Anyone else doing plasma proteomics with short nano gradients on an Ultimate3000 (<1h) and FAIMS/Exploris480 combined? I thought we had the golden rule of only 1 CV with shorter gradients lengths, but seems to be not true in terms of peptide-centric working people. #Ntermini #FAIMS #TeamMassSpec

@Chrashwood @phillyoung @michaelsteidel @MattWFoster

I wonder if people observe this on #ionmobility instruments (that separate in charge dimension too)?

Maybe an easy way to answer the question is with #FAIMS on vs off? Does the net unfrag change in these #DIA runs under each condition?