Scientists present a computational strategy that mimics gene duplication to predict new #ProteinProteinInteractions—enabling exploration of uncharted regions of the protein sequence space and design of new interacting protein pairs. https://scim.ag/2b8 @scisignal #press

A new proteomic workflow establishing interdependencies between protein #phosphorylation & complex formation is applied to the signal integrator & Hippo pathway effector YAP1 ➡️ http://bit.ly/3mJ4W0t
Matthias Gstaiger @eth_en
#proteomics #interactome #ProteinProteinInteractions #CellSignaling

News &Views by
P Kastritis on the recent study from the Rappsilber lab identifying bacterial PPIs in their cellular context by combining whole-cell crosslinking, co-fractionation #massspectrometry & AI-based PPI structure prediction ➡️http://bit.ly/3FfcIVY
#proteomics #ProteinProteinInteractions #SystemsBiology #Alphafold

An integrative approach using crosslinking #massspectrometry (MS), co-fractionation MS & #Alphafold-Multimer discovers new protein complexes & their topologies in B.subtillis ➡️ http://bit.ly/3kpjocU
from Juri Rappsilber (TU Berlin), Jörg Stülke (Uni Goettingen)
#proteomics #SystemsBiology #ProteinProteinInteractions

ABOLISH: an optimised biotin-ligation method for discovering stable & transient #ProteinProteinInteractions in yeast ➡️ bit.ly/3ktzuBT
from Maya Schuldiner's lab, Weizmann Institute
#SystemsBiology #Scerevisiae