calling this Combplex -- combinatorial multiplexing

validated, then adapted to mass-based approaches (MIBI-TOF)

How does the method work? It learns the compression matrix

2/2

weizmann.ac.il/mcb/Keren/home

#massspec #machinelearning #imaging #multiplex

Last updated 1 year ago

PastelBio · @pastelbio
175 followers · 1927 posts · Server mstdn.science

RT @Matrix_Science: You don't need a fancy new search engine to identify peptides from chimeric spectra. Just process the raw file in Mascot Distiller and search with Mascot Server.

matrixscience.com/blog/identif

#proteomics #massspec #massspectrometry #bioinformatics

Last updated 1 year ago

PastelBio · @pastelbio
169 followers · 1756 posts · Server mstdn.science

RT @: Real-Time Spectral Library Matching for Sample Multiplexed Quantitative Proteomics

#jproteomeres #massspec

Last updated 1 year ago

Johannes Rainer · @jorainer
260 followers · 254 posts · Server fosstodon.org

Added support for spectra comparisons using the spectral entropy similarity score to our @bioconductor package (dev version):

tinyurl.com/y6u9wexa

Thanks Yuanyue Li for the :rstats: package and support! 🥳

#massspec #spectra #msentropy #rstats

Last updated 1 year ago

AutoVectis · @autovectis
35 followers · 35 posts · Server mastodon.org.uk

We have a free (already paid for) space available at this year's Ardgour Symposium! More details here - www.emsg-ardgour.site If you can get yourself to Aberdeen on the 20th August, then you could be enjoying a week of mass spec and related discussions, in the Highlands and with a free bar!

#teammassspec #massspec #lipidomics #metabolomics #proteomics #ionization

Last updated 1 year ago

PastelBio · @pastelbio
164 followers · 1673 posts · Server mstdn.science

RT @: What is the most popular / robust/ reliable tool out there for analyzing cross-linking XL-MS data ?

Asking for a friend.

#massspec #proteomics #tools #resources

Last updated 1 year ago

Sarah Hancock · @sarahehancock
148 followers · 3 posts · Server mstdn.science

Just in case anyone in is curious, the likely culprit of my 248.9 m/z ion from this thread: mstdn.science/@sarahehancock/1 is free cyanide reacting with the gold seals of the HPLC pump. I will have to try a few different brands of solvent to see if I can ameliorate this issue. More info also available here: community.agilent.com/technica.

#TeamMassSpec #metabolomics #lipidomics #massspectrometry #massspec

Last updated 1 year ago

Sarah Hancock · @sarahehancock
147 followers · 2 posts · Server mstdn.science

Hey , I'm having issues with a contaminant ion @ 248.9 m/z in -ve ion mode when running 90% ACN 10 mM AmOAc at pH 9. Found a thread on research gate suggesting it comes from the ACN but have tried two brands now with no joy. Has anyone else had this problem?

Research gate link: researchgate.net/post/What-is-

ACN brands tried: Fisher Sci Optima, Supelco LiChroSolv. LiChroSolv has a lot less of it than Fisher Sci.

#TeamMassSpec #metabolomics #lipidomics #massspectrometry #massspec

Last updated 1 year ago

PastelBio · @pastelbio
164 followers · 1618 posts · Server mstdn.science

RT @: Always good to see new tools available.Dear and , I am happy to share HowDirty, an R package that facilitates evaluating and reporting molecular contaminants in LC-MS experiments.
authorea.com/users/643346/arti

#massspec #proteomicscommunity #TeamMassSpec

Last updated 1 year ago

PastelBio · @pastelbio
158 followers · 1504 posts · Server mstdn.science

RT @: Anyone have advice on how they normalize TMT channel data when the samples are from a pulldown? Normalization methods assume that peptide abundances should be broadly equal across samples...

#TeamMassSpec #massspec #proteomics

Last updated 1 year ago

wfondrie · @wfondrie
200 followers · 66 posts · Server genomic.social

I gave a talk on Depthcharge, our toolkit for data at the Cascadia Symposium this week. Here’s my slides in case you missed it, or want to see them again!

👉 zenodo.org/record/8161011

#deeplearning #massspec #proteomics

Last updated 1 year ago

jobRxiv · @jobRxiv
936 followers · 2880 posts · Server mas.to
jobRxiv · @jobRxiv
934 followers · 2839 posts · Server mas.to
PastelBio · @pastelbio
153 followers · 1344 posts · Server mstdn.science

RT @: JULY 15 << The Abstract Deadline is fast approaching for the 2ND INTERNATIONAL TOP-DOWN PROTEOMICS SYMPOSIUM. Submit your work now at> tdp2023.topdownproteomics.org/

#proteomics #ASMS #genomics #massspec #proteoform

Last updated 1 year ago

PastelBio · @pastelbio
147 followers · 1295 posts · Server mstdn.science

RT @: Hey - question re: low sample stage-tip fractionation. Anyone have any experience with it? We have some POROS 20 R2 packing. How much (~mg?) do you load into a 200µL tip?

#TeamMassSpec #massspec #proteomics

Last updated 1 year ago

PastelBio · @pastelbio
136 followers · 1169 posts · Server mstdn.science

RT @: 20,001 DOWNLOADS - The Human Proteoform Project seems to be of interest to quite a few people...
science.org/doi/10.1126/sciadv

#proteomics #proteoforms #genomics #massspec

Last updated 1 year ago

Dr Micha Campbell · @michcampbell
455 followers · 925 posts · Server fediscience.org

Can someone who knows something about mass spectrometry explain why the Agilent 8900 is called a triple quadrupole, when I'm fairly sure there are only two quads??

#massspec #massspectrometry #labwork

Last updated 1 year ago

PastelBio · @pastelbio
132 followers · 1125 posts · Server mstdn.science

RT @: JOB OPPORTUNITY!

Looking for a post-doc developing multiplexed targeted assays to support clinical translation? Look no further!

#massspec #TeamMassSpec #proteomics

Last updated 1 year ago

PastelBio · @pastelbio
131 followers · 1041 posts · Server mstdn.science

MT @: A great opportunity for omics, , & researchers to publish their advances on pushing tech towards single-cell analyses, sensitivity & resolution JPR is not only about proteins -- metabolites, lipids, glycans, drugs, exposome, all welcome!Theo @thalexandrov and I will serve as editors for a Special Issue of @JProteomeRes Single-Cell Omics

Submissions before January 31, 2024 can be included in the special issue.

Information: axial.acs.org/biology-and-biol

#singlecell #massspec #imaging

Last updated 1 year ago

Johannes Rainer · @jorainer
246 followers · 242 posts · Server fosstodon.org

Lecture on proteomics by @lgatto at 2023 including an overview on the :rstats: package ecosystem from

#csama #rformassspectrometry #massspec

Last updated 1 year ago