π£ New on my blog:
Re-opening the discussion on mRNA decay inside P-bodiesπ₯
#phototoxicity #pbodies #mRNADegradation #mRNA
Wow! π₯
Super interesting pre-print from Bin Wu lab featuring new technology to study mRNA decay by live imaging (I remember Bin suggested this approach 10 yrs ago!)
bottom line:
kinetics of mRNA decay *inside* P-bodies!
Cool paper to read on the train back home tonight!
this new tech allows rapid decay of *all* mRNAs of a certain transcript within minutes.
So it will be useful for other applications for sure.
#P_bodies #mRNADegradation #mRNA
Interesting paper, looking at mRNA cap1 & cap2.
They claim cap2 marks "aged" mRNAs, i.e. long lived mRNA, and it has immunological importance.
From Samie Jaffrey's lab
@Nature
"mRNA ageing shapes the Cap2 methylome in mammalian mRNA"
This just published from Jeff Chao's lab
@MolecularCell
"Single-molecule imaging reveals translation-dependent destabilization of mRNAs"
#mRNA #mRNADegradation #miRNA #translation #imaging #SingleMolecule
https://www.cell.com/molecular-cell/fulltext/S1097-2765(23)00033-3
When the bioRxiv came out (along with 2 others on similar topic), I wrote about it in my blog:
There are several additions in the print version, e.g. adding miR-21. but its just 1st impression. Have to read it.
#singlemolecule #imaging #translation #miRNA #mRNADegradation #mRNA
Very interesting paper from Schraga Schwartz (in our department) and his student Anna Uzonyi (who won best talk at the latest Israeli #RNA society meeting for presenting this work).
#m6A is an #RNAmodification regulating #mRNADegradation.
They show that m6A is deposited on all #mRNA molecules by default, but is excluded in the vicinity of splice junctions by EJC.
So m6A or lack of it is a memory of nuclear processing.
https://www.cell.com/molecular-cell/fulltext/S1097-2765(22)01205-9
#mRNA #mRNADegradation #rnamodification #m6a #rna
π£ π₯ It took almost 12 years, but the final paper from my PhD is finally published @naturecomms
It is titled βRNA-controlled nucleocytoplasmic shuttling of mRNA decay factors regulates mRNA synthesis and a novel mRNA decay pathwayβ but the somewhat dry title hides a novel nuclear import mechanism that we discovered.
https://www.nature.com/articles/s41467-022-34417-z
A π§΅:
#mRNA #mRNADegradation #GeneExpression #yeast #nucleus #NuclearImport #RiboRegulation
#riboregulation #nuclearimport #Nucleus #yeast #geneexpression #mRNADegradation #mRNA
So, after lurking here for a couple of days, I realized that hashtags are important here. So here are a few of my interests:
#RNA
#mRNA
#mRNALocalization
#ribosome #P_bodies #StressGranules
#Transcription #Translation #mRNADegradation
#CellBiology #MolecularBiology
#TunnelingNanotubes
#EndoplasmicReticulum #mitochondria #Peroxisome
#Microscopy #fluorescent #FluorescentProteins
#PeerReview #StaffScientist
#Reproducibility
#LabLife
#lablife #reproducibility #StaffScientist #PeerReview #FluorescentProteins #fluorescent #microscopy #Peroxisome #mitochondria #EndoplasmicReticulum #TunnelingNanotubes #MolecularBiology #CellBiology #mRNADegradation #translation #transcription #StressGranules #P_bodies #ribosome #mRNALocalization #mRNA #rna