Polisarioarekin zuzenean negoziatu beharko luke EBk hurrengo arrantza ituna

https://www.berria.eus/paperekoa/1920/020/002/2023-07-18/polisarioarekin-zuzenean-negoziatu-beharko-luke-ebk-hurrengo-arrantza-ituna.htm

#MendebaldekoSahara #Sahara #Arrantza #Maroko #EB #EuroparBatasuna #Europa

Chips in the rain, Saturday edition #eb

🇵🇱 #Marketing nie musi być nudny, przewidywalny, czy irytujący. Choć zewsząd bombardują nas komunikaty marketingowe, od czasu do czasu zdarza się, że ktoś wyjdzie poza ramy i zrobi to odrobinę inaczej.

Fajnym przykładem kampanii #EmployerBranding, która ujrzała światło dzienne już dawno temu, jest dla mnie to, co we współpracy z jedną z agencji, zrobiło #hebe

Przyjemne, kolorowe plakaty, zabawa słowem nadająca podwójnego znaczenia komunikatom i przekaz oddający to, co marka faktycznie chciała przekazać - nie boli, nie irytuje, a wręcz przyciąga uwagę.

#kampaniereklamowe #reklama #EB

Pixel City - Tiny Rebel - 4.4% ABV. B+ and a bit, murky, fruity and a little sharp with a lingering fruity, bitter finish #ale #beer #bier

3.3/5. Turning into a Cask Saturday at @tinyrebelnpt Great staff and a loyalty app as well. Fine Ales and Fine WiFi too #eb #beersofmastodon @beersofmastodon

Graeme Souness swimming the English Channel to raise £1.1m to stop the pain of EB | DEBRA UK
#EB
#GraemeSouness
#EnglishChannelSwim
#IslaGrist
#EpidermolysisBullosa
#ButterflySkin
#NHS
#DEBRA
#Charity
#CharitySwim
https://www.debra.org.uk/news/graeme-souness-swimming-the-english-channel-to-raise-11m-to-stop-the-pain-of-eb

https://donate.giveasyoulive.com/fundraising/debraswim

Mistmode - Polly’s Brew Co - 4.9% ABV. B++, lime and passion fruit sour. Gently sour, highly fruity that really hits the spot #ale #beer #bier

3.5/5. Brewed in North Wales, consumed in South Wales 🏴󠁧󠁢󠁷󠁬󠁳󠁿 Tidy brew plus at @weirddadbrewery #eb #beersofmastodon @beersofmastodon

Maverick - Vale Of Glamorgan Brewery Brewery - 4.5% ABV. B+ and a bit, hoppy, malty, fruity, bitter and refreshing Pint. Like it #ale #beer #bier

3.3/5. Great to see Barry’s finest back in the Cask game. This is turning out to be a very good Saturday #Newport #eb #beersofmastodon @beersofmastodon

Midas Touch - Wye Valley - 4.8% ABV. B+ and a bit, malty, fruity and hoppy. With a fruity bitter lingering finish. Nice #ale #beer #bier

3.3/5. A sunny Saturday at the @oenwignewport and there are new Cask Ales on and things are good with the WiFi and the World #eb #beersofmastodon @beersofmastodon

@SakuraRin 文心一言水印去除：
yiyan.baidu.com###eb-watermark
把它丢到 adblockers 里就好了

Ce qu’il faut savoir sur la nouvelle distribution EB corbos Linux.

Elektrobit et Canonical annoncent EB corbos Linux, une nouvelle distribution Linux spécialisée pour le marché automobile.

Elle sera basée sur Ubuntu, avec une collaboration directe de son éditeur.#EB corbos Linux https://lsdm.live/modules/news/article.php?storyid=3734

Ekstrabladet kommer til at få rigtigt meget fedt indhold i det her format.
Kim Christiansen: Ikke blevet tilbudt “Det sidste ord”
Katja K: Ikke blevet tilbudt “Det sidste ord”
Din mor: Ikke blevet tilbudt “Det sidste ord”
#ekstrabladet #EB #detsidsteord

Laag water op het Wad, live: https://bekijkhet.nu/cams-wd.html#terschelling

#terschelling #eb #waddenzee

Skinful - Beartown - 4.2% ABV. B+, says earthy and spicy and that’s just what I’m getting. Getting a touch of fruit in the finish #ale #beer #bier

3/5. Another hard January Frost in South East Wales however I’m on an Emergency Beano #eb 🍻 #beersofmastodon @beersofmastodon

Skeletal #muscle is a type of tissue that makes up a large part of the human body. It is made up of many different cells that are able to contract and move. Skeletal muscle has the ability to #repair itself when it is damaged due to #aging, exercise, or diseases like #MuscularDystrophy. A small group of cells called #SatelliteCell s help with the repair process. Scientists have been trying to create models to study how #Skeletalmuscle develops and regenerates. Recently, they have been using human pluripotent #StemCell to create 3D models of skeletal muscle tissue. However, these models have not been able to recreate the full process of muscle regeneration. In this research paper, the authors introduce a new method of using human pluripotent stem cells to create 3D models of skeletal muscle tissue that can retain the ability to repair itself.

