8/n last - Study funding was by @boehringerus@twitter.com #DiscoveryAward to @Farida_Ahangari@twitter.com & by @nih_nhlbi@twitter.com to me & others, also supported by institutional cores, colleagues, patient advocates, sample & tissue donors
Thank you all!!!
#CureIPF #miR33PFPaper
https://insight.jci.org/articles/view/158100

7/n So grateful to the team, led by Dr. Ahangari, #KaminskiLab members, so many selfless collaborators including Drs Fernández-Hernando, Price, Dela Cruz, Prasse, Ivan Rosas, & Bahal (https://bahal.lab.uconn.edu/ that designed PNA-33) #CureIPF #miR33PFPaper
https://insight.jci.org/articles/view/158100

6/n Pharmacologic inhibition of miR-33 using PNA-33 in precision-cut lung slices obtained from bleomycin-injured mice or humans with IPF, also indicated antifibrotic effects, including reversal of profibrotic macrophage signature
#CureIPF #miR33PFPaper
https://insight.jci.org/articles/view/158100

5/n Impressively, loss of Mir-33 in myeloid cells, has an effect on alveolar type II cells, their mitochondrial homeostasis improved and apoptosis ⬇️
#CureIPF #miR33PFPaper
https://insight.jci.org/articles/view/158100

4/n Loss of miR-33 in macrophages improves mitochondrial homeostasis (function and structure) at baseline and after bleomycin injury, ⬆️ autophagy and mitophagy #CureIPF #miR33PFPaper
https://insight.jci.org/articles/view/158100

3/n Knocking down miR-33 genetically in myeloid cells, or pharmacologically using a Peptide Nucleic Acid inhibitor designed by Dr. Bahal (PNA-33) protects mice against bleomycin-induced Pulmonary Fibrosis
#CureIPF #miR33PFPaper
https://insight.jci.org/articles/view/158100

2/n We first show that miR-33 is increased in BAL and lung cells obtained from IPF patients and that miR-33 target genes are decreased in IPF
#CureIPF #miR33PFPaper
https://insight.jci.org/articles/view/158100

1/n Our first #CurePF paper for 2023 is OUT! A novel role in Pulmonary Fibrosis for miR-33, a key regulator of macrophage immuno-metabolic response & mitochondrial function, providing evidence that inhibition may be antifibrotic
#miR33PFPaper
https://doi.org/10.1172/jci.insight.158100