The use of multiple #fluorophores in a sample and the choice of #FilterSets can be challenging. Depending on the desired combination of fluorophores, filters may or may not prevent bleed through. There are also factors other than the optics to consider. Learn more about using fluorophores: https://ilphotonics.com/understanding-and-using-fluorophores/

☑️ If you want to image more subcellular targets in parallel, this on-demand webinar “Live-cell fluorescence lifetime multiplexing using organic fluorophores” is right for you.
💹 You'll discover how you can expand your multiplexing capabilities using FLIM. Speaker is Dr. Michelle Frei.
https://fcld.ly/0kjk03c

#confocal #fluorescence #fluorophores #ScienceTwitter #ScienceMastodon

"Real-Time, Multicolor Fluorescence Imaging of Tumors in Tissues": https://doi.org/10.56537/new-ground.2022.22694 - Thorough presentation of Venu G. Bandi et al., 2022 https://doi.org/10.1038/s41592-022-01394-6 in @naturemethods #journal #imaging #fluorophores

I came across this #microscopy paper. https://www.nature.com/articles/s41587-022-01519-4
"By blue-shifting the #STED beam and separating #fluorophores by on/off switching, individual fluorophores bound to a #DNA strand are localized with σ = 4.7 Å, corresponding to a fraction of the fluorophore size, with only 2,000 detected #photons ."
This image showing resolution below the size of the fluorophore leaves me speechless.
The paper features images of #DNAorigami, #NuclearPores, synaptic #Vesicles, #ECM (#laminin) and #vilmentin