Over the past decades, scientists have used #animalmodel to study #muscleregeneration, which is regulated by #stemcell s. These animal models have been very helpful in understanding the mechanisms of muscle #regeneration, but they don't always accurately reflect the same range of diseases that humans experience. Therefore, researchers have suggested creating reliable in vitro models using human muscle cells. ( #hPSC s) could be used to create 3D human skeletal muscle #organoid s ( #hSkMO s) that contain sustainable #stemcell and distinct myofibers with the same proteins and structure as adult muscles. Previous approaches to skeletal muscle differentiation have been developed using 2D #culture systems, but these lack the natural environment and #StemCell niche that are necessary to model adult #myogenesis and muscle #regeneration.

#Stemcell s ( #SC s) can be used to repair damaged muscle tissue. They explain that SCs can be activated in response to muscle injuries and that other #cell types can contribute to the process of #myogenesis. The author then goes on to explain that #cytokine s, such as IL-4, can influence the #InflammatorySystem and promote SCs differentiation, which helps with muscle regeneration. While #organoid s generated from #hPSC s have potential, they do not fully replicate the in vivo native microenvironment. To address this, treat the #hSkMO s with extrinsic #cytokine s to promote #muscle #regeneration . #hSkMO s might then be used to study aspects of human muscle #biology and to identify novel #therapeutic candidates for muscle-wasting disorders.

To create a 3D structure of muscle tissue. They used #WNT activator and #BMP inhibitors at the beginning of the differentiation process to induce paraxial #mesodermal #cell s. They then added #FGF2 to the Matrigel to promote the 3D structure. #HGF and IGF1 were added later to accelerate the #myogenic specification and further #myofiber differentiation. They optimized the timing of the Matrigel embedding to day seven. After this, they observed #neuralcell s and withdrew FGF2 to focus on muscle tissue development. They then prolonged the HGF and IGF1 treatment to propagate #myogenic #progenitor s. They found that 62% of the #tissue was #skeletalmuscle tissue and that it contained PAX7+ #myogenic #stem / #progenitor cells, MYOD+ activated/committed #myoblast s, and MYOG+ #myocyte s. They also found that 31% of PAX7+/Ki67− and 29% of MYOD−/PAX7+ non-dividing quiescent SCs were present in the mature #hSkMO s. This indicates that the #hSkMO s were able to effectively recreate #embryo nic #myogenesis and have regenerative potential. Future studies using #singlecell #RNA sequencing may be necessary to further characterize the different types of cells in #hSkMO s.

The stepwise process to generate human skeletal muscle organoid s (hSkMOs) from human pluripotent stem cells (hPSCs)

The process begins with dissociating #hPSC s into #singlecell s and allowing them to form #embryoid bodies ( #EB s) in low-attachment V-shaped 96-well plates. Then, paraxial #mesodermal differentiation is promoted with #WNT activation, BMP inhibition, and FGF2 signaling. The expression of pluripotency markers OCT4 and NANOG decreases, and the expression of #mesoderm markers Brachyury, T-Box transcription factor 6 (TBX6), and mesogenin 1 (MSGN1) increases. To further characterize paraxial #mesoderm al differentiation, TBX6 is #immunostain ed. After paraxial #mesodermal induction, the #organoid s are embedded with growth factor-reduced Matrigel and transferred to a six-well plate on an orbital shaker. Growth factors are then added to the #myogenic specification media, and #hSkMO s are cultured until the day of analysis. The orbital shaker improves the viability, survival, and differentiation of hSkMOs by increasing the penetration rate of oxygen and nutrients into the core area of hSkMOs. The #hSkMOs gradually grow to more than 1.5 mm in diameter by day 60, appearing round-shaped, uniformly sized, and having relatively homogenous morphology. PAX3 and PAX7 are #myogenic progenitor markers, and their expression is verified by qRT-PCR and #cryo sections. The #myogenic cells appear as clusters, and approximately 9% of PAX7+ cells are double-positive for Ki67 at day 30, demonstrating that proliferating cells are #myogenic #progenitor s in hSkMOs. This indicates that the in vitro #hSkMO #culturesystem is able to recapitulate the features of embryonic skeletal #muscle development.

The different types of #SkeletalMuscle stem/progenitor cells that are involved in myogenesis, the process of muscle formation.

