Happy #EMmonday! This week we are highlighting research from Riley Cooney, Eszter Vladar and colleagues. They find that DKK3 and WNT4 work together to enable a switch from canonical to non-canonical Wnt signalling during multiciliogenesis.

https://journals.biologists.com/jcs/article/136/16/jcs260807/326574/A-WNT4-and-DKK3-driven-canonical-to-noncanonical

This #EMmonday we are looking at axoneme organisation with Tomohiro Kubo, Toshiyuki Oda and colleagues. They find that that the glutamate-rich C-terminal tails of α- and β-tubulin play essential, and distinct, roles in ciliary motility and assembly.
Highlight: https://journals.biologists.com/jcs/article/136/16/e136_e1601/325932/Roles-of-ciliary-tubulin-PTMs-in-Chlamydomonas

It’s #EMmonday and this week we’re looking at RPE cells counterstained using the OTO method to highlight lipid-rich structures. Kevin Zhang, Joshua Dunaief and colleagues discover that oxidative stress from iron loading induces lysosome dysfunction and ceramide accumulation in RPE cells.
📖 @DMM_Journal: https://journals.biologists.com/dmm/article/16/7/dmm050066/324837/Oxidative-stress-induces-lysosomal-membrane

It’s #EMmonday and we’re looking at ER-phagosome contact sites with Mayis Kaba, Nicolas Demaurex and colleagues. They find that Nir2 and Nir3 are recruited to these contact sites and sustain phosphoinositide signalling in phagocytosis.
📖 @J_Cell_Sci: https://journals.biologists.com/jcs/article/136/14/jcs260902/324832/The-lipid-transfer-proteins-Nir2-and-Nir3-sustain

Happy #EMmonday! We’re looking at the @DMM_Journal paper from Tejia Zhang, Randall Peterson and colleagues examining different approaches for sphingolipidosis treatment in their new zebrafish model of combined saposin deficiency.
https://journals.biologists.com/dmm/article/16/7/dmm049995/319488/A-zebrafish-model-of-combined-saposin-deficiency

#microscopy #zebrafish #electronmicroscopy #diseasemodel

This #EMmonday we’re highlighting the 3D architecture of the zebrafish germline cyst by SBF-SEM from Vineet Kumar & Yaniv Elkouby. Check out their materials and methods for the manual segmentation protocol using TrakEM2.
https://journals.biologists.com/dev/article/150/13/dev201349/321182/Tools-to-analyze-the-organization-and-formation-of

#microscopy #CellBiology #volumeEM

This #EMmonday we’re looking at fly myofibrils from Nicanor González Morales, Frieder Schöck and colleagues. They find that sarcomeres are disrupted in the absence of oxoglutarate dehydrogenase complex due to small Z-discs.
📖 @J_Cell_Sci: https://journals.biologists.com/jcs/article/136/13/jcs260717/320616/The-oxoglutarate-dehydrogenase-complex-is-involved

This #EMmonday we are focussing on SBF-SEM with a case study from Federica Mangione. Federica and colleagues used SBF-SEM to dissect the cellular assembly of tactile organ of Drosophila, identifying a new cell type, the F-Cell.

https://focalplane.biologists.com/2023/05/19/inputs-and-outputs-of-vem-in-a-sensory-system/

#volumeEM #drosophila #devbio

For more from the volume EM community: https://focalplane.biologists.com/category/volume-em/

This #EMmonday we are looking at these beautiful L. mexicana axoneme cross-sections from Chandra Ramakrishnan, Cécile Fort, Linda Kohl, Richard Wheeler, Robert Sinden and colleagues. They examined the role of Rsp9 in axoneme assembly.
#OpenAccess

https://journals.biologists.com/jcs/article/136/11/jcs260655/316601/Radial-spoke-protein-9-is-necessary-for-axoneme

This #EMmonday we are focussed on SEM, highlighting the cochleas of Gipc3 knockout mice. Paroma Chatterjee, Peter Barr-Gillespie (Oregon Health & Science University, Portland) find that GIPC3 orchestrates forces, form and function during hair cell morphogenesis.

