Moritz Negwer · @moritz_negwer
314 followers · 1733 posts · Server mstdn.science

@PhiloNeuroScie Nice find! and work on human brain (sections) too :)

Human brain samples are normally quite hard to properly image because they are dense with lipofuscins, super autofluorescent and not very permeable to antibodies. That they managed to pull this off using the more-or-less standard iDISCO protocol (even with "just" a 4 mm thick slab and high antibody concentrations) is quite impressive.

edits: corrected thickness + typos

#tissueclearing #lightsheet #microscopy

Last updated 1 year ago

Moritz Negwer · @moritz_negwer
265 followers · 1349 posts · Server mstdn.science

@ottiswimmers I haven't tried this yet, but there is a recent biorxiv paper on a DIY that uses a FLIR Grasshopper 3. They have controller code on github too, designed to run on an Nvidia Jetson Nano, I assume that would be running Linux.

Paper:
biorxiv.org/content/10.1101/20

Controller code on github:
github.com/tomerlab/pLSM-Contr

#lightsheet #microscope

Last updated 1 year ago

Moritz Negwer · @moritz_negwer
265 followers · 1350 posts · Server mstdn.science

Check out our lab's in @cellpress Cell:
We found that the skull is special - its marrow is a special immunologic niche. Skull can move to the meninges via skull-meninges connections, which we found both in mice and humans with and .
We also bring and to the mix!

Paper: doi.org/10.1016/j.cell.2023.07

Explainer thread:
bird.makeup/users/erturklab/st

#newpaper #immune #cells #tissueclearing #lightsheet #microscopy #proteomics #petimaging

Last updated 1 year ago

Moritz Negwer · @moritz_negwer
265 followers · 1350 posts · Server mstdn.science

Preprint alert! Our lab developed targetable origami , which we then image throughout the entire (transparent) mouse body using .

Our DNA origami were able to target immune cells, as well as a subset of cancer metastases in the lung.

Preprint:
biorxiv.org/content/10.1101/20

Explainer thread:
bird.makeup/users/erturklab/st

#DNA #nanoparticles #lightsheet #microscopy #preprint #tissueclearing

Last updated 1 year ago

Raju Tomer · @rajutomer
32 followers · 36 posts · Server mstdn.science

Happy to introduce our latest gizmo from the lab: pLSM !
biorxiv.org/content/10.1101/20

A big shoutout to our brilliant grad student, Yannan Chen, who led this work.

In short, pLSM is a plug-and-play, cost-effective and easy-to-scale light sheet microscopy platform. More than 20 times cheaper, with imaging quality comparable to high-end systems.

github.com/tomerlab/pLSM-Contr

#LSFM #preprint #biorxiv #lightsheet #dopamine

Last updated 1 year ago

Moritz Negwer · @moritz_negwer
122 followers · 415 posts · Server mstdn.science
Moritz Negwer · @moritz_negwer
122 followers · 415 posts · Server mstdn.science

RT @PatrickLemaire_
Oyez, Oyez, we are seeking to expand our team by recruiting 3 new colleagues, and . If you love , , , , , , and, please read on (and RT) 👇

@SFbd @ISDB @TLM @SMBdevBio

#phdstudents #postdoc #liveimaging #lightsheet #scrnaseq #smfish #QuantitativeBiology #ascidians #Embryos #ScienceJobs

Last updated 1 year ago

Prof Hugo Spiers · @hugospiers
855 followers · 695 posts · Server mastodon.sdf.org

RT @voigtvision
This image reminds me of an early (2017-ish) @MesoSpim dataset I took from a Clarity-cleared TPH2-tdTomato sample - the first time I saw the flatness of Purkinje cell dendrites with my own eyes twitter.com/BrainsRus/status/1

#lightsheet #microscopy

Last updated 2 years ago

Moritz Negwer · @moritz_negwer
68 followers · 118 posts · Server mstdn.science

New preprint by @elab : Check it out! We mapped SARS-CoV2 spike protein distribution in 3D throughout the mouse body, and at the human skull/brain interface.

We found spike protein accumulation in the skull-meninges-brain axis, lingering long after infection.

