.@SarinayElif &co reveal a novel mechanism of growth coordination in #Celegans; inhibition of #ribosome biogenesis in epidermis can trigger organism-wide #GrowthQuiescence, potentially via secreted proteins & the dense-core vesicle pathway #PLOSBiology https://plos.io/3R1jYvq

@JoachimFrank

Wow: “In an operating range from 10 to 1000 ms, the device allows us to follow in vitro reactions of biological molecules at resolution levels in the range of 3 Å. By employing this method, we show for the first time the mechanism of progressive HlfX-mediated splitting of the 70S E. coli ribosome in the presence of the GTP, via capture of three high-resolution reaction intermediates within 140 ms.” —Bhattacharjee et al. 2023 https://www.biorxiv.org/content/10.1101/2023.01.25.525430v2.full

#StructuralBiology #CryoEM #ribosome

Protein Synthesis – A Molecular Happening
by Roberto
More than fifty years ago, towards the end of Spring term in May 1971, university campuses across the U.S. were rife with protests over the Vietnam war. Students skipped classes and left labs to participate in marches and sit-ins. In addition, the memory of Woodstock (August 1969) was still fresh in everyone's mind.

Read more → https://schaechter.asmblog.org/schaechter/2023/08/protein-synthesis-a-molecular-happening.html

#microbiology #translation #ribosome

An #ERK1/2-driven RNA-binding switch in #nucleolin drives #ribosome #biogenesis and #pancreatic #tumorigenesis downstream of RAS #oncogene
Faraz K Mardakheh and collaborators
https://www.embopress.org/doi/10.15252/embj.2022110902

Now when I see this image of a #ribosome, its yellow-blue palette and top-bottom structure really reminds me of #Python.

(Image source: https://www.xray.cz/setkani/abst2023/556.htm)

Referenced link: https://phys.org/news/2023-04-humans-bacteria-differences-ribosome-decoding.html
Discuss on https://discu.eu/q/https://phys.org/news/2023-04-humans-bacteria-differences-ribosome-decoding.html

Originally posted by Phys.org / @physorg_com: http://nitter.platypush.tech/physorg_com/status/1643661544775995392#m

Humans vs. bacteria: Differences in #ribosome decoding revealed @nature https://www.nature.com/articles/s41586-023-05908-w https://phys.org/news/2023-04-humans-bacteria-differences-ribosome-decoding.html

Relationship status: *It's complicated*🤷

Lee et al. work on untangling how the targeting specificity of signal-anchored #proteins is determined for specific targeting to #mitochondria and #chloroplasts.
https://doi.org/10.1111/jipb.13475
@wileyplantsci
#PlantScience #JIPB #ribosome #mRNA

Translation of viral #RNAs is often driven by non-canonical events, which are encoded in the viral genome to hijack the cellular translation machinery. One such event is programmed ribosomal #frameshifting. It prompts the translating #ribosome to slip one nucleotide back or forth into a different reading frame, enabling viral replication. Researchers from the Helmholtz Institute Würzburg unveiled the structural transitions in the #SARSCoV2 frameshifting process. 🧬 👩‍🔬 🦠
➡️ https://www.helmholtz-hiri.de/en/newsroom/news/detail/news/decoding-sars-cov-2/

All the fun keywords in our new preprint!: #bioinformatics #microbiology #toxins #antitoxins #phage #AlphaFold. I'm even going to throw in #ribosome for good measure.
https://twitter.com/gem__atkinson/status/1638766346786820096

RT @RoliRoberts
.@Mukherjee_Lab describes sidI, an intriguing effector secreted by #Legionella - this proteinaceous tRNA mimic mannosylates ribosomes and eEF1a, triggering #ribosome collision, nuclear accumulation of ATF3, and host cell death #EESSymbiosis

Interesting!
Anorher paper looking at #ribosome heterogeneity, in mice.

"Dynamic interplay between RPL3- and RPL3L-containing ribosomes modulates mitochondrial activity in the mammalian heart"

#translation #mitochondria

https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkad121/7068369

Online now - our 2023 issue 5:
Activation of #plant #NLR #immune receptors
#Stereoisomer specificity in #flagellin #sialylation
Dormant #ribosome activation by #TORC1
#Cytomegaloviral #DCAF for #cullin-mediated #STAT2 degradation
Cover: Korolchuk lab
https://www.embopress.org/toc/14602075/2023/42/5

New paper published in Nature Communications describing how Eterna players redesigned the E. coli #ribosome to produce 42% more protein in iSAT experiments.

https://www.nature.com/articles/s41467-023-35827-3

I sure would like to have this arcade game in my basement! @amw_stanford

#RNA #science #synbio #biochemistry

"We identified and validated APOBEC3A and APOBEC4 as novel #ribosome biogenesis factors through our laboratory's established screening platform for the discovery of regulators of nucleolar function in MCF10A cells."

#Apobec #preprint

https://www.biorxiv.org/content/10.1101/2023.02.20.529126v1

"We identified and validated APOBEC3A and APOBEC4 as novel #ribosome biogenesis factors through our laboratory's established screening platform for the discovery of regulators of nucleolar function in MCF10A cells."

#Apobec

https://www.biorxiv.org/content/10.1101/2023.02.20.529126v1

Beautiful work.
---
RT @BeckmannLab
Check our most recent work in collaboration with the amazing @inada_lab. The study unveils the molecular basis of eIF5A-dependent CAT tailing in eukaryotic ribosome-associated quality control. #ribosome #cryoEM #MolecularCell Here's a thread with the most exciting findings🧵...
https://twitter.com/BeckmannLab/status/1626536169642110976

#RIP Paul Berg (1926-2023)

(Paul Berg) Protein Synthesis: An Epic on the Cellular Level

the best video ever

In 1971, several hundred Stanford University students convened to undulate and impersonate molecules undergoing #protein #synthesis by a #ribosome. https://youtu.be/WTRmvnlNVw4

A new way to make human cell-free translation systems. These are highly active and can form robust polysomes. They open new avenues for studying human translation mechanisms. #Ribosome
https://www.biorxiv.org/content/10.1101/2023.02.09.527910v1

Looking for a weekend read? How about Ulrike Kutay et al’s contribution to our 2023 RNA #Review Series:
#Ribosome #biogenesis factors—from names to functions
https://www.embopress.org/doi/10.15252/embj.2022112699

This can be useful:
"Learning to Generate 5’ UTR Sequences for Optimized Ribosome Load and Gene Expression"

@biorxivpreprint
#mRNA #translation #5UTR #ribosome

https://www.biorxiv.org/content/10.1101/2023.01.30.526198v2