Polly Fordyce at Stanford
http://www.fordycelab.com/

Question: How do #transcription factors activate transcription?
Outside the DNA binding domain of a TF, there are numerous disordered domains, including activation domains that interact with co-activators--
very little known about that

activation domains are disordered, poorly conserved, and binding to co-activators is very low affinity

developed a #microfluidic platform to measure, tech called STAMMP

#CSSingleCells23

1/2

Biochip Device Tests Malaria Drug Efficacy, Safety

A lab-on-a-chip device with human organ cells is shown in lab tests to distinguish between malaria strains and detect drug toxicity when assessing blood for seven days.

https://sciencebusiness.technewslit.com/?p=44959

#News #Science #Business #Malaria #Parasite #Biochip #MedicalDevice #Preclinical #Engineering #Microfluidic #Blood #Toxicity #Liver #Spleen

We'll be covering #cyborg #algae for making #hydrogen, the effect of #fish poop on #coral, an enzyme used by #bacteria eating bacteria, #fungi compete with bacteria in #microfluidic mazes, and more!

@academicchatter

https://www.youtube.com/watch?v=Njv4vi4-9Po

Do you know those annoying layer lines on #3Dprinted parts? In resin #3Dprinters, they are as small as 25 microns… And we can make #microfluidics molds with these printers. Does this mean we can reach 25 microns features in Z?

YES! We can make a mold for a “staircase” microfluidic device. Now we have steps of 25um on the height of the channel. This #microfluidic does not only filter #microparticles, but also size-sort them. Particles of different sizes will be blocked on different steps.

This week, Diya Fathima started in our research group. Diya has a B.Sc. degree in #Biotechnology and is working on biosensors. She will work in our project #KEAnI. We are developing systems for rapid #antibiogram generation in #nosocomal #infections. The main topics are development of the #microfluidic chip, establishment of staining protocols for the detection of bacteria in the microfluidic chip and chip-based purification of the blood sample (i.e., separation of blood cells).

New #microfluidic device for detecting #SARSCoV2 --can be used for #POC testing, rapid (10") diagnostic https://medicalxpress.com/news/2023-02-microfluidic-device-sars-cov-.html via
@medical_xpress

In vitro systems to study endothelial/pericyte cell interactions

Hydrogel
Collgen/Matrigel/PEG

#Microfluidic #VesselOnAChip

Engineering microvessels

Blood vessel #Organoid
BBB organoid

Dr. Emily Warren & Sharon Gerecht Vascular Biology 2023
https://vb.bioscientifica.com/view/journals/vb/aop/vb-22-0021/vb-22-0021.xml

In a #microfluidic flow cell #pseudomonas were grown on glass and exposed to an #antibiotic gradient (~0.1-9.9x MIC) via 1-channel of a dual inlet. Cells moved towards the abx in a pilG-dependent manner and died. #pyocin expression was observed and this is thought to be a competitive behaviour.

https://doi.org/10.1038/s41467-022-35311-4

@oneabstractaday

In a #microfluidic flow cell #pseudomonas were grown on glass and exposed to an #antibiotic gradient (~0.1-9.9x MIC) via 1-channel of a dual inlet. Cells moved towards the abx in a pilG-dependent manner and died. #pyocin expression was observed and this is thought to be a competitive behaviour.

https://doi.org/10.1038/s41467-022-35311-4

@oneabstractaday

Tumor spheroid-microvasculature-on-a-chip

#Microfluidic
0.6×0.3×25 mm
5 & 15 dyn/cm2
MCF-7 spheroid
HUVEC monolayer

Tumor spheroid alters #EndothelialCell polarization to shear🤠

Sheared EC⏬spheroid growth🤠🤠

Dr. Hongxu Lu & Lining Arnold Ju labs bioRxiv 2022
https://www.biorxiv.org/content/10.1101/2022.11.19.517181v1.full

5-channel (separated by microposts) #Microfluidic for #EndothelialCell-#Pericyte fibrin-
collagen co-culture

Human Lung fibroblast as feeder

Enable study of Synergy b/w #ShearStress & Pericyte on #SproutingAngiogenesis😁

Dr. Ryuji Yokokawa lab, Lab on a chip 2022

Microfluidic Surprised Pikachu, made by a student in my Medical Diagnostics class: #microfluidic #pikachu

#introduction
Bonjour tout le monde,
Lab Manager/technicien sup de recherche, à l'#InstitutPasteur, dans mon équipe, #IndividualitéMicrobienneEtInfection, je suis celui qu'on appelle quand l'espoir doit renaitre : une pipette ou un microscope récalcitrant, une manip foireuse, une épidémie à l'autre bout du monde ou du campus, c'est dans mes gants.
#microfluidic #molecularbiology #virology #mycobacteriumtuberculosis
Également intéressé par les sciences de l'écologie, l'histoire...

Yesterday Danny Ferreira Dias presented his #BSc work on a #microfluidic pumping set-up.

He worked with MF pumps and valves from #Labsmith. Unfortunately, the company’s documentation is rather poor and for the programming a #ReverseEngineering had to be done, i.e. to analyze the commands on the oscilloscope and to translate them into a bit sequence ( 🤔).

Nevertheless, in the end it was possible to control the set-up by a GUI running on a #RasPi. Congratulations for the nice work, Danny!