Hi #teamMassSpec looking for some tips on how to search DIA SILAC data using DiaNN , does anyone have any suggestions? #DiaNN #SILAC

A question for all the DiaNN experts here: I run DiaNN through FragPipe 19.1 and tried to see if there is a big deal in the "Unrelated runs" option in FragPipe for DiaNN. I totally understand the ID data differing, but not why the Total.Quantity if different for both run modi, though the MS1 and MS2 Quantities are identical?!? (in red the difference) #FragPipe #DiaNN