Is there N6-methyl-2’adenine DNA incorporation derived from decay of #m6A-RNA in cells?

Hailin Wang and colleagues show that mammalian ADAL deaminase sanitizes 2’-#deoxynucleotide pools to prevent AK1-dependent misincorporation

https://www.embopress.org/doi/10.15252/embj.2023113684

Study reveals pivotal #RNA modification in mouse #embryos.

#development #m6A

https://phys.org/news/2023-04-reveals-pivotal-rna-modification-mouse.html

Scientists develop #AI-based method to predict #RNA modifications.

#m6A

https://phys.org/news/2023-02-scientists-ai-based-method-rna-modifications.html

Nice!
Another "uncharacterized" gene (YGL036W) in yeast is now characterized - as encoding a protein (re-named Vir1) that is part of the mRNA m6A methyltransferase complex required during meiosis.

The deletion mutant is defective in meiosis and has zero m6A, just like ime4 deletion.

"Vir1p, the Yeast Homolog of Virilizer, is Required for mRNA m6A Methylation and Meiosis"

#mRNA #m6A #yeast #meiosis

@biorxivpreprint

https://www.biorxiv.org/content/10.1101/2023.02.07.527493v1

Very interesting paper from Schraga Schwartz (in our department) and his student Anna Uzonyi (who won best talk at the latest Israeli #RNA society meeting for presenting this work).
#m6A is an #RNAmodification regulating #mRNADegradation.

They show that m6A is deposited on all #mRNA molecules by default, but is excluded in the vicinity of splice junctions by EJC.
So m6A or lack of it is a memory of nuclear processing.

@MolecularCell

https://www.cell.com/molecular-cell/fulltext/S1097-2765(22)01205-9

Role of #PCIF1-mediated 5′-cap N6-#methyladeonsine #m6A #mRNA #methylation in #colorectalcancer and anti-#PD1 #immunotherapy

New article by Tariq Rana and colleagues at UCSD

https://www.embopress.org/doi/10.15252/embj.2022111673

RNA-binding protein #Rbm14 condensation together with #m6A-modified maternal mRNAs and #RNA #deadenylase Parn controls their #translational competence and decay for vertebrate #blastula #development
New article by Yue Xiao, Jiehui Chen et al:
https://www.embopress.org/doi/10.15252/embj.2022111364

How does N6-methylA (m6A) #RNAmodification influence lncRNAs? @DrPorman @ChromaTownie &co show that the #lncRNA HOTAIR represses or activates genes, depending on a single #m6A modification, to affect #BreastCancer cell biology @rnabioco #PLOSBiology https://plos.io/3VzO5ZT

How does N6-methylA (m6A) #RNAmodification influence lncRNAs? @DrPorman @ChromaTownie &co show that the #lncRNA HOTAIR represses or activates genes, depending on a single #m6A modification, to affect #BreastCancer cell biology @rnabioco #PLOSBiology https://plos.io/3VzO5ZT

How does N6-methylA (m6A) #RNAmodification influence lncRNAs? @DrPorman @ChromaTownie &co show that the #lncRNA HOTAIR represses or activates genes, depending on a single #m6A modification, to affect #BreastCancer cell biology @rnabioco #PLOSBiology https://plos.io/3VzO5ZT