This week’s Research Spotlight is on the Wang et al. (2023) publication, “Multimodal mapping of cell types and projections in the central nucleus of the amygdala.” #HCRimaging helped Wang et al. determine spatial, morphological, and connectivity properties of molecularly defined central nucleus amygdala neurons. Read their work in more detail here: doi.org/khzs

#neurons #amygdala #withHCR

This week’s Research Spotlight is on the Nobori et al. (2023) publication, “Multiplexed single-cell 3D spatial gene expression analysis in plant tissue using PHYTOMap.” In this study, Nobori et al. utilized #HCRimaging to verify the expression of GFP in plant tissues. This validation process served to confirm the accuracy and reliability of their new method, PHYTOMap. Learn about PHYTOMap in more detail here: doi.org/gsb7vn

#research #spotlight #plant #tissue

This week’s User Spotlight is on Jeffrey Chen, a postdoctoral fellow in the Nelson Lab at the Indiana University School of Medicine! #HCRimaging enabled Jeffrey and the Nelson Lab to determine the spatial and qualitative expression of TMPRSS3 in mouse cochlea. Read their work in more detail here: doi.org/kffx.

#user #spotlight #gene #expression #mouse

This week’s User Spotlight is on Jeffrey Chen, a postdoctoral fellow in the Nelson Lab at the Indiana University School of Medicine! #HCRimaging enabled Jeffrey and the Nelson Lab to determine the spatial and qualitative expression of TMPRSS3 in mouse cochlea. Read their work in more detail here: doi.org/kffx.

#user #spotlight #gene #expression #mouse

This week’s Research Spotlight is on the Thomas et al. (2023) publication, “Voltage-gated sodium channel activity mediates sea urchin larval skeletal patterning through spatial regulation of Wnt5 expression.” Through #HCRimaging, Thomas et al. was able to establish Wnt5 as a vascular endothelial growth factor (VEGF)-independent skeletal patterning cue. Read their work in more detail here: doi.org/kdnp

#HCRimaging #research #gene #expression

This week’s Research Spotlight is on the Thomas et al. (2023) publication, “Voltage-gated sodium channel activity mediates sea urchin larval skeletal patterning through spatial regulation of Wnt5 expression.” Through #HCRimaging, Thomas et al. was able to establish Wnt5 as a vascular endothelial growth factor (VEGF)-independent skeletal patterning cue. Read their work in more detail here: doi.org/kdnp

#HCRimaging #research #gene #expression

This week’s User Spotlight is on Jun Takatoh, a research scientist in the Wang Lab at MIT! Through HCR RNA-FISH, Jun has been able to gain insight into the connectivity and molecular profiles of the neural circuits that regulate orofacial behaviors. “[HCR RNA-FISH] significantly streamlines the process of dissecting neural circuits.” Check out his stunning image #withHCR here:

#FluorescenceFriday #mouse #HCRimaging

This week’s #ResearchSpotlight is on the Shi et al. (2023) publication, “Single-cell transcriptomic profiling of the zebrafish inner ear reveals molecularly distinct hair cell and supporting cell subtypes.” #HCRimaging helped Shi et al. validate zebrafish as a relevant model for understanding inner ear-specific hair cell function and regeneration due to the similarities of inner ear cell type composition between fish and mammals. Read their work in more detail here: doi.org/gr39p2

#zebrafish

This week’s User Spotlight is on Sadie Bergeron, an associate professor at the WVU Departments of Biology and Neuroscience! Sadie appreciates how #HCRimaging enables researchers to visualize mRNA localization within neurons. “It’s been really great to compare with colorimetric in situ hybridization results.” Check out her latest publication #withHCR here: doi.org/j996

#neurons #mRNA

This week’s User Spotlight is on Janani Ramachandran, PhD, a postdoctoral researcher in the Vokes Lab at the The University of Texas at Austin! HCR RNA-FISH helped Janani understand the role of Hedgehog signaling in the development of the mouse larynx. #withHCR’s multiplexing features, she was able to visualize the expression domains of several genes along the developing foregut and larynx. Check out her work in more detail here: doi.org/j8mt

