📣 New on my blog:

Re-opening the discussion on mRNA decay inside P-bodies💥

#mRNA #mRNADegradation #Pbodies #phototoxicity

@biorxivpreprint

https://greenfluorescentblog.wordpress.com/2023/05/04/re-opening-the-discussion-on-mrna-decay-inside-p-bodies/

Wow! 🔥
Super interesting pre-print from Bin Wu lab featuring new technology to study mRNA decay by live imaging (I remember Bin suggested this approach 10 yrs ago!)
bottom line:
kinetics of mRNA decay *inside* P-bodies!
Cool paper to read on the train back home tonight!

this new tech allows rapid decay of *all* mRNAs of a certain transcript within minutes.
So it will be useful for other applications for sure.

#mRNA #mRNADegradation #P_bodies

https://www.biorxiv.org/content/10.1101/2023.04.26.538452v1

Interesting paper, looking at mRNA cap1 & cap2.
They claim cap2 marks "aged" mRNAs, i.e. long lived mRNA, and it has immunological importance.
From Samie Jaffrey's lab
@Nature

"mRNA ageing shapes the Cap2 methylome in mammalian mRNA"

#mRNA #mRNADegradation

https://www.nature.com/articles/s41586-022-05668-z

This just published from Jeff Chao's lab
@MolecularCell

"Single-molecule imaging reveals translation-dependent destabilization of mRNAs"

#mRNA #mRNADegradation #miRNA #translation #imaging #SingleMolecule

https://www.cell.com/molecular-cell/fulltext/S1097-2765(23)00033-3

When the bioRxiv came out (along with 2 others on similar topic), I wrote about it in my blog:

https://greenfluorescentblog.wordpress.com/2021/07/25/imaging-of-mirna-mediated-translational-repression-and-mrna-decay-at-single-molecule-resolution/

There are several additions in the print version, e.g. adding miR-21. but its just 1st impression. Have to read it.

Very interesting paper from Schraga Schwartz (in our department) and his student Anna Uzonyi (who won best talk at the latest Israeli #RNA society meeting for presenting this work).
#m6A is an #RNAmodification regulating #mRNADegradation.

They show that m6A is deposited on all #mRNA molecules by default, but is excluded in the vicinity of splice junctions by EJC.
So m6A or lack of it is a memory of nuclear processing.

@MolecularCell

https://www.cell.com/molecular-cell/fulltext/S1097-2765(22)01205-9

📣 💥 It took almost 12 years, but the final paper from my PhD is finally published @naturecomms
It is titled “RNA-controlled nucleocytoplasmic shuttling of mRNA decay factors regulates mRNA synthesis and a novel mRNA decay pathway” but the somewhat dry title hides a novel nuclear import mechanism that we discovered.
https://www.nature.com/articles/s41467-022-34417-z

A 🧵:

#mRNA #mRNADegradation #GeneExpression #yeast #nucleus #NuclearImport #RiboRegulation

So, after lurking here for a couple of days, I realized that hashtags are important here. So here are a few of my interests:
#RNA
#mRNA
#mRNALocalization
#ribosome #P_bodies #StressGranules
#Transcription #Translation #mRNADegradation
#CellBiology #MolecularBiology
#TunnelingNanotubes
#EndoplasmicReticulum #mitochondria #Peroxisome
#Microscopy #fluorescent #FluorescentProteins
#PeerReview #StaffScientist
#Reproducibility
#LabLife