That iSeq (unfortunate acronym collision) uses cre/lox and a split URA3 marker to recombine or not genomic barcodes. So we have known arrays of single-barcoded yeasties that you can cross to an array of unknown, recombine and amplicon seq to determine the location - aka REDIseq (Smith et al 2017 or so?)

I'm trying to think how you could use this to do tetrad phenotyping in pools.... surely a paper has figured that out in the last 5 years 😅

#dumbBarcodeTricks #halfbaked