#user #spotlight #mouse #HCRimaging

This week’s Research Spotlight is on the Truong et al. (2023) publication, “PRDM1 DNA-binding zinc finger domain is required for normal limb development and is disrupted in split hand/foot malformation.” Truong et al. conducted whole-mount HCR RNA-FISH to validate their RNA-seq data and determine the effect of prdm1a loss on gene expression. Check out Brittany Truong’s paper here: doi.org/j7k8

#research #spotlight #HCRimaging

This week’s User Spotlight is on Julia Peloggia de Castro, a PhD candidate in the Piotrowski Lab at the Stowers Institute for Medical Research! Julia studies developmental plasticity using the zebrafish lateral line and ionocytes as models. #withHCR, she is able to map gene co-expression with single cell resolution even in crowded tissues like the lateral line. Check out Julia’s stunning image here:

#user #spotlight #FluorescenceFriday #zebrafish #HCRimaging

This week’s Research Spotlight is on the Zhu et al. (2023) publication, “Protein expression/secretion boost by a novel unique 21-mer cis-regulatory motif (Exin21) via mRNA stabilization.” Zhu et al. performed single-molecule #HCRimaging to evaluate the role of Exin21/Qα in regulating the dynamic changes of targeted mRNA in HEK293T cells. Read their work in more detail here: doi.org/grtf97

#research #spotlight #protein #mRNA

This week’s User Spotlight is on Joshua Raji, a postdoctoral student in the Potter Lab at The Johns Hopkins University School of Medicine! Joshua appreciates that whenever he visualizes his images on the confocal microscope #withHCR, “the signals are bright with less background, and it always works like magic!” Check out his stunning HCR RNA-FISH images of the mosquito antenna below:

#user #spotlight #HCRimaging #FluorescenceFriday #mosquito #antenna

This week’s Research Spotlight is on the Lopéz et al. (2023) publication, “Molecular characterization of nervous system organization in the hemichordate Saccoglossus kowalevskii!” #HCRimaging enabled Lopéz et al. to characterize the expression of multiple neural markers, which revealed S. kowalevskii’s strong regional specialization of its nervous system. Read their work in more detail here: doi.org/j4mz

#research #spotlight #HCRimaging #acorn #worm

This week’s User Spotlight is on Eric Horstick, an assistant professor at the West Virginia University Department of Biology! #withHCR, Eric was able to test for gene expression and neural activity changes in vivo that would have otherwise been challenging with other methods. View his beautiful HCR RNA-FISH images of larval zebrafish below, and check out his lab page for more info here: horsticklab.org.

#user #spotlight #HCRimaging #FluorescenceFriday #zebrafish

This week’s User Spotlight is on Petra Popovics, an assistant professor at the Eastern Virginia Medical School! While she was a former assistant scientist in the Ricke Lab at the University of Wisconsin-Madison, HCR™ RNA-FISH helped Petra identify different immune cell types in the mouse prostate and localize Spp1 expression in luminal microphages. Check out her latest publication #withHCR here: doi.org/j265.

#user #spotlight #HCRimaging #FluorescenceFriday

This week’s User Spotlight is on Cambrian Liu, a research assistant professor and group leader of the Cambrian Liu Lab at The University of Chicago Pritzker School of Medicine! Cambrian appreciates the simplicity of HCR RNA-FISH. “The protocol and multiplex labeling were much easier than ACDBio’s.” Check out his stunning images #withHCR of mouse FFPE sections in the colon, small intestines, and anus here:

#user #spotlight #HCRimaging #FluorescenceFriday

As we celebrate International Women's Day this year, let's recognize the invaluable contributions that #WomeninScience have made across all industries. Thank you to all the phenomenal women at MI who have each played a crucial role in making #HCRimaging a reality! #IWD2023

This week’s User Spotlight is on Madeline Ryan, a research technician in the Tsai Lab at the Washington University School of Medicine in St. Louis! The HCR Platform’s ability to visualize the expression of multiple genes at the same time has helped Madeline to map out cell-type specific expression of various adhesion molecules in the zebrafish spinal cord. Check out her stunning images #withHCR here:

#FluorescenceFriday #user #spotlight #HCRimaging