"Boosting the toolbox for live imaging of translation"

The authors developed a new set of #suntag tools (differnt green fluorescent proteins, different promoter for scFv) - optimizing them and identifying possible artefacts. these are primarily for #Drosophila but I guess could be adapted to other organisms.

They also developed a new system called ALFA_array which they test in fly and mammalian cells.

#mRNA #translation #microscopy #LiveCellImaging #SingleMolecule

https://www.biorxiv.org/content/10.1101/2023.02.25.529998v1

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Ok, wow! I just briefly browsed the pics and movies in this preprint from Scott Coyle lab, and they look amazing and beautiful!
looks like an great system for cell tracking, single cell analysis etc...

"A programmable reaction-diffusion system for spatiotemporal cell signaling circuit design"

https://www.biorxiv.org/content/10.1101/2022.11.15.516470v1

#imaging #LiveCellImaging #fluorescent #FluorescentProteins #Microscopy #SyntheticBiology #PrePrint