Preprint: biorxiv.org/content/10.1101/20

Explainer thread: twitter.com/erturklab/status/1

#covid #LongCovid #lightsheet #tissueclearing #microscopy

Last updated 2 years ago

Asaph Zylbertal · @azylbertal
135 followers · 13 posts · Server neuromatch.social

My postdoc work at Isaac Bianco's lab is finally out on @eLife !
elifesciences.org/articles/783

Combining volumetric functional imaging and a spiking network simulation, we were able to show that a simple connectivity motif in the explains multiple features in ongoing population dynamics, their impact on trial-to-trial variability, and trends in experience dependent behavior.

It is a neat example of how can explain why neuronal and behavioural responses vary, or how past activity sets the ever-changing background for new input (that, in turn, will make its own long-lasting splash)

#lightsheet #zebrafish #optictectum #networkdynamics

Last updated 2 years ago

Elisabeth Kugler · @KuglerElisabeth
182 followers · 102 posts · Server mstdn.science

Who doesn't love a bit of morphogenesis and light sheet?🔬

Registration is needed.


---
RT @luxendo
We are incredibly excited to welcome Dr. Matteo Rauzi as webinar speaker.

Studying Waves with Photo Manipulation Coupled to Multi-View Light‑Sheet Microscopy

22-03-2023 — 10AM PDT | 1PM EDT
Sign-Up:
buff.ly/41pnMcz


twitter.com/luxendo/status/163

#lightsheet #LSFM #spim #evodevo #morphogenesis #drosophila #morphogenetic #Science

Last updated 2 years ago

Kay Schink · @kschink
141 followers · 208 posts · Server mstdn.science

RT @loicaroyer
We present : a timecourse atlas of zebrafish embryonic development, combining time-course data with live imaging. Explore our seq. and imaging datasets interactively at

biorxiv.org/content/10.1101/20

zebrahub.org
1/n

#zebrahub #scrnaseq #lightsheet

Last updated 2 years ago

Natasha O'Brown · @Natashaobrown
197 followers · 88 posts · Server mstdn.science

I want to live in zebrahub
---
RT @loicaroyer
We present : a timecourse atlas of zebrafish embryonic development, combining time-course data with live imaging. Explore our seq. and imaging datasets interactively at

biorxiv.org/content/10.1101/20

zebrahub.org
1/n
twitter.com/loicaroyer/status/

#zebrahub #scrnaseq #lightsheet

Last updated 2 years ago

Edwin Hernandez-Garzon · @elfulbito
37 followers · 54 posts · Server qoto.org

RT @loicaroyer
We present : a timecourse atlas of zebrafish embryonic development, combining time-course data with live imaging. Explore our seq. and imaging datasets interactively at

biorxiv.org/content/10.1101/20

zebrahub.org
1/n

#zebrahub #scrnaseq #lightsheet

Last updated 2 years ago

FocalPlane · @focalplane_jcs
349 followers · 124 posts · Server mstdn.science

Attended the @zeiss_micro workshop. Very impressive piece of kit. I knew what I expected from attending the talks in but. We need one in .

#lattice #lightsheet #acmm27 #adelaide

Last updated 2 years ago

Reynaud Emmanuel · @ReynaudEmmanuel
192 followers · 40 posts · Server fediscience.org

One pain a day. One acronym a day. and yes 160th!
Thanks to a keen follower @MicroEos
here is the 160Th and what an acronym MUSI (microscopy with ultraviolet single-plane illumination)...they missed MUSIC by one Correlative...
biorxiv.org/content/10.1101/20

#LSFM #spim #lightsheet

Last updated 2 years ago

Reynaud Emmanuel · @ReynaudEmmanuel
192 followers · 38 posts · Server fediscience.org

One pain a day. One acronym a day. THE END
And to avoid the withdrawal syndrom I will give a few doses in the coming days...You are welcome to use them in your talks!

#LSFM #spim #lightsheet

Last updated 2 years ago

Reynaud Emmanuel · @ReynaudEmmanuel
192 followers · 35 posts · Server fediscience.org

ne pain a day. One acronym a day. THE END
Thanks to @glaevsky
and @LSFMMBL
I started this horrrible 🧵 and I will stop there and slowly upload everything on @github
for people to play with and add more...My last count is a 159...yes 159!

#LSFM #spim #lightsheet

Last updated 2 years ago