Glycans are sweet,
Nucleic acids—they’re fine.
Proteins are interesting,
But nothing beats #lipidtime!

#ValentinesDay #badpoetry

🚨New manuscript: interorganelle lipid transport is rapid, & it is hard to quantify discrete steps in this network, mediated by lipid transfer proteins working at membrane contact sites between different organelles. We (Dongjun Liang & Lin Luan) harness chemoenzymatic tagging & super-fast #bioorthogonal chemistry to make fluorescent lipids that report on PM-to-ER phospholipid flux mediated by a family of lipid transfer proteins at these contact sites! https://doi.org/10.1101/2023.02.01.526230 #lipidtime #chembio

Two surprising take-homes from this work:
1) influenza fusion rates are uncorrelated with target membrane size (and starting curvature)

2) BMP lipids that are enriched in endosomes do affect the efficiency of influenza fusion at the pore formation stage. Previously these lipids had been identified as being important for other viruses but not influenza. Our new assay helps us uncover this dependence. Endosomal lipids are important!

#lipidtime #influenza #viralentry

Thrilled to share this story in JACS Au! https://pubs.acs.org/doi/10.1021/jacsau.2c00419

Takeaways:
1) analogs of PC lipids w/different choline structures adopt different organelle localizations & are useful for tracing interorganelle lipid transport
2) OCT1, a liver transporter typically used to study PK/drug efflux, can also facilitate uptake of small cationic #ChemicalBiology probes, including #bioorthogonal choline analogs. Available soon @Addgene https://addgene.org/188995/

Congrats, Dylan!
#lipidtime #OpenAccess

RT @nadlerlab@twitter.com

Hey everybody, you can find me now also on @nadlerlab Let's recreate #lipidtime over there!

🐦🔗: https://twitter.com/nadlerlab/status/1593613277270900736

Teams #lipidtime #massspectrometry #bioinformatics #chemoinformatics Welcome to Mastodon! Let‘s connect!

Here is another one 👉 Beyond PI3Ks: targeting phosphoinositide kinases in disease @NatRevDrugDisc

▶️ Lots of useful info on phosphoinositide kinase inhibitors, for once not focussed on PI3Ks #lipidtime

https://www.nature.com/articles/s41573-022-00582-5

I am always super excited by the work of Gerry Hammond, here is his newest review: PI(4,5)P2: signaling the plasma membrane

▶️ BONUS! a super nice timeline of the important milestones in the PI(4,5)P2 field. #lipidtime

https://portlandpress.com/biochemj/article/479/21/2311/232084/PI-4-5-P2-signaling-the-plasma-membrane

I am always super excited by the work of Gerry Hammond, here is his newest review: PI(4,5)P2: signaling the plasma membrane

▶️ BONUS! a super nice timeline of the important milestones in the PI(4,5)P2 field. #lipidtime

https://portlandpress.com/biochemj/article/479/21/2311/232084/PI-4-5-P2-signaling-the-plasma-membrane

Lipid-mediated activation of plasma membrane-localized deubiquitylating enzymes modulate endosomal trafficking @NatureComms #lipidtime #trafficking

▶️ Anionic lipids localize and activate a DUB enzyme at the plant plasma membrane

https://www.nature.com/articles/s41467-022-34637-3

Lipid-mediated activation of plasma membrane-localized deubiquitylating enzymes modulate endosomal trafficking @NatureComms #lipidtime #trafficking

▶️ Anionic lipids localize and activate a DUB enzyme at the plasma membrane

https://www.nature.com/articles/s41467-022-34637-3

Hi everyone! I'm @basktastic on Twitter but am trying out my real name here. My #chembio #cellbio #lipidtime lab at Cornell develops tools for imaging and editing the lipidome, drawing on #bioorthogonal chemistry, #clickchemistry, #chemoproteomics, #optogenetics, #directedevolution, and more. https://baskin.wicmb.cornell.edu

Hi everyone! I'm @basktastic on Twitter but am trying out my real name here. My #chembio #cellbio #lipidtime lab at Cornell develops tools for imaging and editing the lipidome, drawing on #bioorthogonal chemistry, #clickchemistry, #chemoproteomics, #optogenetics, #directedevolution, and more. https://baskin.wicmb.cornell.edu