The researchers used qRT-PCR analysis and #immunohistochemistry to identify and characterize the different types of cells. They found that PAX3 and PAX7 (SC markers) were the major population during the early stage of #myogenesis, and that MYOD (proliferating and activated SC marker) and MYOG (differentiated myocyte marker) increased over time. They also observed that MYOD−/PAX7+, MYOD+/PAX7+, and MYOD+/Ki67+ cells accounted for 29%, 6%, and 8% of the putative quiescent, activated, and proliferating #SC s, respectively. MYOD+/PAX7− cells constituted 39% of differentiating myoblasts, and MYOG−/PAX7+ cells constituted 23% of putative quiescent SCs. MYOG+/PAX7− cells accounted for 30% of differentiated #myocyte s, and 8% and 6% of the MYOG+ cells in #hSkMO s co-expressed PAX7 and Ki67, respectively. This data shows that the researchers were able to identify and characterize different types of skeletal muscle stem/progenitor cells during #myogenesis.

The text is discussing the results of a research study that used hSkMOs (human skeletal muscle #organoid s) to study the development of skeletal muscle #tissue. The study found that the #hSkMO s grew exponentially in size within two months, and the growth rate then steadily decreased. The researchers then used scanning electron microscopy (SEM) imaging and confocal microscopy to examine the cytoarchitecture of the hSkMOs. They found that the hSkMOs contained a large population of terminally differentiated #myogenic cells and a small population of preserved myogenic stem/progenitor cells. They also found that the hSkMOs contained a substantial proportion of TITIN+ muscle cells and MAP2-positive #neuron s. To further characterize the presence of sustainable stem cells within the mature hSkMOs, they quantified the amount of dormant stem cells by #confocal #microscopy imaging. The results showed that approximately 56%, 31%, and 5% of PAX7+/Ki67- putative dormant stem cells existed throughout the differentiation of hSkMOs at days 30, 70, and 130, respectively. This indicates that the hSkMOs contained mature skeletal muscle properties and had the potential for #regeneration .

The researchers wanted to see if the #hSkMO s (human #skeletal muscle #organoid s) had the ability to regenerate #muscle #tissue after damage. To test this, they treated the hSkMOs with a cardiotoxin (CTX) which is known to induce muscle inflammation and damage. They then observed a decrease in PAX7+ and MYOD+ cells in the hSkMOs. To further test the #regenerative potential of the #hSkMO s, they added interleukin-4 (IL-4) to the medium to promote #muscleregeneration. After 14 days, they observed a significant increase in MYOG+ myocytes in the CTX-injured hSkMOs with the treatment of IL-4 compared to the CTX-injured hSkMOs without the treatment. This suggests that the hSkMOs have the potential to regenerate muscle tissue after damage.

#explainpaper

Generation of Skeletal Muscle Organoids from Human Pluripotent Stem Cells to Model Myogenesis and Muscle Regeneration

Authors :

Min-Kyoung Shin , Jin Seok Bang , Jeoung Eun Lee , Hoang-Dai Tran , Genehong Park , Dong Ryul Lee and Junghyun Jo

别再随意亲孩子！7岁女孩高烧一周被确诊亲吻病 https://news.ifeng.com/c/8LHofZwmLGS #自限性 #高烧 #eb #女孩 #病毒 #孩子 #郑州 #脏器

King Sano - Tiny Rebel - 7.2% ABV. B++, big, amplified Mosaic IPA. Fruity, peppery with a lingering bitter finish #ale #beer #bier

3.5/5. Missing the Big Fridge to the side of the bar @tinyrebelnpt Solid cannage and strong to boot #eb #beersofmastodon

Rift - Electric Bear - 3.8% ABV. B+ and a bit, fruity, peppery with a gentle, lingering fruity bitter finish #ale #beer #bier

3.3/5. At the other end of @tinyrebelnpt and the @threeuk signal is still only 1 bar. Drinking Bath’s finest #eb #beersofmastodon

Bale Ale - Glamorgan Brewing - 4.5% ABV. B+ and a bit, malty base with notes of citrus and lemon coming through. Sharp citrus bitter finish #ale #beer #bier

3.3/5. Now we are going to the World Cup 🏴󠁧󠁢󠁷󠁬󠁳󠁿 And a good 9p a pint cheaper than in Hereford and Gloucester #eb #beersofmastodon

Oprør i fagbevægelse imod at afskaffe forbeholdet. Hvorfor skal ⁦@FH_dk2019⁩ mv. overhovedet have en holdning til 1.juni..? Hvad har sagen med fagbevægelsen at gøre? #eb #dkpol #stemnej

🐦🔗: https://nitter.eu/MrMesserschmidt/status/1529713911439794176

Super Hero - Tudor Brewery - 4.5% ABV. B+ and a bit, big malty base, complex slightly peppery body with a slightly bitter peppery finish #ale #beer #bier

3.2/5. From just up the road from the @penwignewport In Wales, drinking Welsh in the pleasant May sun. #eb #beersofmastodon