Wow! πŸ”₯
Super interesting pre-print from Bin Wu lab featuring new technology to study mRNA decay by live imaging (I remember Bin suggested this approach 10 yrs ago!)
bottom line:
kinetics of mRNA decay *inside* P-bodies!
Cool paper to read on the train back home tonight!

this new tech allows rapid decay of *all* mRNAs of a certain transcript within minutes.
So it will be useful for other applications for sure.

biorxiv.org/content/10.1101/20

#P_bodies #mRNADegradation #mRNA

Last updated 2 years ago

Interesting paper, looking at mRNA cap1 & cap2.
They claim cap2 marks "aged" mRNAs, i.e. long lived mRNA, and it has immunological importance.
From Samie Jaffrey's lab
@Nature

"mRNA ageing shapes the Cap2 methylome in mammalian mRNA"

nature.com/articles/s41586-022

#mRNADegradation #mRNA

Last updated 2 years ago

This just published from Jeff Chao's lab
@MolecularCell

"Single-molecule imaging reveals translation-dependent destabilization of mRNAs"

cell.com/molecular-cell/fullte

When the bioRxiv came out (along with 2 others on similar topic), I wrote about it in my blog:

greenfluorescentblog.wordpress

There are several additions in the print version, e.g. adding miR-21. but its just 1st impression. Have to read it.

#singlemolecule #imaging #translation #miRNA #mRNADegradation #mRNA

Last updated 2 years ago

Very interesting paper from Schraga Schwartz (in our department) and his student Anna Uzonyi (who won best talk at the latest Israeli society meeting for presenting this work).
is an regulating .

They show that m6A is deposited on all molecules by default, but is excluded in the vicinity of splice junctions by EJC.
So m6A or lack of it is a memory of nuclear processing.

@MolecularCell

cell.com/molecular-cell/fullte

#mRNA #mRNADegradation #rnamodification #m6a #rna

Last updated 2 years ago

πŸ“£ πŸ’₯ It took almost 12 years, but the final paper from my PhD is finally published @naturecomms
It is titled β€œRNA-controlled nucleocytoplasmic shuttling of mRNA decay factors regulates mRNA synthesis and a novel mRNA decay pathway” but the somewhat dry title hides a novel nuclear import mechanism that we discovered.
nature.com/articles/s41467-022

A 🧡:

#riboregulation #nuclearimport #Nucleus #yeast #geneexpression #mRNADegradation #mRNA

Last updated 2 years ago