📖@J_Cell_Sci: https://journals.biologists.com/jcs/article/136/10/jcs261100/310737/GIPC3-couples-to-MYO6-and-PDZ-domain-proteins-and

It’s #EMmonday and we’re looking at mitochondria with Siavash Khosravi, Max Harner and colleagues. They discover a new mitochondria contact site formed by the inner membrane protein Cqd1. Overexpression of Cqd1 result in altered mitochondria architecture.

https://journals.biologists.com/jcs/article/136/10/jcs260578/310504/The-UbiB-family-member-Cqd1-forms-a-novel-membrane

It’s #EMmonday and for the second week in a row we are appreciating beautiful mitochondria! Siavash Khosravi, Max Harner and colleagues find that Cqd1 forms novel contact sites in mitochondria and is important for maintaining mitochondrial homeostasis.

@J_Cell_Sci: https://journals.biologists.com/jcs/article/doi/10.1242/jcs.260578/307108/The-UbiB-family-member-Cqd1-forms-a-novel-membrane

#cellbiology #cell #mitochondria #electronmicroscopy

This #EMmonday we are looking Drosophila eyes by scanning electron microscopy (SEM). In their study, Andreia Oliveira, Carla Lopes, et al. identify Stg/Cdc25 phosphatase as a suppressor of abnormal Tau phosphorylation and its associated toxicity.

@DMM_Journal: https://journals.biologists.com/dmm/article/16/1/dmm049693/286728/String-Cdc25-phosphatase-is-a-suppressor-of-Tau

Good things come in 3s. It’s #EMmonday and we are looking at tripartite synapse formation via a trans-interaction between astrocytic Necl2 and axonal Necl3 with Osamu Nozawa, Muneaki Miyata, Kiyohito Mizutani, Yoshimi Takai and colleagues.

📖@Dev_journal: https://journals.biologists.com/dev/article/150/4/dev200931/287111/Necl2-3-mediated-mechanism-for-tripartite-synapse

Spiralling out of control on #EMmonday! Check out the invaginations of the Toxoplasma parasitophorous vacuole (PV) in a host CHMP4B DN mutant.
Julia Romano, Isabelle Coppens and colleagues discover that Toxoplasma hijacks the host ESCRT-III machinery for uptake of nutrients into the PV.
@J_Cell_Sci: https://journals.biologists.com/jcs/article/136/4/jcs260159/287078/Toxoplasma-gondii-scavenges-mammalian-host

You can find out more about the research in Julia’s ‘First person’ interview: https://journals.biologists.com/jcs/article/136/4/jcs261049/287077/First-person-Julia-Romano

This #EMmonday we are looking at mitochondrial morphogenesis during sperm flagellum formation from Meng-Yan Chen, Ya Zheng, Yu-Feng Wang and colleagues. They discover that cytochrome c1-like is required for mitochondrial morphogenesis during spermatogenesis in Drosophila.

@J_Exp_Biol: https://journals.biologists.com/jeb/article/226/3/jeb245277/286906/Cytochrome-c1-like-is-required-for-mitochondrial

It’s #EMMonday and we’re looking at #mitochondria morphology.

Gabriella Robertson, Vivian Gama and colleagues examine mitochondria behaviour in fibroblasts derived from patients with EMPF1 due to mutations in DRP1.

@J_Cell_Sci: https://doi.org/10.1242/jcs.260370

#microscopy #TEM #cellbio

This #EMmonday we’re looking at microvesicle release from inner segments of #photoreceptors. #TEM image shows microvesicle next to endocytic, clathrin-coated pit.

📖 @DMM_Journal: https://doi.org/10.1242/dmm.049871

#Microvesicle #Retina #Vision #Science #FocalPlane #Biology #Imaging