Really cool preprint and prelight. But my question is how can you be sure that the expansion is homogenous across a protein? Proteins are structurally heterogenous so I would assume they expand at diff. rates. Same for cellular structures.
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RT @preLights
The shape of single proteins seen with light microscopy. #ONE_Microscopy combines the power of expansion microscopy with SRRF analysis. 🔬

https://prelights.biologists.com/highlights/…
https://twitter.com/preLights/status/1596079351296147457

Check out the latest #post @preLights? 📜👀

https://prelights.biologists.com/highlights/expansion-microscopy-at-one-nanometer-resolution/

#preLighter Nadja Hümpfer covers a #preprint which describes the combination of #SRRF analysis with 10-fold expansion microscopy #ExM. 🔬 Their approach allows a final resolution of 1 nm; a #resolution at which the shape of individual proteins becomes apparent when labelled with a pan-protein staining.

This preLight also includes comments from #authors Ali H. Shaib & Silvio O. Rizzoli 📝

#ONE_Microscopy #immunoglobulin