A new paper from @wu_xuebing 's lab just published @Nature
"Noncoding translation mitigation"

https://www.nature.com/articles/s41586-023-05946-4

Here are my thoughts:
It is a very interesting paper, and important to anyone working on #translation, particularly from #ncRNAs, but not only.
In brief, they screened short sequences originating from #lncRNA or from non coding regions ( #UTR #intron) (and #mRNA CDS as control) which they fused to GFP at the C-term and looked at GFP expression level.
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I stopped being a research scientist over six years ago but I still receive Google Scholar alerts when my publications are cited.

This week a new paper about #intron length prominently cited some work I led on. This is the new paper:

‘The position of the longest intron is related to biological functions in some human genes’

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9875286/

Have any antisense oligonucleotide #ASO experts made it onto mastodon?

I'm trying to find evidence on how much of a role #intron secondary structure remediation plays in splice-switching ASO design. A presentation by an ASO researcher from a biotech stated that the #spliceosome recognizes intron #secstruct, particularly the intron 5' structure, but haven't found published evidence or hypothesis.

#pre-mRNA #splicing

@edwardlau #intron do any of the transcipts contain tRNA or mRNA introns ?

https://www.nature.com/scitable/topicpage/translation-dna-to-mrna-to-protein-393/