Just read "Random genetic drift sets an upper limit on mRNA splicing accuracy in metazoans" by Benitiere et al @duret_lbbe, very nice and careful argument that most alternative splicing in e.g. vertebrates is non functional and accumulates neutrally. https://doi.org/10.1101/2022.12.09.519597 #MolecularEvolution #AlternativeSplicing #Neutralism