Our #MidweekMovie is a 360° view through a 3D reconstruction of a cleared E3 chick embryos transfected with TCF7L2 shRNA and labelled with CS2 and Robo3. Antonio Herrera, Sebastian Pons, et al. find that TCF7L2 knockdown decrease the ratio of axons that cross the contralateral tract.
📖 @Dev_journal: https://journals.biologists.com/dev/article/150/16/dev201651/326215/Neurogenesis-redirects-catenin-from-adherens

#DevBio

This movie shows cells leaving behind a glycocalyx patch after detachment.

Part of a recent #preprint from the lab of Shamik Sen #IITBombay showing how “bulky glycocalyx drives cancer invasiveness by modulating substrate-specific adhesion”.

#MidweekMovie

#preprint: https://www.biorxiv.org/content/10.1101/2023.08.03.551677v1

For our #MidweekMovie, we are recommending that you catch up with the #DevPres talks on @the_node. The speakers, Hannah Gruner, Seth Donoughe & @allan_littlecar all use microscopy to address research questions in #evodevo.

https://thenode.biologists.com/development-presents-august-evo-devo-webinar/

#DevBio

Our #MidweekMovie follows the development of a chimaeric mouse embryo from the 8-cell stage to the blastocyst stage. Anna Soszyńska, Aneta Suwińska and colleagues use these ESC-chimaeric embryos to explore the regulatory mechanisms that preserve the balance between cell lineages.
📖 @Dev_journal: https://journals.biologists.com/dev/article/150/14/dev201756/324828/Multi-level-Fgf4-and-apoptosis-dependent

#microscopy #devbio

Our #MidweekMovie shows #Drosophila larval and cardiac valves upon #metamorphosis from Christian Meyer, Achim Paululat and colleagues. They dissect the differentiation and function of the cardiac valves in the fly.
📖 @J_Exp_Bio: https://journals.biologists.com/jeb/article/226/13/jeb245839/322685/Differentiation-and-function-of-cardiac-valves-in

#microscopy #development

Our #MidweekMovie shows blood flow in control, alas2-/- and alad-/- zebrafish embryos. Peng Lv and Feng Liu find that while the number of circulating RBCs is normal in these mutants, survival of HSPCs is impaired.

📖 @Dev_journal: https://journals.biologists.com/dev/article/150/20/dev201690/316750/Heme-deficient-primitive-red-blood-cells-induce

Our aMAZ(E)ing #MidweekMovie comes from Connie Shen, Judith Mandl, Nienke Vrisekoop and colleagues. They discover that neutrophils with segmented nuclei have a speed advantage when migrating through confinement spaces

📖 @J_Cell_Sci: https://journals.biologists.com/jcs/article/136/11/jcs260768/316606/Nuclear-segmentation-facilitates-neutrophil

Our #MidweekMovie shows actin dynamics in a #zebrafish embryo from Haruka Oda, Hiroshi Kimura and colleagues. They find that filamentous actin accumulates in the nucleus of early embryos and forms patches associating with condensing chromosomes during prophase.

📖 @BiologyOpen https://journals.biologists.com/bio/article/12/5/bio059783/310414/Actin-filaments-accumulated-in-the-nucleus-remain

Our #MidWeekMovie shows adult-type fat body precursor cells as they begin to migrate dorsally from the #Drosophila abdomen, 30h APF. Taiichi Tsuyama, Tadashi Uemura and colleagues track the behaviour of AFBp cells during metamorphosis.

📖 @Dev_journal: https://journals.biologists.com/dev/article/150/10/dev200815/310416/Dynamic-de-novo-adipose-tissue-development-during

In a departure from our usual #MidweekMovie, this week we are looking at #bumblebee flight! Stacey Combes, Nick Gravish and Susan Gagliardi find that bumblebees prefer flying upwind and body angle & speed is more variable for downwind flight.

