A #CRISPR-Cas9-tagging approach to induce #chromosome-specific segmental #aneuploidies: targeted assembly of ectopic #kinetochores at endogenous repeats - a way to recapitulate and study specific disease-associated #missegregation events in human cells

@McClellandLab
https://www.embopress.org/doi/10.15252/embj.2022111587

#Parasitology paperwatch: recent review from Midori Ishii & Bungo Akiyoshi on #centromeres and #kinetochores. Principally describes the distinction between monocentric and holocentric centromeres, the role of the histone H3 variant CENP-A (when present) and their taxonomic distribution. It then touches on #kinetoplastid kinetochores and the riddle of what defines their centromeres at the end. 😀 👍 👏

https://www.sciencedirect.com/science/article/pii/S0955067422000011