In 2011, Schwanhäusser, et al. (Nature, 473(7347) measured #absolute #protein & #mRNA levels for 5000 genes in #mamalian cells and informed us 👇
"Protein & mRNA levels correlates 0.41"
Yet, #Reviewer2 demands:
1. #Proteomics validated by #qPCR
2. #RNAseq based #biomarkers
#absolute #protein #mrna #mamalian #Reviewer2 #proteomics #qPCR #rnaseq #biomarkers