleosome study has been updated. tl;dr - lots of VPP #cryoET; canonical nucleosomes account for < 10% of the total expected in situ. Work of Zhi Yang Tan, Shujun Cai, Alex Noble, and Jon Chen.
https://www.biorxiv.org/content/10.1101/2021.04.04.438362v2

How does #vaccinia #virus assemble in infected cells? #CryoET shows how spherical immature virions mature into infectious brick-shaped mature virus, with 2 consecutive protein lattices driving assembly & maturation @MiguelHdez_3 @DrMichaelWay #PLOSBiology https://plos.io/3EN2KLm

How does #vaccinia #virus assemble in infected cells? #CryoET shows how spherical immature virions mature into infectious brick-shaped mature virus, with 2 consecutive protein lattices driving assembly & maturation @MiguelHdez_3 @DrMichaelWay #PLOSBiology https://plos.io/3EN2KLm

How does #vaccinia #virus assemble in infected cells? #CryoET shows how spherical immature virions mature into infectious brick-shaped mature virus, with 2 consecutive protein lattices driving assembly & maturation @MiguelHdez_3 @DrMichaelWay #PLOSBiology https://plos.io/3EN2KLm

RT @SchurLab
We have two ERC-funded Postdoc positions available to work on our project ActinID.
More info here:
https://schurlab.ista.ac.at/open-positions/
Feel free to reach out for more info.

Please help spreading the word and retweet!
#structuralbiology #cellbiology #cryoEM #cryoET #teamtomo

Fantastic to see our SPTLabtech colleagues at #BPS2023. Why not drop in on booth 821 and to hear more about the latest developments in grid technology - and of course our new building! #cryoem #structuralbiology #cryoet

Just wow! Very impressive work by @svenklumpe on further developing and employing #cryoET. Thanks for the great talk and for @ramundo_silvia for inviting Sven that we could all get inspired!
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RT @svenklumpe
It’s been very inspiring to visit GMI @viennabiocenter and present my research. Thank you for the invite @ramundo_silvia!!! Simply amazing to discuss science with @plaschka_lab @haselbachlab @PamelaVetrano @juliusbrennecke @PauliG…
https://twitter.com/svenklumpe/status/1626613412158484480

Ready for Microscopy & Microanalysis 2023 in Minneapolis, MN July 23-27?

Consider submitting your abstract to B02 - 3D Structures: From Macromolecular Assemblies to Whole Cells (3DEM FIG). Additional speakers will be chosen from abstracts. Call for Submissions closes 11:59 PM PST on Thursday, February 16, 2023.

Apply today: https://www.microscopy.org/MandM/2023/

#CryoEM #cryoET #microscopy

#CryoET news:

Quantification of gallium cryo-FIB milling damage in biological lamella

"We find that the mechanism of FIB-milling damage is distinct from radiation damage during cryo-EM imaging. By accounting for both electron scattering and FIB-milling damage, we find that FIB-milling damage will negate the potential improvements from lamella thinning beyond 90 nm."
http://biorxiv.org/cgi/content/short/2023.02.01.526705v1?rss=1

Ah yes the Powerhouse of the cell *strokes beard*

#CryoET #StructuralBiology @strucbio Cryo-electron tomography of C. elegans mitochondria reveals how the ATP synthase dimer interface shapes crista membranes http://biorxiv.org/cgi/content/short/2023.02.02.526626v1?rss=1

RT @rdrighetto
I reprocessed the T. kivui 70S ribosomes (EMPIAR-11058) with #RELION-4 to get a ~7 Å map in situ 🤩

This map is now deposited @EMDB_EMPIAR as EMD-16451:
https://www.ebi.ac.uk/emdb/EMD-16451

#OpenSoftwareAcceleratesScience
#TeamTomo #CryoET

RT @PlevkaLab
📣 We're looking for a #postdoc!
Are you interested in #viruses and #structuralbiology? Apply and join our team of enthusiastic researchers to work on cutting-edge #cryoEM #cryoET projects! 🔬
Please RT :)
@instructhub #postdocposition #ERC https://twitter.com/CEITEC_Brno/status/1617436466053009409

Looking to do more #cryoET or more time-resolved? Fill out this survey to tell us how you'd like to see #cryoem #sampleprep change with times. The first 48 respondents will receive a high-quality pair of tweezers as a thank you

#cryoEM #cryoET #jobs #career opportunity: the structural biology department at Genentech is hiring a computational scientist to join its #cryoEM team. https://careers.gene.com/us/en/job/GENEUS202212143696EXTERNALENUS/Computational-Scientist-3-Cryo-EM-Structural-Biology

Fascinating read -- Asgard archaea imaged by #cryoET:

News & Views
https://www.nature.com/articles/d41586-022-04450-5

Article
https://www.nature.com/articles/s41586-022-05550-y

RT @zauggj@twitter.com

Are you interested in #cryoET data mining and linking to (single-cell) genomics? Reach out, we are hiring. Check out our work in this space: "Convolutional networks for supervised mining of molecular patterns within cellular context" https://www.biorxiv.org/content/10.1101/2022.04.12.488077v1

🐦🔗: https://twitter.com/zauggj/status/1603022787404242953