MCDuncanLab · @MCDuncanLab
245 followers · 1566 posts · Server mstdn.social

ARGH!!
Lab's been having a weird problem with western blots. They look like people are touching their blots with dirty hands.

I'm doing a couple of experiments and having the same problem and I *know* I am not touching blots with dirty hands.

Guess what our G*dmned gloves are leaving fluorescence on the blots.

From left to right, rubbed with my washed bare fingers, or two glove brands washed or unwashed with handsoap.

#Science #lablife

Last updated 1 year ago

MCDuncanLab · @MCDuncanLab
243 followers · 1523 posts · Server mstdn.social

With the start of the academic year, I want to repost my expert learning article. Soon, graduate students will start their first lab rotations. A lucky few will walk in, and things will work seemingly magically on day one. For most, this marks the start of a sometimes arduous journey of learning to be an expert experimentalist. As a PI, have you ever wondered how to make this journey less arduous? 1/🧵

molbiolcell.org/doi/10.1091/mb

#phd #gradschool #graduateschool #lablife #academiclife #mentoring

Last updated 1 year ago

Dave nλ=2dsinθ :protein: · @xtaldave
1572 followers · 1610 posts · Server xtaldave.net

Nothing like a -80°C freezer failure to get the blood pumping.

#lablife

Last updated 1 year ago

ErrantScience · @errantscience
1892 followers · 345 posts · Server mas.to
MCDuncanLab · @MCDuncanLab
225 followers · 1370 posts · Server mstdn.social

Assembling a strains table for a manuscript. >100 yeast strains. Wow!! I owe my lab a happy hour.

#Science #pilife #lablife #tetraddissection

Last updated 1 year ago

Dave nλ=2dsinθ :protein: · @xtaldave
1466 followers · 1478 posts · Server xtaldave.net

It's that time of year when I have to remind folks to clean up after themselves on the FPLCs.

#lablife

Last updated 1 year ago

Maciek Majdecki · @MajdeckiMaciek
724 followers · 54 posts · Server mas.to
Stephen Royle · @steveroyle
892 followers · 104 posts · Server biologists.social

This is an interesting piece on “Why every lab needs a handbook”. By this they mean a set of established principles that the lab collectively adheres to. To help set expectations and sense check behaviour etc.

elifesciences.org/articles/888

#academicchatter #lablife #lifeofpi

Last updated 1 year ago

MCDuncanLab · @MCDuncanLab
190 followers · 1123 posts · Server mstdn.social

I learned that you should never use a binocular microscope with only one eye.

I'm working with a scientist with a lot of years under their belt but maybe not the best prior training. They seem unable/unwilling to use both eyes. For our compound scope, I know this is a problem because they need depth perception for certain manipulations. Is it also a problem for a compound microscope?

Are there tips I can share to help them?

thx!

#benchwork #pilife #imaging #microscopy #lablife

Last updated 1 year ago

MCDuncanLab · @MCDuncanLab
170 followers · 1052 posts · Server mstdn.social
DaveGPT :protein: · @xtaldave
1423 followers · 1129 posts · Server xtaldave.net

Today is apparently one of those days when every communal reagent I go to use will have run out and I will need to make a big batch from scratch because some bastard couldn't be bothered to make a new batch when *THEY* used the last of it.

#lablife #Science

Last updated 1 year ago

MCDuncanLab · @MCDuncanLab
151 followers · 973 posts · Server mstdn.social
MCDuncanLab · @MCDuncanLab
162 followers · 1022 posts · Server mstdn.social
MCDuncanLab · @MCDuncanLab
149 followers · 958 posts · Server mstdn.social

You periodic reminder that digital notebooks are awesome!!

I'm working on a manuscript. I know a former lab member worked on the project when we thought it was going in direction X, but now we realize its direction Y. It only took 40 minutes for me to find and review each piece of data.

This saves me work in not having to repeat experiments I've forgotten about, and allows me to accurately attribute contributions to people who are no-longer in the lab.

#Science #academia #pilife #lablife

Last updated 1 year ago

MCDuncanLab · @MCDuncanLab
162 followers · 1022 posts · Server mstdn.social

Your periodic reminder that digital notebooks are awesome!!

I'm working on a manuscript. I know a former lab member worked on the project when we thought it was going in direction X, but now we realize its direction Y. It only took 40 minutes for me to find and review each piece of data.

This saves me work in not having to repeat experiments I've forgotten about, and allows me to accurately attribute contributions to people who are no-longer in the lab.

#Science #academia #pilife #lablife

Last updated 1 year ago

Laborjournal · @laborjournal
534 followers · 321 posts · Server mstdn.science

Während unsere TA fleißig pipettiert, entdeckt eine Kollegin in einer Packung Chamber Slides etwas Seltsames. Es ist klein, süß und schwarz …

— "Der Indikatorkäfer", neue Folge unserer "Erlebnisse einer TA": 👉 laborjournal.de/editorials/276

#labtechnician #lablife

Last updated 1 year ago

ErrantScience · @errantscience
1466 followers · 263 posts · Server mas.to
Lynn R-E · @lynnrankinesquer
8 followers · 31 posts · Server sfba.social

Find something you love as much as my dog Dash loves pillows. He literally drags them into position to get them just right.

#DogLife #livingeasy #lablife #doggylove

Last updated 1 year ago

LZVolk · @lzvolk
104 followers · 539 posts · Server mas.to

One of the many joys of getting to the lab early before others. 🙄.

#lablife

Last updated 2 years ago

Elzi Volk · @lzvolk
90 followers · 535 posts · Server fediscience.org

@c0nc0rdance You got it!
Consider the lab manager’s job for $42,000/yr to be a mommy to grad students & post-docs, teacher, troubleshooter, negotiator, editor, supply & biotech coordinator, budget hawk, mediator for PIs who forget how to do bench work, and occupational hazard monitor. All so that PIs can sit and write all day at desks in plush offices, chat on the phone, & drink cocktails and joke at conventions. 😏

#lablife

Last updated 2 years ago