@PhiloNeuroScie Nice find! #Tissueclearing and #lightsheet #microscopy work on human brain (sections) too :)

Human brain samples are normally quite hard to properly image because they are dense with lipofuscins, super autofluorescent and not very permeable to antibodies. That they managed to pull this off using the more-or-less standard iDISCO protocol (even with "just" a 4 mm thick slab and high antibody concentrations) is quite impressive.

edits: corrected thickness + typos

@ottiswimmers I haven't tried this yet, but there is a recent biorxiv paper on a DIY #lightsheet #microscope that uses a FLIR Grasshopper 3. They have controller code on github too, designed to run on an Nvidia Jetson Nano, I assume that would be running Linux.

Paper:
https://www.biorxiv.org/content/10.1101/2023.05.31.543173v2.full

Controller code on github:
https://github.com/tomerlab/pLSM-Control

Check out our lab's #newpaper in @cellpress Cell:
We found that the skull is special - its marrow is a special immunologic niche. Skull #Immune #cells can move to the meninges via skull-meninges connections, which we found both in mice and humans with #tissueclearing and #lightsheet #microscopy.
We also bring #proteomics and #PETimaging to the mix!

Paper: https://doi.org/10.1016/j.cell.2023.07.009

Explainer thread:
https://bird.makeup/users/erturklab/statuses/1689293936185991169

Preprint alert! Our lab developed targetable #DNA origami #nanoparticles, which we then image throughout the entire (transparent) mouse body using #lightsheet #microscopy.

Our DNA origami were able to target immune cells, as well as a subset of cancer metastases in the lung.

Preprint:
https://www.biorxiv.org/content/10.1101/2023.07.24.550304v1.full.pdf

Explainer thread:
https://bird.makeup/users/erturklab/statuses/1686331791022448640

#preprint #tissueclearing

Happy to introduce our latest gizmo from the lab: pLSM !
https://www.biorxiv.org/content/10.1101/2023.05.31.543173v1

A big shoutout to our brilliant grad student, Yannan Chen, who led this work.

In short, pLSM is a plug-and-play, cost-effective and easy-to-scale light sheet microscopy platform. More than 20 times cheaper, with imaging quality comparable to high-end systems.

https://github.com/tomerlab/pLSM-Control

#LSFM #preprint #biorxiv #lightsheet #dopamine

@naelnadif #FluorescenceFriday #lightsheet #microscopy #imageanalysis

@elab @rami @maels @Fairy @neuronflow #neuroscience #preprint #lightsheet

RT @PatrickLemaire_
Oyez, Oyez, we are seeking to expand our team by recruiting 3 new colleagues, #PhDstudents and #postdoc. If you love #LiveImaging, #lightsheet, #scRNAseq, #smFISH, #QuantitativeBiology, #ascidians, #embryos and, please read on (and RT) 👇

#sciencejobs @SFbd @ISDB @TLM @SMBdevBio

RT @voigtvision
This image reminds me of an early (2017-ish) @MesoSpim #lightsheet #Microscopy dataset I took from a Clarity-cleared TPH2-tdTomato sample - the first time I saw the flatness of Purkinje cell dendrites with my own eyes https://twitter.com/BrainsRus/status/1642875805570154497

New preprint by @elab : Check it out! We mapped SARS-CoV2 spike protein distribution in 3D throughout the mouse body, and at the human skull/brain interface.

We found spike protein accumulation in the skull-meninges-brain axis, lingering long after infection.

Preprint: https://www.biorxiv.org/content/10.1101/2023.04.04.535604v1.full.pdf

Explainer thread: https://twitter.com/erturklab/status/1643902019088908289

#covid #longcovid #lightsheet #tissueclearing #microscopy

My postdoc work at Isaac Bianco's lab is finally out on @eLife !
https://elifesciences.org/articles/78381

Combining #lightsheet volumetric functional imaging and a spiking network simulation, we were able to show that a simple connectivity motif in the #zebrafish #optictectum explains multiple features in ongoing population dynamics, their impact on trial-to-trial variability, and trends in experience dependent behavior.

It is a neat example of how #networkdynamics can explain why neuronal and behavioural responses vary, or how past activity sets the ever-changing background for new input (that, in turn, will make its own long-lasting splash)

Who doesn't love a bit of morphogenesis and light sheet?🔬

Registration is needed.

#lightSheet #LSFM #SPIM #evoDevo #morphogenesis #drosophila
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RT @luxendo
We are incredibly excited to welcome Dr. Matteo Rauzi as webinar speaker.

Studying #Morphogenetic Waves with Photo Manipulation Coupled to Multi-View Light‑Sheet Microscopy

22-03-2023 — 10AM PDT | 1PM EDT
Sign-Up:
https://buff.ly/41pnMcz

#evoDevo #science #LSFM
https://twitter.com/luxendo/status/1631630005062057985

RT @loicaroyer
We present #Zebrahub: a timecourse atlas of zebrafish embryonic development, combining #scRNAseq time-course data with #lightsheet live imaging. Explore our seq. and imaging datasets interactively at

http://www.biorxiv.org/content/10.1101/2023.03.06.531398v1.article-info

http://zebrahub.org
1/n

I want to live in zebrahub
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RT @loicaroyer
We present #Zebrahub: a timecourse atlas of zebrafish embryonic development, combining #scRNAseq time-course data with #lightsheet live imaging. Explore our seq. and imaging datasets interactively at

http://www.biorxiv.org/content/10.1101/2023.03.06.531398v1.article-info

http://zebrahub.org
1/n
https://twitter.com/loicaroyer/status/1633362751040151553

RT @loicaroyer
We present #Zebrahub: a timecourse atlas of zebrafish embryonic development, combining #scRNAseq time-course data with #lightsheet live imaging. Explore our seq. and imaging datasets interactively at

http://www.biorxiv.org/content/10.1101/2023.03.06.531398v1.article-info

http://zebrahub.org
1/n

New post on FocalPlane

Our preprints list this week focusses on research that uses microscopy tools to answer questions in #cellbio and #devbio, featuring #volumeEM, #lightsheet, #TIRF, #SMLM, #CLEM and #bioimageanalysis!

https://focalplane.biologists.com/2023/02/17/microscopy-preprints-applications-in-cell-and-developmental-biology/

#whatareyoureading

Attended the @zeiss_micro #lattice #lightsheet workshop. Very impressive piece of kit. I knew what I expected from attending the talks in #ACMM27 but. We need one in #adelaide.

One pain a day. One acronym a day. #lightsheet #SPIM #LSFM and yes 160th!
Thanks to a keen follower @MicroEos
here is the 160Th and what an acronym MUSI (microscopy with ultraviolet single-plane illumination)...they missed MUSIC by one Correlative...
https://www.biorxiv.org/content/10.1101/2023.01.02.522477v1.full

One pain a day. One acronym a day. #lightsheet #SPIM #LSFM THE END
And to avoid the withdrawal syndrom I will give a few doses in the coming days...You are welcome to use them in your talks!

ne pain a day. One acronym a day. #lightsheet #SPIM #LSFM THE END
Thanks to @glaevsky
and @LSFMMBL
I started this horrrible 🧵 and I will stop there and slowly upload everything on @github
for people to play with and add more...My last count is a 159...yes 159!