Iva Lučić shows our students how to label CaMKII with fluorescent dye for #singleMolecule TIRF #microscopy #biophysics

This is really cool- great to see #singlemolecule *molecular* scale behaviour inside #condensates: https://www.nature.com/articles/s41586-023-06329-5

Hello! A new intro for a new instance.

I lead a research group trying to figure out axonal transport and neurodegeneration. A big emphasis on the microtubules and their motors, but really the transport of anything that doesn't have a membrane around it. For this we use a lot of single molecule methods and live cell imaging. I also post about research culture.

#introduction #kinesin #dynein #microtubules #neurodegeneration #AxonalTransport #neurobiology #SingleMolecule #CellBiology

🧐 #MeetTheSpeaker!

🔬 What are the next advancements in the field of #singlemolecule sequencing technologies? We talked about it with Francesco Nicassio (Istituto Italiano di Tecnologia)

“Only together we can really find new solutions”

#science #sciencemastodon #lifesciences #humantechnopole https://www.youtube.com/watch?v=JihuHbYmdYk

Our NIH #R35 #MIRA has been awarded! I'm glad to be continuing collaborations within #WashU #McKelveyEngineering, and pushing our development of #6D #singlemolecule #nanoscopy.

https://engineering.wustl.edu/news/2023/Looking-deeper-with-adaptive-six-dimensional-nanoscopy.html

PhD Position - Acoustofluidic Single-molecule Nanosystems

#PhDvacancy on #nanodevices and #singlemolecule biophysics. Join our interdisciplinary team at #TUDelft in 2023!

https://jobrxiv.org/job/tu-delft-27778-phd-position-acoustofluidic-single-molecule-nanosystems/?feed_id=44208

#ScienceJobs #job
Delft #Netherlands #PhDStudent
https://jobrxiv.org/job/tu-delft-27778-phd-position-acoustofluidic-single-molecule-nanosystems/?feed_id=44208

🧐 #MeetTheSpeaker!

🔬 How can single molecule sequencing technologies change research on the human genome? Listen to Davide Bolognini, Senior Bioinformatician in our Genomics Research Centre.

“International collaborations, of course, are an essential part for developing new methods”

#lifesciences #humantechnopole #genomics #singlemolecule #sequencing #humangenome #science #sciencemastodon

https://youtu.be/Ka85RhFkPOU

PhD Position - Acoustofluidic Single-molecule Nanosystems

#PhDvacancy on #nanodevices and #singlemolecule biophysics. Join our interdisciplinary team at #TUDelft in 2023!

https://jobrxiv.org/job/tu-delft-27778-phd-position-acoustofluidic-single-molecule-nanosystems/?feed_id=42570

#ScienceJobs #job
Delft #Netherlands #PhDStudent
https://jobrxiv.org/job/tu-delft-27778-phd-position-acoustofluidic-single-molecule-nanosystems/?feed_id=42570

PhD Position - Acoustofluidic Single-molecule Nanosystems

#PhDvacancy on #nanodevices and #singlemolecule biophysics. Join our interdisciplinary team at #TUDelft in 2023!

https://jobrxiv.org/job/tu-delft-27778-phd-position-acoustofluidic-single-molecule-nanosystems/?feed_id=41694

#ScienceJobs #job
Delft #Netherlands #PhDStudent
https://jobrxiv.org/job/tu-delft-27778-phd-position-acoustofluidic-single-molecule-nanosystems/?feed_id=41694

"Boosting the toolbox for live imaging of translation"

The authors developed a new set of #suntag tools (differnt green fluorescent proteins, different promoter for scFv) - optimizing them and identifying possible artefacts. these are primarily for #Drosophila but I guess could be adapted to other organisms.

They also developed a new system called ALFA_array which they test in fly and mammalian cells.

#mRNA #translation #microscopy #LiveCellImaging #SingleMolecule

https://www.biorxiv.org/content/10.1101/2023.02.25.529998v1

#bps2023 Come see @Tingting_Wu_ tell a beautiful story on “Mapping Inhomogeneous Network Structures within Biomolecular Condensate using #SingleMolecule Imaging and Tracking of Fluorogenic Probes,” tomorrow at 9:15 AM in Room 6C, a collaboration with #PappulabWashU

We'll also be presenting “A Multi-View Reflector Microscope Visualizes #SixDimensional #SingleMolecule Translational And Rotational Dynamics With Isotropic Nanoscale Resolution!” Poster 1351-Pos / B480 #bps2023 https://doi.org/10.1016/j.bpj.2022.11.1587

#bps2023 Come hear Brian talk about “#SingleMolecule Orientation-Localization Microscopy Resolves How Amyloidophilic Dye Orientations are Correlated with Amyloid Fiber Growth and Disruption,” Poster 1339-Pos / B468 tomorrow at 1:45 PM! https://doi.org/10.1016/j.bpj.2022.11.1575

#bps2023 Come visit Weiyan tomorrow as she presents “#SingleMolecule Orientation-Localization Microscopy Resolves the Nanoscale Organization of Self-Assembled Peptides,” Poster 627-Pos / B448 at 1:45 PM in Exhibit Hall BC https://doi.org/10.1016/j.bpj.2022.11.863

This just published from Jeff Chao's lab
@MolecularCell

"Single-molecule imaging reveals translation-dependent destabilization of mRNAs"

#mRNA #mRNADegradation #miRNA #translation #imaging #SingleMolecule

https://www.cell.com/molecular-cell/fulltext/S1097-2765(23)00033-3

When the bioRxiv came out (along with 2 others on similar topic), I wrote about it in my blog:

https://greenfluorescentblog.wordpress.com/2021/07/25/imaging-of-mirna-mediated-translational-repression-and-mrna-decay-at-single-molecule-resolution/

There are several additions in the print version, e.g. adding miR-21. but its just 1st impression. Have to read it.

Happy to share new collaborative work on #singlemolecule imaging of biomolecular #condensates. They're less uniform than they seem! With #PappulabWashU @TingtingWu #MattKingLab #MinaFaragSci #WashUengineers

"Single fluorogen imaging reveals spatial inhomogeneities within biomolecular condensates"
https://doi.org/10.1101/2023.01.26.525727

🔬 Calling all scientists! Join us at the Symposium on Single Molecule Sequencing Techniques, co-organized by HT and IIT. Discover the latest advancements in a revolutionary technique for DNA/RNA sequencing with ultra-long strands that has just been named Method of the Year.

Register now and join the discussion 👉 https://humantechnopole.it/en/trainings/single-molecule-sequencing-technologies-applications/

#singlemolecule #sciencemastodon #science #genomics #lifesciences #humantechnopole #nanopore @mattloose @nanopore

RT @RiveraChemLab
We have a postdoc position to work on the further development of our #singlemolecule #proteomics method blinkognition. For details and to submit your application submission, please visit: https://tinyurl.com/3wr7k3hr
Please RT. https://youtu.be/gEZOPI4Nm_k

We're looking for a postdoc in Low Energy Electron Holography! The deadline to apply is Jan 15! Learn more at: https://fkf.mpg.de/7879580/PostDoc-LEEH-12_22_1.pdf

#ElectronMicroscopy #TEM #cryoem #teammassspec #singlemolecule #structuralbiology #postdocopening #postdocjobs #postdoc

RT @RiveraChemLab
Blinkognition is a new method to identify #peptides with #singlemolecule sensitivity. Why is this important? Because it is a first step towards ultrasensitive #proteomics, well beyond the current capabilities of mass spectrometry. 1/4