📖 @J_Exp_Biol: https://journals.biologists.com/jeb/article/226/Suppl_1/jeb245374/307071/Going-against-the-flow-bumblebees-prefer-to-fly

The beauty of #zebrafish skin! Credit: @errricpeterman
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RT @focalplane_jcs
Our #MidweekMovie show mpeg1+ Langerhans cells engulfing debris during axon degeneration. @errricpeterman @jraslab @UWBiology, et al. present a new model for studying axon degeneration with high spatiotemporal resolution.
📖@DMM_Journal: https://journals.biologists.com/dmm/article/16/4/dmm049911/306224/Zebrafish-cutaneous-injury-models-reveal-that
https://twitter.com/focalplane_jcs/status/1643615918172381186

Our #MidweekMovie shows displacement of germ cell vertices after a laser cut in ctrl and Cap-1 RNAi worms.. Shinjini Ray, Priti Agarwal, Ronen Zaidel-Bar, et al. discover a role for CAP-1 in regulating actomyosin contractility in reproductive tissue.
@Dev_journal: https://journals.biologists.com/dev/article/150/6/dev201099/297299/Actin-capping-protein-regulates-actomyosin

Our #MidweekMovie shows TIRFM single molecule imaging of 27 transmembrane proteins in Dictyostelium from Kazutoshi Takebayashi, Yoichiro Kamimura and Masahiro Ueda, where they found that membrane viscosity is the main determinant of lateral diffusion.
https://journals.biologists.com/jcs/article/136/4/jcs260280/287060/Field-model-for-multistate-lateral-diffusion-of

You can find out more about Kazutoshi in our ‘First person’ interview: https://journals.biologists.com/jcs/article/136/4/jcs261050/287079/First-person-Kazutoshi-Takebayashi

Our #MidweekMovie is from Franziska Lehne and Sven Bogdan who find that loss of Swip-1 in the Drosophila salivary gland impairs cargo expulsion of glue granules during regulated secretion.

@J_Cell_Sci: https://journals.biologists.com/jcs/article/136/6/jcs260366/294501/Swip-1-promotes-exocytosis-of-glue-granules-in-the

You can find out more about the research in the ‘First person’ interview with Franziska Lehne:
https://journals.biologists.com/jcs/article/136/6/jcs261106/294500/First-person-Franziska-Lehne

#cellbiology #membranetrafficking

Take that! Our #Midweekmovie comes from Jacob S Harrison and S. N. Patek shows the ultrafast strike of a juvenile snapping shrimp; the cavitation bubble, produced from the click of the spring-loaded claw, implodes with a pop and a flash of light to incapacitate their opponent.

@J_Exp_Biol: https://journals.biologists.com/jeb/article/226/4/jeb244645/287686/Developing-elastic-mechanisms-ultrafast-motion-and

#microscopy #snappingshrimp

Our #MidweekMovie examines the behaviour of neutrophils and macrophages following tail transsection in #zebrafish embryos. The #OpenAccess study from Maaike Allers, Petra Bakker, Jeroen den Hertog and colleagues finds that Shp1, mutations of which are associated with emphysema in humans, is important for neutrophil and macrophage function.

@DMM_Journal: https://journals.biologists.com/dmm/article/16/2/dmm049715/286800/Loss-of-Shp1-impairs-myeloid-cell-function-and

In our #MidweekMovie we look at germband extension in Drosophila embryos.

In their @Dev_journal paper, Lenin Chandran, @luschnig , H.-Arno Müller and colleagues find that Src42A is required for E-cadherin dynamics during axis elongation.

https://doi.org/10.1242/dev.201119
#WhiteLightWednesday #devbio #microscopy

#midweekMovie to be behold by the intracellular world

Would #organelle peops agree with me that this is a multivesicular body on the move? #MVB #cell

(Maybe @GoetzJacky @French_Soc_EVs @guillaume_niel @JKlumperman @NalanLiv @Endo_Connect would know)
@focalplane_jcs @cellcommlab